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pUG36-NES-EGFP-cPHx3

Plasmid

#183668

Purpose

PtdIns(3,4)P2 biosensor for expression in yeast

Depositor

Sergio Grinstein

Article

Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

Insert

PLEKHA1 (PLEKHA1 Frog, Human)

Use

Tags

X. laevis map2k1.L(32-44)-EGFP

Expression

Yeast

Mutation

Amino acids 169-329 (tandem trimer)

Promoter

MET25

Available since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pUG34-NES-EGFP-cPHx3

Plasmid

#183669

Purpose

PtdIns(3,4)P2 biosensor for expression in yeast

Depositor

Sergio Grinstein

Article

Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

Insert

PLEKHA1 (PLEKHA1 Frog, Human)

Use

Tags

X. laevis map2k1.L(32-44)-EGFP

Expression

Yeast

Mutation

Amino acids 169-329 (tandem trimer)

Promoter

MET25

Available since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pESC-LEU-IpgD(C439S)

Plasmid

#183673

Purpose

Inducible Shigella flexneri IpgD (catalytically inactive) for expression in yeast

Depositor

Sergio Grinstein

Article

Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

Insert

IpgD

Use

Tags

Expression

Yeast

Mutation

C439S

Promoter

GAL1

Available since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pNIA-CEN-FLAG-LANS1-Set2

Plasmid

#122002

Purpose

FLAG-LANS-Set2: FLAG-tagged LANS1-Set2 for light control of Set2 localization in yeast

Depositor

Brian Kuhlman

Article

Lerner et al Genome Res. 2020 Nov;30(11):1605-1617. d

Insert

histone methyltransferase SET2 (SET2 Budding Yeast)

Use

Synthetic Biology

Tags

FLAG

Expression

Yeast

Mutation

Set2 point mutations K538G, K539S, R549G, K550S, …

Promoter

ADH1

Available since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

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pGG442

Plasmid

#165616

Purpose

Vector for expression of SpCas9-Zif268 in yeast after assembly with PID fragments: TEF1p-SpCas9::KanR-1xNLS-3xHA-1xNLS-Zif268-1xNLS-CYC1t (KanR cassette in place of the PID)

Depositor

Marcus Noyes

Article

Goldberg et al Nat Commun. 2021 Jan 13;12(1):349. doi:

Insert

TEF1p-SpCas9::KanR(in place of PID)-1xNLS-3xHA-1xNLS-Zif268-1xNLS-CYC1t

Use

CRISPR

Tags

3x HA, SV40 NLS, Zif268, and c-Myc-like NLS

Expression

Yeast

Mutation

Corrected homology relative to wild type SpCas9 (…

Promoter

TEF1

Available since

April 21, 2021

Availability

Academic Institutions and Nonprofits only

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pRS313_ATP3G919C

Plasmid

#120255

Purpose

Expression of ATP3-7 in yeast

Depositor

Markus Ralser

Article

Vowinckel et al Nat Metab. 2021 Nov;3(11):1521-1535. doi

Insert

ATP3 (ATP3 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

G919 changed to C; Alanine 307 changed to Proline

Promoter

ATP3

Available since

April 8, 2021

Availability

Academic Institutions and Nonprofits only

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pRS313_ATP3mut

Plasmid

#120256

Purpose

Expression of ATP3-67 in yeast

Depositor

Markus Ralser

Article

Vowinckel et al Nat Metab. 2021 Nov;3(11):1521-1535. doi

Insert

ATP3 (ATP3 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

Isoleucine 304 changed to Asparagine; Alanine 307…

Promoter

ATP3

Available since

April 8, 2021

Availability

Academic Institutions and Nonprofits only

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CPH3568 (YCp LEU2 Rpb1 P1455_E1456InsGGGGGLEVLFQGP(H)10,N1501K; msLink1)

Plasmid

#91819

Purpose

Yeast expression of S. cerevisiae Rpb1 with an internal PreScission protease site and 10xHis tag for purification of the linker-CTD

Depositor

Chris Hill

Article

Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

Insert

RPO21 (RPO21 Budding Yeast)

Use

Tags

10xHis

Expression

Yeast

Mutation

5xGly linker, PreScission Protease site (LEVLFQGP…

Promoter

Endogenous Rpb1 promoter

Available since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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CPH3569 (YCp LEU2 Rpb1 P1455_E1456InsGGGGGLEVLFQGP(H)10,K1487N,D1497K; msLink2)

Plasmid

#91820

Purpose

Yeast expression of S. cerevisiae Rpb1 with an internal PreScission protease site and 10xHis tag for purification of the linker-CTD

Depositor

Chris Hill

Article

Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

Insert

RPO21 (RPO21 Budding Yeast)

Use

Tags

10xHis

Expression

Yeast

Mutation

5xGly linker, PreScission Protease site (LEVLFQGP…

Promoter

Endogenous Rpb1 promoter

Available since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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DHC1-mIAA7 Neo

Plasmid

#140543

Purpose

DHC1 tagging with mIAA7

Depositor

Masato Kanemaki

Article

Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

Insert

DHC1 (DYNC1H1 Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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DHC1-mIAA17 Hygro

Plasmid

#140544

Purpose

DHC1 tagging with mIAA7

Depositor

Masato Kanemaki

Article

Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

Insert

DHC1 (DYNC1H1 Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pPICZ-aC-MSVU-BZ

Plasmid

#160426

Purpose

Expression of c-luciferase from sp. Maristella sp. SVU (Belize)

Depositor

Todd Oakley

Article

Hensley et al Mol Ecol. 2020 Oct 8. doi: 10.1111/mec.1

Insert

luciferase

Use

Luciferase

Tags

6x Histidine tag, Yeast alpha secretion factor, c…

Expression

Yeast

Mutation

See depositor comments below

Promoter

AOX

Available since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pDUAL-Pnmt1-Ost4-GFP

Plasmid

#154368

Purpose

Yeast expression of Ost4

Depositor

Wanzhong He

Article

Jiang et al Nat Methods. 2020 Sep;17(9):937-946. doi

Insert

Ost4 (ost4 Fission Yeast)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pDUAL-P41nmt1-Sad1-GFP

Plasmid

#154366

Purpose

Yeast expression of Sad1

Depositor

Wanzhong He

Article

Jiang et al Nat Methods. 2020 Sep;17(9):937-946. doi

Insert

Sad1 (sad1 Fission Yeast)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

July 24, 2020

Availability

Academic Institutions and Nonprofits only

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pDUAL-P41nmt1-Nup124-GFP

Plasmid

#154364

Purpose

Yeast expression of Nup124

Depositor

Wanzhong He

Article

Jiang et al Nat Methods. 2020 Sep;17(9):937-946. doi

Insert

Nup124 (nup124 Fission Yeast)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

July 23, 2020

Availability

Academic Institutions and Nonprofits only

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pDUAL-Pnmt1-Ost4-GFP-MTalpha

Plasmid

#154370

Purpose

Yeast expression of Ost4

Depositor

Wanzhong He

Article

Jiang et al Nat Methods. 2020 Sep;17(9):937-946. doi

Insert

Ost4 (ost4 Fission Yeast)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

July 23, 2020

Availability

Academic Institutions and Nonprofits only

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pDUAL-P41nmt1-Sad1-GFP-MTn

Plasmid

#154367

Purpose

Yeast expression of Sad1

Depositor

Wanzhong He

Article

Jiang et al Nat Methods. 2020 Sep;17(9):937-946. doi

Insert

Sad1 (sad1 Fission Yeast)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

July 23, 2020

Availability

Academic Institutions and Nonprofits only

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pTH748-CEN-RLuc/min8maxCFLuc

Plasmid

#38214

Depositor

Tobias von der Haar

Article

Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

Inserts

Firefly Luciferase
Renilla luciferase

Use

Tags

Expression

Yeast

Mutation

The first eight codons of the original FLuc gene …

Promoter

ADH1 and TDH3 (=GDP)

Available since

Nov. 2, 2012

Availability

Academic Institutions and Nonprofits only

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pTH731-2µ-RLuc/minCFLuc

Plasmid

#40601

Depositor

Tobias von der Haar

Article

Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

Inserts

Firefly Luciferase
Renilla luciferase

Use

Tags

Expression

Yeast

Mutation

All codons of the original FLuc gene were exchang…

Promoter

ADH1 and TDH3

Available since

Oct. 30, 2012

Availability

Academic Institutions and Nonprofits only

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pTH753-CEN-RLuc/min346maxCFLuc

Plasmid

#38219

Depositor

Tobias von der Haar

Article

Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

Inserts

Firefly Luciferase
Renilla luciferase

Use

Tags

Expression

Yeast

Mutation

The first 346 codons of the original FLuc gene we…

Promoter

ADH1 and TDH3 (=GPD)

Available since

Oct. 22, 2012

Availability

Academic Institutions and Nonprofits only

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