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Plasmid#222992PurposeMammalian expression of iNme2Cas9 construct with EGFP-targeting sgRNADepositorInsertHuEx-1xNLS-iNme2Cas9-npNLS_EGFP-T1
UseCRISPRTagsPuroExpressionMammalianMutationPromoterpCAGAvailable sinceJuly 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAG-1BPNLS-Cas9-1BPNLS
Plasmid#87108PurposeBPNLS-Cas9-BPNLS expression plasmidDepositorInsert1BPNLS-Cas9-1BPNLS
UseCRISPRTags3xFLAGExpressionMammalianMutationPromoterCAGAvailable sinceMarch 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
AAV_NLS-SaCas9-NLS-VPR
Plasmid#68496PurposeAAV vector containing SaCas9 fused to VPRDepositorInsertSaCas9-VPR
UseAAV and CRISPRTagsVPRExpressionMammalianMutationPromoterCMVAvailable sinceSept. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
PB-CMV-NLS-dCas9-NLS
Plasmid#99908PurposeFor the expression of NLS-dCas9-NLSDepositorInsertNLS-dCas9-NLS
UseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSimpleII-NLS-NmCas9-HA-NLS(s)
Plasmid#47867PurposeThis plasmid encode NmCas9 with two NLS and a HA tagDepositorInsertNmCas9
UseCRISPRTagsHA and NLSExpressionMammalianMutationPromoterEF1aAvailable sinceSept. 25, 2013AvailabilityAcademic Institutions and Nonprofits only -
pUB-nHyPer2 (NLS:HyPer2:NLS)
Plasmid#84730PurposeExpresses HyPer2 in plant cells-biosensor for Hydrogen peroxide targeted to NucleiDepositorInsertHyPer2 targeted to nucleus
UseTagsNLS (nuclear localisation signal and NLS (nuclear…ExpressionPlantMutationPromoterubiqutin- 10 gene promoter (pUBQ10) of ArabidopsisAvailable sinceJuly 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCSD_2xNLS-SpCas9MT3-NLS-FKBP_SL
Plasmid#107305PurposeExpresses R1335K mutant SpCas9 fused to FKBP with extra NLS in mammalian cellsDepositorInsertSpCas9
UseCRISPRTags2x NLS and FKBPExpressionMammalianMutationR1335K and fused to FKBPPromoterCMV IE94Available sinceNov. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
His-CL7_MBP_1xNLS_iGeoCas9(C1)_2xNLS(star)
Plasmid#222990PurposeBacterial expression of iGeoCas9(C1) constructDepositorInsertHis-CL7_MBP_1xNLS_iGeoCas9(C1)_2xNLS(star)
UseCRISPRTagsCL7, His, and MBPExpressionBacterialMutationPromotertacAvailable sinceJuly 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSBbi-NLSmCherryNLS Clover-HDHB
Plasmid#190223PurposeFluorescent cell cycle reporterDepositorInsertsUseTransposonTagsClover fluorescent proteinExpressionMammalianMutationAmino Acids 994-1087PromoterEF1a and RPBSAAvailable sinceSept. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSLQ9757: CMV-BPNLS_TadA-linkerA-TadA*(8e)-Linker B-SV40 NLS-dCasMINI-V4-linkerC-c-Myc_NLS-3x FLAG-polyA
Plasmid#176270PurposeExpression of TadA-TadA* (8e)-dCasMINI-V4 in mammalian cellsDepositorInsertTadA-TadA*(8e)-dCasMINI-V4
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
NLS-dCas9-HA-NLS-GFP-VP16
Plasmid#183923PurposeVP16 activation domain coupled to catalytically inactive dCas9 for localization; labeled with GFPDepositorInsertdCas9-GFP-VP16
UseCRISPRTagsNLSExpressionMammalianMutationnonePromoterCMVAvailable sinceJune 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-NLS-dPspCas13b-3xmRuby3-NLS
Plasmid#132405Purposeoverexpression in human cellsDepositorInsertdPspCas13b
UseLentiviralTagsExpressionMammalianMutationH133A; H1059APromoterAvailable sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-NLS-dPguCas13b-3xmRuby3-NLS
Plasmid#132406Purposeoverexpression in human cellsDepositorInsertdPguCas13b
UseLentiviralTagsExpressionMammalianMutationH151A; H1121APromoterAvailable sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHRm-NLS-dCas9-GFP11x7-NLS
Plasmid#70224PurposeExpresses NLS-dCas9-GFP11x7-NLS in mammalian cellsDepositorInsertNLS-dCas9-GFP11x7-NLS
UseTagsGFP11x7ExpressionMammalianMutationPromoterAvailable sinceMarch 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-TO-nls-st1dCas9-3nls-3XGFP
Plasmid#64111PurposeExpression of St1 dCas9-3XGFP in mammalian cellsDepositorInsertSt1 dCas9
UseCRISPR and LentiviralTags3XsfGFPExpressionMammalianMutationPromoterCMV-TOAvailable sinceApril 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
NLS-dCas9-HA-NLS--GFP-p300
Plasmid#183925Purposep300 HAT core domain coupled to catalytically dead dCas9 for localization; labeled with GFPDepositorInsertdCas9-GFP-p300
UseCRISPRTagsNLSExpressionMammalianMutationnonePromoterCMVAvailable sinceJuly 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRN3P-NLS-EGFP-deadBtgh-3NLS
Plasmid#194470PurposeEncodes for catalytically inactive bacterial O-GlcNAcase BtGH84 fused to enhanced GFP and NLSs for effective nuclear localization, in backbone for T3 In Vitro Transcription.DepositorInsertBtGH84
UseFor in vitro transcription from t3 promoter after…TagsSV40 Nuclear Localization Signal, SV40 Nuclear Lo…ExpressionMutationAspartic Acid 242 (in Dennis et al.) changed to A…PromoterAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAG-1BPNLS-Cas9-1BPNLS-2AGFP
Plasmid#87109PurposeBPNLS-Cas9-BPNLS-2AGFP expression plasmidDepositorInsert1BPNLS-Cas9-1BPNLS-2A-EGFP
UseCRISPRTags3xFLAG (on Cas9)ExpressionMammalianMutationPromoterCAGAvailable sinceMarch 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-TO-nls-st1dCas9-3nls-3XTagBFP2
Plasmid#64512PurposeExpression of St1 dCas9-3XTagBFP2 in mammalian cellsDepositorInsertSt1 dCas9
UseCRISPR and LentiviralTags3XmTagBFP2ExpressionMammalianMutationPromoterCMV-TOAvailable sinceMay 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pmax-3xFLAG_3xNLS_dPspCas13b(AAAA)_2xNLS-Clover
Plasmid#196829PurposeExpress dpdpCas13b with AAAA mutation and CloverDepositorInsertdpspCas13b(AAAA)
UseTags3XFLAG and NESExpressionMammalianMutationK367A, D369A and K370APromoterAvailable sinceMarch 29, 2024AvailabilityAcademic Institutions and Nonprofits only