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Plasmid#8548DepositorInsertHOXD4 (Hoxd4 Mouse)
UseTagsHis and T7ExpressionBacterialMutationmakes protein by Western and by in vitro transcri…PromoterAvailable sinceMay 30, 2006AvailabilityAcademic Institutions and Nonprofits only -
NG1301 pCI-TPI-4MS2-SMG5(short)-4H
Plasmid#65806PurposeNMD reporter mRNADepositorInsertTPI (TPI1 Human)
UseTags4 MS2 stem loops, SMG5 3'UTR shortened versi…ExpressionMammalianMutationPromoterCMVAvailable sinceSept. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1a-V5-Mfsd10
Plasmid#120238PurposeExpresses V5-tagged Mfsd10 in lentiviral vectorDepositorInsertMfsd10 (Mfsd10 Mouse)
UseLentiviralTagsV5ExpressionMutationPromoterEF1aAvailable sinceJan. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBABEhygro-Flag-ZO-2-delta-N
Plasmid#27476DepositorInsertZonula Occludens 2 (TJP2 Human)
UseRetroviralTagsFlagExpressionMammalianMutationDeletion of coding nucleotides from 1-351. This r…PromoterAvailable sinceMarch 31, 2011AvailabilityAcademic Institutions and Nonprofits only -
GFP-DroshaS300A
Plasmid#62526PurposeSerine 300 of Drosha is mutated to alanineDepositorInserthuman Drosha with serine 300 changed to alanine (DROSHA Human)
UseTagsGFPExpressionMammalianMutationchanged serine 300 to alaninePromoterAvailable sinceMarch 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
GFP-DroshaS302A
Plasmid#62527PurposeSerine 302 of Drosha is mutated to alanineDepositorInserthuman Drosha with serine 302 changed to alanine (DROSHA Human)
UseTagsGFPExpressionMammalianMutationchanged serine 302 to alaninePromoterAvailable sinceMarch 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
PD-1-miSFIT-1-A, 5-A
Plasmid#124682PurposeTuning expression of PD-1DepositorInsertPD-1 (PDCD1 Human)
UseTagsExpressionMammalianMutationPromoterminimal CMVAvailable sinceMay 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
NFATc2-CRISPR #2 (PX458-EF1a-pSpCas9(BB)-2A-GFP)
Plasmid#75233PurposeCRISPR/Cas9 plasmid against human NFATc2DepositorInsertsgRNA against NFATc2 (NFATC2 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5 FRT TO myc Separase delta 55/S1126A
Plasmid#59825PurposeAllows the integration of myc Separase delta 55/S1126A in the genome and Tet-inducible expression.DepositorInsertSeparase (ESPL1 Human)
UseTagsMycExpressionMammalianMutationdelta 55/S1126APromoterhybrid human cytomegalovirus (CMV)/TetO2 promoterAvailable sinceMay 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBacPak-HA-Non-stop
Plasmid#52289PurposeExpresses Drosophila Non-stopDepositorInsertNon-stop (not Fly)
UseTagsHAExpressionInsectMutationPromoterAvailable sinceApril 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
HOXB9 (murine) HIS-tag pET
Plasmid#8541DepositorInsertHOXB9 (Hoxb9 Mouse)
UseTagsHis and T7ExpressionBacterialMutationMissing the first 13 amino acids of HOXB9; makes …PromoterAvailable sinceMay 30, 2006AvailabilityAcademic Institutions and Nonprofits only -
sgGCAT_2
Plasmid#72885PurposeCRISPR-Cas9 induced gene disruptionDepositorInsertsgGCAT
UseLentiviralTagsExpressionMutationPromoterCMVAvailable sinceFeb. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMGF170
Plasmid#96997Purposeish1-mCherry insertion cassette with NAT resistance geneDepositorInsertish1 (ish1 Fission Yeast)
UseTagsGST and mCherryExpressionBacterialMutationish1 C-terminal domain fused with mCherry + NAT r…PromoterN/AAvailable sinceAug. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBiex PKC - Peptide 14 mCit mCer
Plasmid#83565PurposeExpresses a mCitrine-mCerulean FRET sensor containing the catalytic domain of PKCalpha and a short peptide substrate derived from the pseudosubstrate of PKCalpha.DepositorInsertPKC alpha (PRKCA Human)
UseTags10 nm ER/K Helix, FLAG Purificaiton Tag, Tev Prot…ExpressionBacterial and InsectMutationOnly catalytic domain of PKC (aa 335-672)PromoterT7 PromoterAvailable sinceNov. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
MDH1-miR-221SM-PGK-GFP-Blast
Plasmid#31520DepositorInsertmiR-221 (Mir221 Mouse)
UseRetroviralTagsGFPExpressionMammalianMutationseed mutant controlPromoterAvailable sinceSept. 21, 2011AvailabilityAcademic Institutions and Nonprofits only -
MDH1-miR-196a-1SM-PGK-GFP-Blast
Plasmid#31510DepositorInsertmiR-196a1 (Mir196a-1 Mouse)
UseRetroviralTagsGFPExpressionMammalianMutationseed mutant controlPromoterAvailable sinceSept. 19, 2011AvailabilityAcademic Institutions and Nonprofits only -
Hoxa4-3'UTR
Plasmid#31524DepositorInsertHoxa4-3'UTR (Hoxa4 Mouse)
UseLuciferaseTagsLuciferaseExpressionMammalianMutationPromoterAvailable sinceOct. 4, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCVL.SSA TLR (Ani)
Plasmid#45577PurposeCodes for the SSA-TLR with the target site for I-Ani I in a lentiviral backboneDepositorInserts5' iRFP arm
eGFP with I-Ani I TS
+3 mCherry
3' iRFP arm
UseLentiviralTagsExpressionBacterial and MammalianMutationembedded I-Ani I TS from 163-185bp, truncated 25 …PromoterSFFVAvailable sinceNov. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
pClneoMyc MOB1 T35A
Plasmid#47937Purposeexpresses Myc tagged human MOB1 containing T35A mutationDepositorInsertMOB1A (MOB1A Human)
UseTagsMycExpressionMammalianMutationT35APromoterCMVAvailable sinceSept. 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
MDH1-miR-324SM-PGK-GFP-Blast
Plasmid#31518DepositorInsertmiR-324 (Mir324 Mouse)
UseRetroviralTagsGFPExpressionMammalianMutationseed mutant controlPromoterAvailable sinceSept. 19, 2011AvailabilityAcademic Institutions and Nonprofits only