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pGC381

Plasmid

#19663

Depositor

Jane Hubbard

Article

Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

Insert

<*-CFP::let-858(3’)

Use

Flp/frt

Tags

Expression

Worm

Mutation

Promoter

Available since

Feb. 23, 2009

Availability

Academic Institutions and Nonprofits only

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pHW394 (15xUAS::GFP::let-858 3'UTR)

Plasmid

#85584

Purpose

cGAL effector (15xUAS::GFP::let-858 3'UTR) construct for C. elegans

Depositor

Paul Sternberg

Article

Wang et al Nat Methods. 2016 Dec 19. doi: 10.1038/n

Insert

15xUAS-delta pes-10-GFP

Use

Tags

Expression

Worm

Mutation

GFP(S65C) with three introns for expression in C.…

Promoter

15xUAS-delta pes-10-GFP

Available since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pCFJ601 - Peft-3 Mos1 transposase

Plasmid

#34874

Purpose

Mos1 transposase expression vector for use in creating targeted transgene insertions and targeted deletions in C. elegans

Depositor

Erik Jorgensen

Article

Frokjaer-Jensen et al Nat Methods. 2012 Jan 30;9(2):117-8. doi

Insert

Mos1 transpoase

Use

Worm targeting

Tags

Expression

Mutation

Promoter

eef-1A.1 (eft-3)

Available since

Feb. 1, 2012

Availability

Academic Institutions and Nonprofits only

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pCFJ356 - MCS(cxTi10816, IV)

Plasmid

#34871

Depositor

Erik Jorgensen

Article

Frokjaer-Jensen et al Nat Methods. 2012 Jan 30;9(2):117-8. doi

Insert

cxTi10816 targeting

Use

Worm targeting

Tags

Expression

Mutation

Promoter

Available since

Feb. 1, 2012

Availability

Academic Institutions and Nonprofits only

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pMDJ39 - Peft-3 Cre (PATCs)

Plasmid

#191381

Purpose

High efficiency CRE recombinase expression in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Insert

Peft-3 Cre tbb-2 UTR

Use

Tags

Expression

Worm

Mutation

Promoter

Peft-3

Available since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pMDJ231 - Phsp Cas9

Plasmid

#191382

Purpose

Heat shock inducible Cas9 expression in C. elegans.

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Insert

Phsp-16.41 Cas9 gpd-2 tagRFP-t

Use

CRISPR

Tags

Expression

Worm

Mutation

Promoter

Phsp-16.41

Available since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pJJR82

Plasmid

#75027

Purpose

EGFP^SEC^3xFlag vector with ccdB markers for cloning homology arms

Depositor

Mike Boxem

Article

Ramalho et al Development. 2020 Jul 22;147(14):dev1880

Insert

EGFP^SEC^3xFlag

Use

CRISPR

Tags

3xFLAG and C. elegans codon optimized EGFP

Expression

Worm

Mutation

Promoter

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pET_SZ1-RGG-GFP-RGG

Plasmid

#124937

Purpose

Expression of SZ1-RGG-GFP-RGG

Depositor

Benjamin Schuster, Matthew Good, Daniel Hammer

Article

Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

Insert

SYNZIP1-RGG-GFP-RGG (using LAF-1 RGG domain) (laf-1 Nematode)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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pSEM319 - array co-injection fragment mosTI unc-119

Plasmid

#159823

Purpose

Single copy or array insertion by MosTI using split unc-119 selection

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Insert

array co-injection fragment mosTI unc-119

Use

Tags

Expression

Worm

Mutation

Not applicable

Promoter

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pJJR83

Plasmid

#75028

Purpose

mCherry^SEC^3xFlag vector with ccdB markers for cloning homology arms

Depositor

Mike Boxem

Article

Ramalho et al Development. 2020 Jul 22;147(14):dev1880

Insert

mCherry^SEC^3xFlag

Use

CRISPR

Tags

3xFLAG and C. elegans codon optimized mCherry

Expression

Worm

Mutation

Promoter

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pMZ9

Plasmid

#198786

Purpose

Drives specific expression in the AWC (ON) neurons

Depositor

Paul Sternberg

Article

Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

Insert

Psrt-28-GAL4-SK(DBD)-VP64

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

May 3, 2023

Availability

Academic Institutions and Nonprofits only

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pCC14

Plasmid

#198781

Purpose

Drives specific expression in the ASJ neurons

Depositor

Paul Sternberg

Article

Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

Insert

Ptrx-1-GAL4-SK(DBD)-VP64

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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pMZ10

Plasmid

#198787

Purpose

Drives specific expression in the AWC (OFF) neurons

Depositor

Paul Sternberg

Article

Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

Insert

Psrt-47-GAL4-SK(DBD)-VP64

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

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pWP005

Plasmid

#198785

Purpose

Drives specific expression in the AWB neurons

Depositor

Paul Sternberg

Article

Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

Insert

Pstr-1-GAL4-SK(DBD)-VP64

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

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pJL065

Plasmid

#198784

Purpose

Drives specific expression in the AWA neurons

Depositor

Paul Sternberg

Article

Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

Insert

Podr-10-GAL4-SK(DBD)-VP64

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

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pWP061

Plasmid

#198775

Purpose

Drives specific expression in the ASE neurons

Depositor

Paul Sternberg

Article

Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

Insert

Pgcy-5-GAL4-SK(DBD)-VP64

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

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3xHA-miniTurbo_pAS32

Plasmid

#118222

Purpose

expresses 3xHA-tagged miniTurbo in C. elegans intestine

Depositor

Jessica Feldman

Article

Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

Insert

miniTurbo (BirA mutant)

Use

Tags

3x HA tag

Expression

Worm

Mutation

aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

Promoter

ges-1p

Available since

Jan. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pCZGY3163

Plasmid

#101248

Purpose

GFP11::rps-18, ribosomal protein subunit, bound to C-terminal part of GFP. Shows GFP localized to ribosomes when combined with GFP 1-10.

Depositor

Yishi Jin

Article

Noma et al Elife. 2017 Aug 2;6. pii: e26376. doi: 1

Insert

gfp11::rps-18 (rps-18 Synthetic, Nematode)

Use

Gateway

Tags

GFP11

Expression

Mutation

Promoter

Available since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pET_RGG-RGG

Plasmid

#124941

Purpose

Expresion of RGG-RGG

Depositor

Benjamin Schuster, Matthew Good, Daniel Hammer

Article

Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

Insert

RGG-RGG (using LAF-1 RGG domain) (laf-1 Nematode)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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pEM1 = flp-21::LoxPStopLoxP::npr-1 SL2 GFP

Plasmid

#24033

Depositor

Cori Bargmann

Article

Macosko et al Nature. 2009 Apr 30. 458(7242):1171-5.

Insert

Npr1 (npr-1 Nematode)

Use

Cre/Lox

Tags

GFP

Expression

Worm

Mutation

Promoter

Available since

Feb. 24, 2010

Availability

Academic Institutions and Nonprofits only

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