-
Plasmid#157872Purposeexpress MBP tagged SARS-CoV-2 Nucleocapsid protein without the C-terminal IDRDepositorInsertN (N SARS-CoV-2)
UseTagsMBPExpressionBacterialMutationdeltaCIDRPromoterAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pVenus-Sec16A
Plasmid#36157PurposeExpresses Sec16A as a Venus FP fusionDepositorInsertSec16A (SEC16A Human)
UseTagsVenus (EYFP derivative)ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pENTR-Arl13b1
Plasmid#40870DepositorInsertArl13b (Arl13b Mouse)
UseGateway entryTagsExpressionMutationPromoterAvailable sinceOct. 29, 2012AvailabilityAcademic Institutions and Nonprofits only -
pJB045 (ZapB-mEos2)
Plasmid#72651PurposeInducible expression of ZapB-mEos2 in bacteriaDepositorInsertZapB-mEos2
UseTagsmEos2ExpressionBacterialMutationPromoterT5-lacAvailable sinceFeb. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCMV-FKBP-mCherry-Vav2cat
Plasmid#178865PurposeMammalian expression of Vav2(DH-PH-CRD) fused to FKBP-mCherryDepositorInsertFKBP-mCherry-Vav2(DH-PH-CRD) (Vav2 Mouse)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIRESpuro-Flag-Pol b(K72A)
Plasmid#23258DepositorInsertDNA polymerase beta (K72A) (POLB Human)
UseTagsFlagExpressionMammalianMutationK72APromoterAvailable sinceMarch 31, 2010AvailabilityAcademic Institutions and Nonprofits only -
-
pNS3
Plasmid#131770PurposePADH1_FUSN_Fusion Red_Cry2Olig_TACT1 for Integration into δ-sitesDepositorInsertPADH1_FUSN_Fusion Red_Cry2Olig_TACT1
UseIntegration into δ-sitesTagsExpressionYeastMutationE490G to make Cry2 into Cry2OligPromoterPADH1Available sinceSept. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET:GACG:GFP
Plasmid#60735PurposeA modified pET28 driving expression of GFP, biobrick part Bba_E0040. No LacO site downstream of PT7. T7 promoter has base pairs 'GACG' between -11 and -8.DepositorInsertGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceNov. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET21a-mAIM2-deltaPYD
Plasmid#51535PurposeExpress His6-tagged HIN domain of mAIM2 in bacteriaDepositorInsertAim2 (Aim2 Mouse)
UseTagsHis6 and T7ExpressionBacterialMutationPromoterlac operatorAvailable sinceMarch 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1-T7-hAIM2
Plasmid#51541PurposeExpress T7 and GFP-tagged hAIM2 in mammalian cellsDepositorInsertAIM2 (AIM2 Human)
UseTagsGFP and T7ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAF256
Plasmid#105496PurposepRPF185 derived plasmid encoding an anhydrotetracyclin inducible HupA-SmBiT and LgBiTDepositorInsertHupA-SmBiT/LgBiT
UseAtc-dependent expression in c. difficileTagsExpressionMutationPromoterPtetAvailable sinceSept. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-2-Zfp36-3'UTR
Plasmid#61780PurposeLuciferase reporter containing the 3'UTR of mouse Zfp36DepositorInsertmouse Zfp36 3'UTR
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceMay 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
minitalin (headR13DD)
Plasmid#182994Purposeexpression of minitalin wt in mammalian cellsDepositorInsertminitalin (Tln1 Mouse)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceNov. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
talin1-head
Plasmid#182996Purposeexpression of talin1 head wt in mammalian cellsDepositorInserttalin1 head (Tln1 Mouse)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceNov. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
TAL-L1-Tf1-Ctrl
Plasmid#103036PurposePlasmid for IvT reaction with T3. Encodes LINE-1 specific TALE sequence followed by multi cloning siteDepositorInsertTAL-L1-Tf1
UseT3 in vitro transcription for expression in mamma…TagsFLAG and HAExpressionMutationPromoterAvailable sinceNov. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pNS1
Plasmid#131769PurposePADH1_FUSN_Fusion Red_Cry2WT_TACT1 for Integration into δ-sitesDepositorInsertFUSN_Fusion Red_Cry2WT
UseIntegration into δ-sitesTagsExpressionYeastMutationPromoterPADH1Available sinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pIS1 Cyclin D2 long UTR
Plasmid#21645DepositorInsertCyclin D2 long UTR (CCND2 Human)
UseLuciferaseTagsLuciferaseExpressionMammalianMutationPromoterAvailable sinceJuly 22, 2009AvailabilityAcademic Institutions and Nonprofits only -
pBW414hrpL-cIgfp
Plasmid#61439PurposepSB4A3 harbouring hrpL-rbs34-cI-lva-ter-Plam-rbs30-gfp-terDepositorInsertHrpL-rbs34-CI-LVA-t-plam-30gfp-t
UseTagsLVAExpressionBacterialMutationPromoterHrpLAvailable sinceAug. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
CAG-RARa-VP16
Plasmid#185559PurposeUbiquitous expression of constitutively active Retinoic Acid Receptor alphaDepositorInsertRARa-VP16 (Rara Mouse)
UseTagsVP16ExpressionMammalianMutationPromoterCAGAvailable sinceJuly 18, 2022AvailabilityAcademic Institutions and Nonprofits only