Addgene

We narrowed to 164 results for: pGEX-4T-1

Showing: 41 - 60 of 164 results

Results per page:

Sort results by:

pGEX-4T-1 mSesn1 (1189)

Plasmid

#66917

Purpose

bacterial expression of mouse Sesn1

Depositor

Michael Karin, Jun Hee Lee

Article

Lee et al Cell Metab. 2012 Sep 5;16(3):311-21. doi

Insert

Sesn1 (Sesn1 Mouse)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

tac

Available since

Dec. 18, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX-4T-1 mSesn2 (1190)

Plasmid

#66916

Purpose

bacterial expression of mouse Sesn2

Depositor

Michael Karin, Jun Hee Lee

Article

Park et al Nat Commun. 2014 Jun 20;5:4233. doi: 10.

Insert

Sesn2 (Sesn2 Mouse)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

tac

Available since

Dec. 18, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX-4T-3-SpSuc1

Plasmid

#162558

Purpose

Expresses GST-tagged S.pombe Suc1 in E.coli

Depositor

Iain Hagan

Article

Singh et al Methods Mol Biol. 2021;2329:123-142. doi

Insert

Schizosaccharomyces pombe Suc1 (suc1 Fission Yeast)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 29, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pGex-4T-1 Jip4 (1-272)

Plasmid

#59002

Purpose

JIP4 serves as an activator of the p38 mitogen-activated protein (MAP) kinase pathway by a mechanism that requires the MAP kinase kinases MKK3 and MKK6

Depositor

Roger Davis

Article

Kelkar et al Mol Cell Biol. 2005 Apr;25(7):2733-43.

Insert

Mapk8ip4 (Spag9 Mouse)

Use

Tags

Gst

Expression

Bacterial

Mutation

Jip4 (aa1-272)

Promoter

Available since

Aug. 21, 2014

Availability

Academic Institutions and Nonprofits only
pGex-4T-2_GATE-16

Plasmid

#73518

Purpose

For recombinant expression of human GATE-16/GABARAPL2 in E. coli

Depositor

Dieter Willbold

Article

Ma et al J Biol Chem. 2013 Dec 27;288(52):37204-1

Insert

GATE-16 (GABARAPL2 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

tac

Available since

March 25, 2016

Availability

Academic Institutions and Nonprofits only
pGEX-4T-1 3xHA-MEK1-DD

Plasmid

#47576

Purpose

Bacterial expression plasmid for GST-3xHA-tagged constitutively active MEK1

Depositor

Kevin Janes

Article

Bose et al Mol Cell Proteomics. 2013 Mar;12(3):797-

Insert

MEK1-DD (Map2k1 Mouse)

Use

Tags

3xHA and GST

Expression

Bacterial

Mutation

Ser218 to Asp, Ser221 to Asp

Promoter

Available since

Aug. 28, 2013

Availability

Academic Institutions and Nonprofits only
pGEX-4T-1-β4 hinge-ear

Plasmid

#196692

Purpose

Expresses GST-AP-4 β4 hinge-ear (residues 534-739) in bacteria

Depositor

Juan Bonifacino

Article

Mattera et al J Biol Chem. 2015 Dec 25;290(52):30736-4

Insert

AP-4 β4 hinge-ear (534-739) (AP4B1 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

tac

Available since

March 9, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX-4T-1 EGFP-Sumo2 GG

Plasmid

#13384

Depositor

Mary Dasso

Article

Azuma et al J Cell Biol. 2003 Nov 10. 163(3):477-87.

Insert

Sumo2 GG (SUMO2 Human)

Use

Tags

EGFP and GST

Expression

Bacterial

Mutation

Mature SUMO2GG: diglycine at amino acid positions…

Promoter

Available since

Nov. 16, 2006

Availability

Academic Institutions and Nonprofits only
pGEX-4T-1-p19-T7-Vif

Plasmid

#46313

Depositor

Judy Lieberman

Article

Huang et al Nat Biotechnol. 2013 Apr;31(4):350-6. do

Inserts

p19
Vif DNA-1
linker
Vif DNA-2

Use

Pro-sirna

Tags

6XHis and GST

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 2, 2014

Availability

Academic Institutions and Nonprofits only
pGEX-4T-1 3xHA-MKK4-EE

Plasmid

#47577

Purpose

Bacterial expression plasmid for GST-3xHA-tagged constitutively active MKK4

Depositor

Kevin Janes

Article

Bose et al Mol Cell Proteomics. 2013 Mar;12(3):797-

Insert

MKK4-EE (MAP2K4 Human)

Use

Tags

3xHA and GST

Expression

Bacterial

Mutation

Lacks the first 36 residues of the RefSeq protein…

Promoter

Available since

Aug. 28, 2013

Availability

Academic Institutions and Nonprofits only
pGEX-4T-1 3xHA-MKK6-EE

Plasmid

#47579

Purpose

Bacterial expression plasmid for GST-3xHA-tagged constitutively active MKK6

Depositor

Kevin Janes

Article

Bose et al Mol Cell Proteomics. 2013 Mar;12(3):797-

Insert

MKK6-EE (MAP2K6 Human)

Use

Tags

3xHA and GST

Expression

Bacterial

Mutation

Ser207 to Glu, Thr211 to Glu

Promoter

Available since

Aug. 28, 2013

Availability

Academic Institutions and Nonprofits only
pGEX-4T-1-p19-T7-PLK1

Plasmid

#46312

Depositor

Judy Lieberman

Article

Huang et al Nat Biotechnol. 2013 Apr;31(4):350-6. do

Inserts

p19
PLK1 DNA-1
linker
PLK1 DNA-2

Use

Pro-sirna

Tags

6XHis and GST

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 2, 2014

Availability

Academic Institutions and Nonprofits only
pGEX-4T-1-p19-T7-EGFP100

Plasmid

#46309

Depositor

Judy Lieberman

Article

Huang et al Nat Biotechnol. 2013 Apr;31(4):350-6. do

Inserts

p19
EGFP100 DNA-1
linker
EGFP100 DNA-2

Use

Pro-sirna

Tags

6XHis and GST

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 2, 2014

Availability

Academic Institutions and Nonprofits only
pGEX-4T-1 3xHA-MKK7a1-EE

Plasmid

#47578

Purpose

Bacterial expression plasmid for GST-3xHA-tagged constitutively active MKK7a1

Depositor

Kevin Janes

Article

Bose et al Mol Cell Proteomics. 2013 Mar;12(3):797-

Insert

MKK7a1-EE (Map2k7 Mouse)

Use

Tags

3xHA and GST

Expression

Bacterial

Mutation

Residues 73-420 of RefSeq sequence. Ser271 to Gl…

Promoter

Available since

Aug. 28, 2013

Availability

Academic Institutions and Nonprofits only
pGEX-4T-1-p19-T7-EGFPFL

Plasmid

#46310

Depositor

Judy Lieberman

Article

Huang et al Nat Biotechnol. 2013 Apr;31(4):350-6. do

Inserts

p19
EGFPFL DNA-1
linker
EGFPFL DNA-2

Use

Pro-sirna

Tags

6XHis and GST

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 2, 2014

Availability

Academic Institutions and Nonprofits only
pGEX-4T-1 EGFP-Sumo2 G

Plasmid

#13383

Depositor

Mary Dasso

Article

Azuma et al J Cell Biol. 2003 Nov 10. 163(3):477-87.

Insert

Sumo2 G (SUMO2 Human)

Use

Tags

EGFP and GST

Expression

Bacterial

Mutation

Nonconjugatable SUMO2G: single C-terminal glycine…

Promoter

Available since

Nov. 16, 2006

Availability

Academic Institutions and Nonprofits only
pGEX-4T-1-p19-T7-GagB500

Plasmid

#46314

Depositor

Judy Lieberman

Article

Huang et al Nat Biotechnol. 2013 Apr;31(4):350-6. do

Inserts

p19
GagB500 DNA-1
linker
GagB500 DNA-2

Use

Pro-sirna

Tags

6XHis and GST

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 2, 2014

Availability

Academic Institutions and Nonprofits only
pGEX-4T-1-Rnt1-dsRBD(+6A)

Plasmid

#171552

Purpose

Encoding part of the budding yeast Rnt1 protein from L366 to S453

Depositor

Yan Zeng

Article

Zhang et al Acta Biochim Biophys Sin (Shanghai). 202

Insert

Rnt1 (RNT1 Budding Yeast)

Use

Tags

Expression

Bacterial

Mutation

a six-alanine insertion in the L1 region

Promoter

Available since

Dec. 8, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX-4T-1-Rnt1-dsRBD(+3A)

Plasmid

#171551

Purpose

Encoding part of the budding yeast Rnt1 protein from L366 to S453

Depositor

Yan Zeng

Article

Zhang et al Acta Biochim Biophys Sin (Shanghai). 202

Insert

Rnt1 (RNT1 Budding Yeast)

Use

Tags

Expression

Bacterial

Mutation

a three-alanine insertion in the L1 region

Promoter

Available since

Aug. 12, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX-4T-1-Xlrbpa-dsRBD(+6)

Plasmid

#171548

Purpose

Encoding part of the Xenopus Xlrbpa-2 protein from M120 to L202

Depositor

Yan Zeng

Article

Zhang et al Acta Biochim Biophys Sin (Shanghai). 202

Insert

Xlrbpa (prkra.L Frog)

Use

Tags

Expression

Bacterial

Mutation

a six amino acid_TRLTEG_insertion in the L1 region

Promoter

Available since

Aug. 12, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge