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Plasmid#123105PurposeExpresses 3xFLAG-GABARAPL2 G116 in mammalian cells.DepositorInsertGamma-aminobutyric acid receptor-associated protein-like 2 (GABARAPL2 Human)
UseTags3xFLAGExpressionMammalianMutationDeleted amino acid 117. Stop codon after G116PromoterCMVAvailable sinceMay 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDONR223 PAK5
Plasmid#60532PurposeDonor vector for human Pak5DepositorInsertPak5 (PAK5 Human)
UseGateway donor vectorTagsExpressionMutationPromoterAvailable sinceFeb. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.2/EV
Plasmid#120833PurposeControl vector, 20nt random sequence between gateway recombination sitesDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDN0606 (pDEST-DHFR F[3] N-term,HygR)
Plasmid#210488PurposepDEST vector to tag gene of interest with DHFR F[3] on the N-terminus to perform DHFR-PCA in S. cerevisiae.DepositorTypeEmpty backboneUseSynthetic Biology; Destination vector for gateway…TagsExpressionYeastMutationPromoterADH1Available sinceDec. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKB11 (pDEST-DHFR F[1,2] C-term, NatR)
Plasmid#210485PurposepDEST vector to tag gene of interest with DHFR F[1,2] on the C-terminus to perform DHFR-PCA in S. cerevisiae.DepositorTypeEmpty backboneUseSynthetic Biology; Destination vector for gateway…TagsExpressionYeastMutationPromoterADH1Available sinceDec. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDN0605 (pDEST-DHFR F[1,2] N-term, NatR)
Plasmid#210487PurposepDEST vector to tag gene of interest with DHFR F[1,2] on the N-terminus to perform DHFR-PCA in S. cerevisiae.DepositorTypeEmpty backboneUseSynthetic Biology; Destination vector for gateway…TagsExpressionYeastMutationPromoterADH1Available sinceDec. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMXs-mGLIS1
Plasmid#33273PurposeRetroviral expression of mouse Glis1DepositorInsertGLIS1, Gateway casette A (Glis1 Mouse)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceDec. 14, 2011AvailabilityAcademic Institutions and Nonprofits only -
pYPQ220-Ultra
Plasmid#176889PurposeGateway entry plasmid (attL1 & attR5) expressing AsCas12a with M537R and F870L mutations, without promoterDepositorInsertAsCas12a
UseCRISPR; Gateway compatible cas12a entry cloneTagsNucleoplasmin NLS and SV40 NLSExpressionPlantMutationPromoterAvailable sinceApril 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-MCU
Plasmid#31726DepositorInsertMCU (MCU Human)
UsePdonr gatewayTagsNoneExpressionMutation*PromoterNoneAvailable sinceAug. 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
Lenti-2XPRE-GFP
Plasmid#206169PurposepMVP Gateway destination vector (3rd generation lentiviral vector) containing 2 consensus PGR binding sites upstream of a minimal 2YBTATA promoterDepositorInsert2X PRE-eGFP
UseLentiviralTagsExpressionMutationPromoter2YBTATAAvailable sinceNov. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
tetO-NKX2.5
Plasmid#46029DepositorInsertNKX2.5 (NKX2-5 Human)
UseLentiviralTagsHis and V5ExpressionMammalianMutationPromotertetOAvailable sinceJuly 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDESTN-MTp-p53-Venus
Plasmid#53736PurposeExpresses Venus tagged p53 under the metallothionein promoterDepositorInsertp53 (TP53 Human)
UseTagsVenusExpressionMammalianMutationPromotermetallothionein promoterAvailable sinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDEST-CMV 3xFLAG-GABARAPL1-GFP
Plasmid#123112PurposeExpresses 3xFLAG-GABARAPL1-EGFP in mammalian cells, for monitoring ATG4 activity towards GABARAPL1DepositorInsertGamma-aminobutyric acid receptor-associated protein-like 1 (GABARAPL1 Human)
UseTags3xFLAG and EGFPExpressionMammalianMutationPromoterCMVAvailable sinceMay 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
p6592 MSCV-IP N-HAonly WWP2
Plasmid#34905DepositorInsertWWP2 (WWP2 Human)
UseRetroviralTagsHAExpressionMammalianMutationPromoterMSCV LTRAvailable sinceFeb. 8, 2012AvailabilityAcademic Institutions and Nonprofits only -
pDEST17-His10-ccdB
Plasmid#166145PurposeGateway destination vector for generation of N-terminal His10-tagged proteins in E. coli.DepositorArticleTypeEmpty backboneUseTagsHis10ExpressionBacterialMutationPromoterAvailable sinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
Lenti-6XPRE-GFP
Plasmid#206171PurposepMVP Gateway destination vector (3rd generation lentiviral vector) containing 6 consensus PGR binding sites upstream of a minimal 2YBTATA promoterDepositorInsert6X PRE-eGFP
UseLentiviralTagsExpressionMutationPromoter2YBTATAAvailable sinceNov. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pENTR_L1-Turbo-YFP-NLS-STOP-L2
Plasmid#127361Purposegateway entry vector for expressing nuclear TurboID-YFP under a promoter of choiceDepositorInsertTurboID (BirA mutant)
UseGateway entry vectorTagsGS linker, NLS, V5, and YFPExpressionMutationQ65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…Promoterno promoterAvailable sinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCLX-G4E1b-R1DESTR2-MIFE-PGK-mCherry
Plasmid#114322PurposeMifepristone-inducible lentiviral backbone for Gateway cloning of Gene insert together with constitutive mCherry expressionDepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceSept. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
p3E-Rac1-DN
Plasmid#109582PurposeMultisite gateway vector for 3' tagging with dominant negative Rac1DepositorInsertRac1 (rac1a Zebrafish)
UseZebrafish plasmidsTagsExpressionMutationdominant negativePromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEX-CMV-SP-YFP-STM2(15-746)
Plasmid#18862DepositorInsertStromal interaction molecule 2 (STIM2 Human)
UseTagsYFPExpressionMammalianMutationYFP inserted between signal peptide(SP) of STIM1 …PromoterAvailable sinceMarch 24, 2009AvailabilityAcademic Institutions and Nonprofits only