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pRevTRE-hygro-GFP-Crumbs3a WT

Plasmid

#195217

Purpose

Retroviral Tet response vector containing GFP tagged Crumbs3a

Depositor

David Bryant, Ben Margolis

Article

Schluter et al Mol Biol Cell. 2009 Nov;20(22):4652-63.

Insert

CRB3A (CRB3 Human)

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

March 2, 2023

Availability

Academic Institutions and Nonprofits only

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siRNA-resistant ABHD16A-H189AD194A-Halo

Plasmid

#195168

Purpose

mammalian expression of siRNA-resistant mutant ABHD16A tagged with Halo tag

Depositor

Gia Voeltz

Article

Nguyen et al Elife. 2022 Nov 30;11:e84279. doi: 10.75

Insert

ABHD16A (ABHD16A Human)

Use

Tags

Halo tag

Expression

Mammalian

Mutation

Wobble mutations for siRNA resistance from L90 to…

Promoter

CMV

Available since

Feb. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV6-ZNF354C_mutant_D17A-V18A

Plasmid

#194184

Purpose

Expresses ZNF354C with mutated KRAB domain (D17A, V18A mutation)

Depositor

Matthias Leisegang, Ralf Brandes

Article

Oo et al Sci Rep. 2020 Nov 5;10(1):19079. doi: 10

Insert

ZNF354C (ZNF354C Human)

Use

Tags

Myc-DDK

Expression

Mammalian

Mutation

KRAB domain mutant D17A/V18A

Promoter

CMV

Available since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV6-ZNF354C_mutant_E25A-W26A

Plasmid

#194186

Purpose

Expresses ZNF354C with mutated KRAB domain (E25A, W26A mutation)

Depositor

Matthias Leisegang, Ralf Brandes

Article

Oo et al Sci Rep. 2020 Nov 5;10(1):19079. doi: 10

Insert

ZNF354C (ZNF354C Human)

Use

Tags

Myc-DDK

Expression

Mammalian

Mutation

KRAB domain mutant E25A/W26A

Promoter

CMV

Available since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pS238D1::tse5-CT

Plasmid

#192958

Purpose

For expression of Tse5-CT (1169-1317) in P. putida for biological function studies

Depositor

David Albesa-Jové

Article

Gonzalez-Magana et al Commun Biol. 2022 Nov 5;5(1):1189. doi:

Insert

tse5-CT

Use

Tags

Expression

Bacterial

Mutation

encodes for tse5 (1169-1317)

Promoter

Available since

Jan. 19, 2023

Availability

Academic Institutions and Nonprofits only

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H1.99.luc

Plasmid

#193663

Purpose

Expresses a truncated Polymerase III H1 promoter of 99 nucleotides (H1-99)

Depositor

Elena Herrera-Carrillo

Article

Gao et al J Biol Chem. 2020 Nov 5. pii: S0021-9258

Insert

Truncated Pol III H1 promoter (H1.99) + 41 nucleotides

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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H1.99.M.luc

Plasmid

#193664

Purpose

Expresses a truncated Polymerase III H1 promoter of 99 nucleotides (H1-99) with the TATA box mutated

Depositor

Elena Herrera-Carrillo

Article

Gao et al J Biol Chem. 2020 Nov 5. pii: S0021-9258

Insert

Truncated Pol III H1 promoter with a mutated TATA box (H1.99m) + N41

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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H1.224.M.luc

Plasmid

#193705

Purpose

Expresses a truncated PolIII H1 promoter of 224 nucleotides (H1.224) with the TATA box mutated

Depositor

Elena Herrera-Carrillo

Article

Gao et al J Biol Chem. 2020 Nov 5. pii: S0021-9258

Insert

Truncated PolIII H1 promoter (H1.224) with the TATA box mutated

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pRD424

Plasmid

#178202

Purpose

Expression of Rok from the hns promoter

Depositor

Remus Dame

Article

Erkelens et al Nucleic Acids Res. 2022 Nov 21. pii: 683

Insert

phns + rok

Use

Tags

Expression

Bacterial

Mutation

Promoter

hns promoter

Available since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pJH4143

Plasmid

#191531

Purpose

Punc-17-Cre unc-54 3'UTR C.elegans for Cre/Lox

Depositor

Mei Zhen

Article

Lu et al Curr Biol. 2022 Nov 7;32(21):4631-4644.e

Insert

Cre

Use

Cre/Lox

Tags

no taq

Expression

Mutation

Promoter

Punc-17

Available since

Dec. 13, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pRD410

Plasmid

#178200

Purpose

Expression of sRok from the hns promoter

Depositor

Remus Dame

Article

Erkelens et al Nucleic Acids Res. 2022 Nov 21. pii: 683

Insert

phns + srok

Use

Tags

Expression

Bacterial

Mutation

Promoter

hns promoter

Available since

Nov. 22, 2022

Availability

Academic Institutions and Nonprofits only

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EGFP-Ki-67(RASA)-mCherry_IRESpuro2

Plasmid

#182921

Purpose

Expresses EGFP-Ki-67(RASA)-mCherry. Ki67 contains RASA mutation.

Depositor

Daniel Gerlich

Article

Cuylen-Haering et al Nature. 2020 Nov;587(7833):285-290. doi:

Insert

MKI67 (MKI67 Human)

Use

Tags

EGFP and mCherry

Expression

Bacterial and Mammalian

Mutation

RASA mutation

Promoter

CMV

Available since

Sept. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pIALD2HMGr

Plasmid

#185867

Purpose

Knocking out ALD6; expressing acetylating aldehyde dehydrogenase (Lactobacillus reuteri pduP and L. reuteri EutE) and an NADH-preferring HMG-CoA reductase (Delftia acidovorans mvaA) in S. cerevisiae

Depositor

Claudia Vickers

Article

Hayat et al Microb Biotechnol. 2021 Nov;14(6):2627-2

Insert

ALD6(-125, 40)> PSk.GAL1>Da.mvaA>TPGK1-PADH1> Lr.pduP> TPDC1-PGAL2>Lr.EutE-ALD6(1054, 1749)

Use

Tags

Expression

Yeast

Mutation

None

Promoter

Available since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

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KBB430

Plasmid

#185091

Purpose

NMA111-GFP with first nuclear localization signal mutated (amino acids 28 - 30, KRK -> AAA)

Depositor

Ken Belanger

Article

Belanger et al J Cell Sci. 2009 Nov 1;122(Pt 21):3931-4

Insert

NMA111

Use

Tags

GFP

Expression

Yeast

Mutation

First nuclear localization signal mutated (amino …

Promoter

Available since

July 29, 2022

Availability

Academic Institutions and Nonprofits only

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KBB428

Plasmid

#185090

Purpose

NMA111-GFP with first nuclear localization signal mutated (amino acids 9 - 11, KKR -> AAA)

Depositor

Ken Belanger

Article

Belanger et al J Cell Sci. 2009 Nov 1;122(Pt 21):3931-4

Insert

NMA111

Use

Tags

GFP

Expression

Yeast

Mutation

First nuclear localization signal mutated (amino …

Promoter

Available since

July 27, 2022

Availability

Academic Institutions and Nonprofits only

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H2B-mNeonGreen_FRB(DmrC)_IRESpuro2

Plasmid

#182918

Purpose

Expesses H2B-mNeonGreen_FRB(DmrC). Can be used to target FKBP(DmrA) tagged proteins to H2B

Depositor

Daniel Gerlich

Article

Cuylen-Haering et al Nature. 2020 Nov;587(7833):285-290. doi:

Insert

pH2B (H2BC16P Human)

Use

Tags

mNeonGreen-FRB(DmrC)

Expression

Bacterial and Mammalian

Mutation

Promoter

CMV

Available since

July 27, 2022

Availability

Academic Institutions and Nonprofits only

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pTW037

Plasmid

#185688

Purpose

T-DNA for creating transgenic plants expressing Cas9 and Drm1b gRNAs

Depositor

Feng Zhang

Article

Weiss et al Plant J. 2020 Nov;104(3):828-838. doi: 1

Insert

Cas9, Drm1b gRNA

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

July 19, 2022

Availability

Academic Institutions and Nonprofits only

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MSCV-Arkadia C946S H948L

Plasmid

#180373

Purpose

expressing mouse Arkadia (isoform 3) C946S H948L E3 ligase dead mutant proteins

Depositor

Dan Littman

Article

Xu et al J Exp Med. 2021 Nov 1;218(11). pii: 2126

Insert

Arkadia (Rnf111 Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

C946S H948L

Promoter

LTR promoter

Available since

June 14, 2022

Availability

Academic Institutions and Nonprofits only

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pSIN-Skil-gRNA1

Plasmid

#180370

Purpose

targeting mouse Skil/SnoN gene

Depositor

Dan Littman

Article

Xu et al J Exp Med. 2021 Nov 1;218(11). pii: 2126

Insert

Skil targeting gRNA (Skil Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

human U6

Available since

June 10, 2022

Availability

Academic Institutions and Nonprofits only

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pSIN-Ski-gRNA1

Plasmid

#180368

Purpose

targeting mouse Ski gene

Depositor

Dan Littman

Article

Xu et al J Exp Med. 2021 Nov 1;218(11). pii: 2126

Insert

Ski targeting gRNA (Ski Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

human U6

Available since

June 10, 2022

Availability

Academic Institutions and Nonprofits only

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