Addgene

We narrowed to 14,544 results for: LEA

Showing: 6061 - 6080 of 14544 results

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HOXB1 (human) HIS-tag pET

Plasmid

#8520

Depositor

Corey Largman

Article

Shen et al Nucleic Acids Res 1996 Mar 1;24(5):898-9

Insert

HOXB1 (HOXB1 Human)

Use

Tags

His and T7

Expression

Bacterial

Mutation

PCR from cDNA clone

Promoter

Available since

July 6, 2006

Availability

Academic Institutions and Nonprofits only
p2Flag CMV2-ZO-2-N

Plasmid

#27418

Depositor

Marius Sudol

Article

Oka et al Biochem J. 2010 Nov 25. 432(3):461-72.

Insert

Zonula Occludens 2 (TJP2 Human)

Use

Tags

2xFlag

Expression

Mammalian

Mutation

Deletion of the carboxy-terminus of ZO-2, so only…

Promoter

Available since

April 12, 2011

Availability

Academic Institutions and Nonprofits only
pMKO.1-shCdk1

Plasmid

#160953

Purpose

Cdk1 shRNA in pMKO.1 retroviral vector

Depositor

Matthew Steinhauser

Article

Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

Insert

Cdk1 shRNA (Cdk1 Mouse)

Use

RNAi and Retroviral

Tags

Expression

Mutation

Promoter

U6

Available since

Dec. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pFLAG-4S Tetherin

Plasmid

#41073

Depositor

Paul Spearman

Article

Hammonds et al J Virol. 2012 Feb;86(4):2259-72. Epub 20

Insert

Tetherin (BST2 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

S74, S84, 137S, and 144S

Promoter

CMV

Available since

Oct. 30, 2012

Availability

Academic Institutions and Nonprofits only
API5-CRISPR

Plasmid

#157664

Purpose

Expresses Cas9 and sgRNA targeting API5

Depositor

Byung Il Lee

Article

Bong et al Nucleic Acids Res. 2020 Jun 19;48(11):63

Insert

Apoptosis inhibitor 5 (API5 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 20, 2020

Availability

Academic Institutions and Nonprofits only

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HS-DN-Cep152-mKate2

Plasmid

#105943

Purpose

heat shock inducible transgenesis

Depositor

Caren Norden

Article

Strzyz et al Dev Cell. 2015 Jan 26;32(2):203-19. doi:

Insert

Cep152 (CEP152 Human)

Use

Tags

mKate2

Expression

Bacterial

Mutation

dominant negative (AA 1-512)

Promoter

Available since

Feb. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-α-COPI-WD40

Plasmid

#180758

Purpose

Mammalian expression plasmid for Schizosaccharomyces pombe α-COPI-WD40 (residues 1-327)

Depositor

Syed Saif Hasan

Article

Dey et al Commun Biol. 2022 Feb 8;5(1):115. doi: 1

Insert

α-COPI-WD40

Use

Tags

C-terminal strep tag

Expression

Mammalian

Mutation

Encodes residues 1-327; mutations: Leu181Lys, Leu…

Promoter

CMV

Available since

March 8, 2022

Availability

Academic Institutions and Nonprofits only

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MAP1LC3A-V1

Plasmid

#98569

Purpose

Expresses MAP1LC3A-V1

Depositor

Shahenda El-Naggar

Article

Nassar et al Sci Rep. 2017 Aug 14;7(1):8022. doi: 10.

Insert

MAP1LC3A (MAP1LC3A Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 26, 2017

Availability

Academic Institutions and Nonprofits only

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pFLAG-137.144S Tetherin

Plasmid

#41072

Depositor

Paul Spearman

Article

Hammonds et al J Virol. 2012 Feb;86(4):2259-72. Epub 20

Insert

Tetherin (BST2 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

137S, 144S

Promoter

CMV

Available since

Oct. 30, 2012

Availability

Academic Institutions and Nonprofits only
HOXB3 (human) HIS-tag pET

Plasmid

#8524

Depositor

Corey Largman

Article

Shen et al Nucleic Acids Res 1996 Mar 1;24(5):898-9

Insert

HOXB3 (HOXB3 Human)

Use

Tags

His and T7

Expression

Bacterial

Mutation

Contains 5 additional amino acids before ATG from…

Promoter

Available since

May 30, 2006

Availability

Academic Institutions and Nonprofits only
pWay21-MKP-1L16A/L17A

Plasmid

#13478

Depositor

Anton Bennett

Article

Wu et al Mol Cell Biol. 2005 Jun . 25(11):4792-80

Insert

MKP-1L16A/L17A (Dusp1 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Changed Leucine 16 to Alanine; changed Leucine 1…

Promoter

Available since

Dec. 8, 2006

Availability

Academic Institutions and Nonprofits only
pLV-EF1a-V5-Arl6ip6

Plasmid

#120235

Purpose

Expresses V5-tagged Arl6ip6 in lentiviral vector

Depositor

Larry Gerace

Article

Cheng et al Nucleus. 2019 Dec;10(1):126-143. doi: 10

Insert

Arl6ip6 (Arl6ip6 Mouse)

Use

Lentiviral

Tags

V5

Expression

Mutation

Promoter

EF1a

Available since

Jan. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3 PARL-FLAG-CT H335G

Plasmid

#13749

Depositor

Luca Pellegrini

Article

Cipolat et al Cell. 2006 Jul 14. 126(1):163-75.

Insert

Presenilin-associated rhomboid-like (PARL, PARL Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Changed catalytic His 335 to Gly

Promoter

Available since

Feb. 1, 2007

Availability

Academic Institutions and Nonprofits only
GFP-Drosha218-1374

Plasmid

#62523

Purpose

N-terminal aa1-217 of Drosha is deleted

Depositor

Bharat Ramratnam

Article

Tang et al Nucleic Acids Res. 2010 Oct;38(19):6610-

Insert

human Drosha with aa 1-217 deleted (DROSHA Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

deleted amino acids 1-217

Promoter

Available since

March 20, 2015

Availability

Academic Institutions and Nonprofits only
GFP-Drosha270-1374

Plasmid

#62524

Purpose

N-terminal aa1-269 of Drosha is deleted

Depositor

Bharat Ramratnam

Article

Tang et al Nucleic Acids Res. 2010 Oct;38(19):6610-

Insert

human Drosha with aa 1-269 deleted (DROSHA Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

deleted amino acids 1-269

Promoter

Available since

March 23, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA3-FLAG-Grb10-WT

Plasmid

#74501

Purpose

Overexpression of Grb10 in mammalian cells

Depositor

Lars Rönnstrand

Article

Kazi et al Mol Oncol. 2013 Jun;7(3):402-18. doi: 10

Insert

Grb10 (GRB10 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

L358S, V522A, and V524L

Promoter

CMV

Available since

Dec. 22, 2016

Availability

Academic Institutions and Nonprofits only
pEF Doc2beta 6X mutant GFP

Plasmid

#128823

Purpose

To visualize Doc2beta 6X mutant in PC12 cells

Depositor

Edwin Chapman

Article

Courtney et al Neuron. 2018 Jun 6;98(5):977-991.e5. doi

Insert

Doc2beta (Doc2b Rat)

Use

Tags

EGFP

Expression

Mammalian

Mutation

D163A, D218A, D220N, D303A, D357A, D359A

Promoter

EF-1alpha

Available since

Nov. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pFLAG-74.84S Tetherin

Plasmid

#41071

Depositor

Paul Spearman

Article

Hammonds et al J Virol. 2012 Feb;86(4):2259-72. Epub 20

Insert

Tetherin (BST2 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

74S, 84S

Promoter

CMV

Available since

Oct. 30, 2012

Availability

Academic Institutions and Nonprofits only
3695 pGEX4T-3 MLL AD mut.E

Plasmid

#8867

Depositor

Stanley Korsmeyer

Article

Ernst et al Mol Cell Biol. 2001 Apr . 21(7):2249-58.

Insert

MLL min A.D. (KMT2A Human)

Use

Tags

GST

Expression

Bacterial

Mutation

ILPSDIMDFVL --> ILPADIMDFVL

Promoter

Available since

Aug. 3, 2005

Availability

Academic Institutions and Nonprofits only
pMGF157

Plasmid

#96972

Purpose

ish1-mCherry insertion cassette with Hygro resistance gene

Depositor

Wesley Sundquist, Adam Frost

Article

Gu et al Proc Natl Acad Sci U S A. 2017 Mar 14;11

Insert

ish1 (ish1 Fission Yeast)

Use

Tags

GST and mCherry

Expression

Bacterial

Mutation

ish1 C-terminal domain fused with mCherry + hygro…

Promoter

N/A

Available since

Aug. 11, 2017

Availability

Academic Institutions and Nonprofits only

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