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Plasmid#103034Purpose[Edit] Plasmid for IvT reaction with T3. Encodes LINE-1 specific TALE sequence followed by KRAB domainDepositorInsertTAL-L1-1.3-KRAB
UseT3 in vitro transcription for expression in mamma…TagsHA and KRABExpressionMutationPromoterAvailable sinceNov. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pR540
Plasmid#207885Purposetemplate for 2 gRNA amplificationDepositorInsertscaffold-pU6
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCS021
Plasmid#207917PurposeLevel 0 for gRNA in E2 module (5' CCAT-3' ACTA)DepositorInsertpU6-AarI-scaffold-term
UseCRISPRTagsExpressionMutationPromoterAvailable sinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKE013
Plasmid#207916PurposeLevel 0 for gRNA in E1 module (5' CTGC-3' CCAT)DepositorInsertpU3-AarI-scaffold-term
UseCRISPRTagsExpressionMutationPromoterAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
px458_2A_GFP_sgRNA_TIA1
Plasmid#106097PurposeCRISPR knockout. Expresses Cas9, EGFP, and sgRNA targeting TIA1DepositorInsertgRNA TIA1
UseCRISPRTagsExpressionMutationPromoterAvailable sinceAug. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSTARR-seq_fly-RpS12
Plasmid#71504PurposeflySTARR-seq screening vector with RpS12 core promoterDepositorTypeEmpty backboneUseFlystarr-seq screening vectorTagssgGFP (SuperGlo, Qbiogene, Inc)ExpressionInsectMutationPromoterAvailable sinceDec. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGAL4-DBD-MCS-long
Plasmid#145246PurposeBackbone vector expressing GAL4 DNA-binding domain followed by MCSDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMSCVpuro-T7-hAIM2-EGFP
Plasmid#51608PurposeExpress T7 and GFP-tagged hAIM2 in mammalian cellsDepositorInsertAIM2 (AIM2 Human)
UseRetroviralTagsEGFP and T7ExpressionMammalianMutationPromoterMESVAvailable sinceMarch 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCAG-nDsRedFL-miR124
Plasmid#177806PurposeThe pre-miR-124 sequence has been inserted into the EcoRV of pCAG-nDsRedFL-intron.DepositorInsertmiR-124
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
eGFP-CBP
Plasmid#169211PurposeExpression of eGFP-CBP protein for chitin visualizationDepositorInsertChitinase A1 Chitin Binding Domain
UseTags6xHis, Mxe Gyr Intein, and eGFPExpressionBacterialMutationPromoterT7Available sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FBL6 delta6
Plasmid#19925DepositorInsertFBL6 delta 6 (FBXL6 Human)
UseTagsExpressionMammalianMutationdelta 6PromoterAvailable sinceMay 7, 2009AvailabilityAcademic Institutions and Nonprofits only -
pBx1-VHH template3-3XMyc-Spacer
Plasmid#176210PurposePCR template for generating CeVICA VHH/nanobody libraryDepositorInsertVHH template3-3XMyc-Spacer
UseTagsMyc tagExpressionBacterialMutationPromoterT7Available sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBx1-VHH template2-3XMyc-Spacer
Plasmid#176209PurposePCR template for generating CeVICA VHH/nanobody libraryDepositorInsertVHH template2-3XMyc-Spacer
UseTagsMyc tagExpressionBacterialMutationPromoterT7Available sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV4-Tax(M47)
Plasmid#23286DepositorInsertTax(M47)
UseTagsExpressionMammalianMutationL319R L320SPromoterAvailable sinceApril 29, 2010AvailabilityAcademic Institutions and Nonprofits only -
T7-RelA(S276A)
Plasmid#24153DepositorInsertRelA (RELA Human)
UseTagsT7 gp10ExpressionMammalianMutationS276APromoterAvailable sinceApril 29, 2010AvailabilityAcademic Institutions and Nonprofits only -
pSZT025
Plasmid#140033PurposeKO of PCC 7002 fadD gene/expresses codon-optimized 'tesA gene from E. coli from inducible cLac143 promoterDepositorInsertTruncated thioesterase gene
UseTagsExpressionBacterialMutationPromotercLac143Available sinceApril 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCVL SFFV HA.NLS.I-AniIY2(reo).T2A.TagBFP
Plasmid#31477DepositorInsertSFFV HA NLS Y2 T2A mTagBFP
UseLentiviralTagsHA and NLSExpressionMutationF13Y + S111Y on I-AniI = Y2 "reo" (res…PromoterAvailable sinceAug. 22, 2011AvailabilityAcademic Institutions and Nonprofits only -
pNLRP11major.MYC
Plasmid#131196PurposeMammalian expression of human NLRP11 188SDepositorInsertNLRP11 188S (NLRP11 Human)
UseTagsmycExpressionMammalianMutation188SPromoterpCMVAvailable sinceSept. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJYDN3p
Plasmid#162457PurposePositive control plasmid for pJYDN3n plasmid expression attempts. It contains both eUnaG2 bilirubin dependent fluorescent protein and ALFA-tag for DnbALFA based detection on the cell surface.DepositorInsertAga2p-eUnaG2 (AGA2 )
UseShuttle vector bacteria/yeastTagsALFA tag, HA and myc tags, and eUnaG2 fluorescent…ExpressionMutationPromoterGAL1Available sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET3d::strep-AID
Plasmid#15924DepositorInsertAID (AICDA Human)
UseTagsstrepExpressionBacterialMutationPromoterAvailable sinceOct. 26, 2007AvailabilityAcademic Institutions and Nonprofits only