MSP1594_2x_NLS
(Plasmid
#110625)
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PurposeMammalian expression vector for Streptococcus thermophilus CRISPR1 (St1Cas9 LMD-9)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 110625 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneMSP1594 (Plasmid #65775)
- Total vector size (bp) 8282
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Vector typeMammalian Expression, CRISPR, Synthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert 1
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Gene/Insert nameSt1Cas9 LMD-9
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SpeciesH. sapiens (human), Synthetic
- Promoter CAG
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Tags
/ Fusion Proteins
- SV40 NLS (N terminal on insert)
- SV40 NLS (C terminal on insert)
Cloning Information for Gene/Insert 1
- Cloning method Gibson Cloning
- 5′ sequencing primer oBK280 (5'-CCTACAGCTCCTGGGCAACG-3') (Common Sequencing Primers)
Gene/Insert 2
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Gene/Insert nameNone
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SpeciesSynthetic
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
This plasmid is identical to MSP1594 (Plasmid #65775) except for the addition of an SV40 NLS to the N-terminus of mammalian codon-optimized Streptococcus thermophilus CRISPR1-Cas9 LMD-9. Please visit https://www.biorxiv.org/content/early/2018/05/14/321208 for BioRxiv preprint
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
MSP1594_2x_NLS was a gift from Yannick Doyon (Addgene plasmid # 110625 ; http://n2t.net/addgene:110625 ; RRID:Addgene_110625) -
For your References section:
Versatile and robust genome editing with Streptococcus thermophilus CRISPR1-Cas9. Agudelo D, Carter S, Velimirovic M, Duringer A, Rivest JF, Levesque S, Loehr J, Mouchiroud M, Cyr D, Waters PJ, Laplante M, Moineau S, Goulet A, Doyon Y. Genome Res. 2020 Jan;30(1):107-117. doi: 10.1101/gr.255414.119. Epub 2020 Jan 3. 10.1101/gr.255414.119 PubMed 31900288