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Plasmid#174404PurposeBacterial expression of sarA-gp130 chimera under native promoter.DepositorInsertsarA-IL6ST chimera
UseTagsExpressionBacterialMutationPromoterEndogenousAvailable sinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pWSK129-stm2585_S130A
Plasmid#174403PurposeBacterial expression of sarA with its GSK3-binding SxxxSP motif mutated to SxxxAP.DepositorInsertsarA
UseTagsExpressionBacterialMutationsarA with S130A mutationPromoterAvailable sinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pWSK129-stm2585_Y167F
Plasmid#174402PurposeBacterial expression of sarA with its STAT3-binding YxxQ motif mutated to FxxQDepositorInsertsarA
UseTagsExpressionBacterialMutationsarA with Y167F mutationPromoterEndogenousAvailable sinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-Flag-stm2585_L139*
Plasmid#174400PurposeS. Typhimurium gene stm2585 (sarA/steE) truncated after 139th amino acid, which removes the stretch homologous to gp130DepositorInsertsarA
UseTagsEGFP and FlagExpressionMammalianMutationL139* (Truncation after 139th amino acid in sarA)PromoterAvailable sinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-Flag-stm2585_S130A
Plasmid#174399PurposeNative S. Typhimurium gene stm2585 (sarA/steE) with N-terminal GFP and Flag tags and its GSK3-binding SxxxSP motif mutated to SxxxAPDepositorInsertsarA
UseTagsEGFP and FlagExpressionMammalianMutationS130APromoterAvailable sinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-Flag-stm2585_Y167F
Plasmid#174398PurposeNative S. Typhimurium gene stm2585 (sarA/steE) with N-terminal GFP and Flag tags and its STAT3-binding YxxQ motif mutated to FxxQDepositorInsertsarA
UseTagsEGFP and FlagExpressionMammalianMutationY167FPromoterAvailable sinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Flag-gp130dimer-stm2585
Plasmid#174395PurposeConstitutively active gp130 dimer with codon-optimized stm2585 insert (replacing amino acids 140-179)DepositorInsertIL6ST cytosolic domain and sarA chimera
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Flag-stm2585-gp130
Plasmid#174393PurposeChimera of codon-optimized stm2585 (sarA/steE) and human gp130 sequence.DepositorInsertsarA-IL6ST chimera
UseTagsFlagExpressionMammalianMutation140-177 of sarA replaced with homologous IL6ST se…PromoterAvailable sinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Flag-stm2585_Y167F
Plasmid#174392PurposeS. Typhimurium gene stm2585 (sarA/steE) codon-optimized for mammalian expression with STAT3-binding YxxQ motif mutated to FxxQDepositorInsertstm2585
UseTagsFlagExpressionMammalianMutationTyr 167 changed to PhePromoterAvailable sinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Flag-stm2585
Plasmid#174391PurposeS. Typhimurium gene stm2585 (sarA/steE) codon-optimized for mammalian expression with N-terminal Flag tagDepositorInsertstm2585
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pENTR-L1R5-floxHygpA
Plasmid#139559Purposefloxed Hygromycin resistant gene with stop codon franking to pA sequeneces between attL1-attR5 on Gateway entry vectorDepositorInsertHygromycin resistant gene
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
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