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Plasmid#99848PurposeExpresses Cre-recombinase, a barcoded HDR template that serves as a control for HDR into the endogenous Kras locus, and a Kras-targeting sgRNA to enhance HDR.DepositorInsertKras (Kras Mouse)
UseAAV and Cre/LoxTagsExpressionMammalianMutationAvrII site (CAAAGG>CCTAGG), PAM/sgRNA mutation…PromoterAvailable sinceDec. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMS81(rpl-28p::mKate2::unc-54 3'UTR::LoxP::rps-0p::HYGR∆)
Plasmid#154840PurposeInsertion of rpl-28p::mKate2::unc-54 3'UTR into a split hygromycin landing pad. Can be modified to insert a different gene(s) of interest.DepositorInserthomology arm:rpl-28p::mKate2::unc-54 3'UTR::LoxP::rps-0p::HYGR∆
UseCRISPR and Cre/LoxTagsExpressionWormMutationHYGR∆ encodes aa 1-226PromoterAvailable sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKHR5
Plasmid#74593Purposean alpha-crystallin::Venus (green lens) reporter cassette flanked by FRT sites and with a single loxP site at the 3’ end was inserted in the middle of pKHR4 mcs. Genbank accession number is KU144823.DepositorInsertFRT-alpha-crystallin::Venus-FRT-loxP reporter cassette
UseCre/Lox; Zebrafish expressionTagsExpressionMutationPromoteralpha-crystallinAvailable sinceApril 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHA1
Plasmid#85709PurposeThis plasmid provides a BAC vector backbone, which can be used for cloning of BAC inserts, for instance, herpesvirus genomesDepositorTypeEmpty backboneUseCre/Lox; Herpesvirus cloningTagsExpressionBacterialMutationPromoterAvailable sinceJune 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEF1α-Cre-2A-miRFP670nano3
Plasmid#207747PurposeExpression of the Cre recombinase linked to a miRFP670nano3 fluorescent markerDepositorArticleInsertCre-PGK-miRFP670nano3
UseCre/LoxTagsExpressionMammalianMutationPromoterEF1alphaAvailable sinceNov. 15, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
paavCAG-JxOFF-pre-mGRASP-mCerulean
Plasmid#51900PurposeJxOFF-preDepositorInsertpresynaptic mGRASP
UseAAV and Cre/LoxTagsExpressionMutationPromoterAvailable sinceApril 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-FLEX-GT
Plasmid#26198DepositorInsertEnhanced green fluorescent protein, TVA (eGFP Mouse, TVA is a mouse codon optimized version of an avian gene.)
UseAAV and Cre/LoxTagsExpressionMutationTVA mammalian codon optimizedPromoterAvailable sinceSept. 16, 2010AvailabilityAcademic Institutions and Nonprofits only -
PB CAG-F-intron-L-F-bpA-SV40pA-L-td-sfGFP
Plasmid#178433PurposeCAG tdGFP FLP/FRT cre/lox "NOT" reporter piggyBac transposon (bpA SV40pA stop)DepositorInserttd-sfGFP
UseCre/Lox; Flp/frt, piggybacTagsExpressionMammalianMutationPromoterAvailable sinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
CAG-loxP-3xpA-loxP-tdTomato+RbG and HsbG syntrons
Plasmid#172479PurposetdTomato with synthetic introns derived from the rabbit beta-globin gene and the human beta-globin geneDepositorInserttdTomato
UseCre/LoxTagsExpressionMammalianMutationPromoterAvailable sinceAug. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEF1α-Cre-2A-mScarlet
Plasmid#207745PurposeExpression of the Cre recombinase linked to a mScarlet fluorescent markerDepositorArticleInsertCre-PGK-mScarlet
UseCre/LoxTagsExpressionMammalianMutationPromoterEF1alphaAvailable sinceNov. 15, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAAV-DIO-mDLX-TVA-2A-N2cG
Plasmid#172365PurposeTargeting envA-pseodotyped RVdG-CVS-N2c vectors for specific retrograde labeling from cre-expressing inhibitory neuronsDepositorInsertTVA + B19-N2cG
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromotermDLXAvailable sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLP-PIGA2 (pLP300)
Plasmid#168462PurposeBig-IN Landing Pad plasmid encoding pEF1a-driven PuroR-P2A-hmPIGA-P2A-mScarlet expression cassette flanked by heterotypic LoxM/LoxP sites and BsaI homology arms cloning sites.DepositorTypeEmpty backboneUseCre/Lox, Mouse Targeting, and Synthetic Biology ;…TagsExpressionMammalianMutationPromoterEF1aAvailable sinceMay 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEZ-Frt-lox-DT
Plasmid#11736DepositorInsertpEZ-Frt-lox-DT
UseCre/Lox; /frtTagsExpressionMutationPromoterAvailable sinceMay 17, 2006AvailabilityAcademic Institutions and Nonprofits only -
pSL1180-HR-Aag2-loxP-PUb-RFP-loxP-3xFLAG-AGO1
Plasmid#162164PurposeSelectable homology donor template for Aag2 cell AGO1DepositorInsertsRFP
3xFLAG
UseCRISPR and Cre/Lox; Homology donor templateTagsAGO1 homology and loxPExpressionInsectMutationPromoterAe. aegypti PUb and endogenous AGO1Available sinceJan. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
p221-RV-dlx-dio-Tdtomato
Plasmid#185697PurposeRetroviral vector expression cre-dependent membrane tagged Tdtomato driven by the mDlx promoterDepositorInsertmembrane Tdtomato
UseCre/Lox and RetroviralTagsGap43 palmitoylationExpressionMutationPromotermDlxAvailable sinceJuly 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
Pdx1 Cre-ER (DM#255)
Plasmid#15022DepositorInsertPdx1 promoter (Pdx1 Mouse)
UseCre/Lox; Mouse transgenicTagsCre-ER(TM)ExpressionMammalianMutationPromoterAvailable sinceOct. 18, 2007AvailabilityAcademic Institutions and Nonprofits only -
pUC118-mCtnnb1-FLIP-Puro
Plasmid#84540PurposeCRISPR - FLIP targeting vector for mouse beta-cateninDepositorInsertmouse beta-catenin FLIP - Puro targeting vector
UseCre/Lox and Mouse TargetingTagsExpressionMutationPromoterAvailable sinceJan. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
CAG-loxP-3xpA-loxP-H2B-sfGFP
Plasmid#136064PurposeH2B-sfGFP cre reporterDepositorInsertHIST1H2BB (H2BC3 Human)
UseCre/LoxTagssfGFPExpressionMammalianMutationPromoterAvailable sinceMarch 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-FLEX-ArchT-GFP
Plasmid#58851PurposeAAV-mediated expression of ArchT-GFP under the EF1a promoter, in floxed/reversed (Cre-dependent) mannerDepositorInsertArchT-GFP
UseAAV and Cre/LoxTagsGFPExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
p164-pAAV-mDlx-FLEX-mScarlet
Plasmid#204689PurposeFluorescent reporter under the control of the Dlx promotorDepositorInsertmScarlet
UseAAV and Cre/LoxTagsmScarlet palmitoylationExpressionMammalianMutationPromotermDlxAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only