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Plasmid#90990PurposeNAT-marked C. albicans ADE2-specific gRNA expression construct; part 2 of 2 of C.mal LEUpOUT CRISPR system. Use with pADH140 CAS9 expression construct.DepositorInsertNAT 2 of 2, pSNR52, ADE2 gRNA, gRNA conserved, C. maltosa LEU2 2 of 2
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJune 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pADH100-2
Plasmid#90981PurposeNAT-marked C. albicans ADE2-specific gRNA expression construct; part 2 of 2 of C.alb HIS1-FLP CRISPR system. Use with pADH99 CAS9 expression construct.DepositorInsertNAT 2 of 2, pSNR52, ADE2 gRNA, gRNA conserved, FRT, DS_HIS1
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJune 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLK88 - Edit-directing plasmid base
Plasmid#98814PurposeEdit-directing plasmid baseDepositorInsertCAN1.y gRNA
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceJuly 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPN10-gCTG
Plasmid#114385PurposepPN10 with gRNA for spCRISPR-Cas9 targeted to CTG repeat, PAM: CTGDepositorInsertgCTG
UseCRISPRTagsExpressionMutationPromoterAvailable sinceMarch 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPN10-gCAG
Plasmid#114384PurposepPN10 with gRNA for spCRISPR-Cas9 targeted to CAG repeat, PAM: CAGDepositorInsertgCAG
UseCRISPRTagsExpressionMutationPromoterAvailable sinceMarch 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRB1081
Plasmid#71479PurposeExpresses gRNA for dpy-10 conversion using NGAG PAM and VQR Cas9 variantDepositorInsertdpy-10 gRNA
UseCRISPRTagsExpressionWormMutationPromoterU6Available sinceDec. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAH233
Plasmid#121144PurposeExpression of gRNA with BbsI placeholder for target cloningDepositorInsertgRNA cassette
UseCRISPRTagsExpressionYeastMutationPromoterS.pombe rrk1Available sinceFeb. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRB1084
Plasmid#71516PurposeExpresses gRNA for dpy-10 conversion using NGCG PAM and VRER Cas9 mutantDepositorInsertdpy-10 gRNA
UseCRISPRTagsExpressionWormMutationPromoterU6Available sinceDec. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
PX458_ZNF219_1
Plasmid#72368PurposeEncodes gRNA for 3' target of human ZNF219 along with Cas9 with 2A GFPDepositorInsertZNF219 (ZNF219 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceFeb. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
PX458_ZNF219_2
Plasmid#72369PurposeEncodes gRNA for 3' target of human ZNF219 along with Cas9 with 2A GFPDepositorInsertZNF219 (ZNF219 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceFeb. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
PX458_TGIF2_1
Plasmid#72374PurposeEncodes gRNA for 3' target of human TGIF2 along with Cas9 with 2A GFPDepositorInsertTGIF2 (TGIF2 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceFeb. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
PX458_TGIF2_2
Plasmid#72375PurposeEncodes gRNA for 3' target of human TGIF2 along with Cas9 with 2A GFPDepositorInsertTGIF2 (TGIF2 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceFeb. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
PX458_ZNF146_1
Plasmid#72360PurposeEncodes gRNA for 3' target of human ZNF146 along with Cas9 with 2A GFPDepositorInsertZNF146 (ZNF146 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceFeb. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
PX458_ZNF146_2
Plasmid#72361PurposeEncodes gRNA for 3' target of human ZNF146 along with Cas9 with 2A GFPDepositorInsertZNF146 (ZNF146 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceFeb. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
STAgR_Neo
Plasmid#102992PurposeCan be used as backbone for a STAgR reactionDepositorTypeEmpty backboneUseCRISPR; Pcr template for stagr vectorsTagsExpressionMutationPromoterU6Available sinceFeb. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLdCN
Plasmid#84290PurposeExpress gRNA and Cas9 in Leishmania with Neomycin resistanceDepositorInsertgRNA and Cas9
UseCRISPR; LeishmaniaTagsExpressionMutationPromoterL. donovani ribosome RNA promoterAvailable sinceJan. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
STAgR_tdTomato
Plasmid#102993PurposeCan be used as backbone for a STAgR reactionDepositorTypeEmpty backboneUseCRISPR; Pcr template for stagr vectorsTagsExpressionMutationPromoterU6Available sinceMarch 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEasyG1_hph
Plasmid#184912PurposeTemplate to generate via PCR a single gRNA for expression in S. cerevisiaeDepositorInsertgRNA scaffold
UseCRISPRTagsExpressionBacterial and YeastMutationPromoterAvailable sinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pLdCH
Plasmid#84291PurposeExpress gRNA and Cas9 in Leishmania with Hygromycin resistanceDepositorInsertgRNA and Cas9
UseCRISPR; LeishmaniaTagsExpressionMutationPromoterL. donovani ribosome RNA promoterAvailable sinceJan. 30, 2017AvailabilityAcademic Institutions and Nonprofits only