-
-
pQCXIN-EF1a-mNeonGreen-P2A-Cas9
Plasmid#189807PurposeRetroviral delivery of Cas9DepositorInsertCas9
UseRetroviralTagsExpressionMutationPromoterAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
CAGhP14
Plasmid#84362Purposeexpress FLAG-HA tagged human PRDM14DepositorInsertPRDM14 (PRDM14 Human)
UseTagsFLAG and HAExpressionMammalianMutationsilent mutations at coding silent 1068CtoT, 1629T…PromoterCAGAvailable sinceFeb. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
p3E-U6a-U6c
Plasmid#107599PurposeAn entry vector with U6a and U6c promoter driving guide RNAs expressionDepositorInsertsU6a promoter
U6c promoter
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCLX-M38s/TOP-dGFP
Plasmid#114295PurposeExpression of destabilized GFP under the control of the M38-TOP canonical Wnt signaling responsive promoterDepositorInsertdGFP
UseLentiviralTagsExpressionMutationdestabilized GFPPromoterAvailable sinceSept. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
3922-48
Plasmid#176497PurposepTRE-EGFP-T2A-TFEB(wt)-polyADepositorInsertEGFP-T2A-TFEB(wild-type; full-length; human)
UseTagsExpressionMammalianMutationPromoterTREAvailable sinceNov. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti_C_Myc_DDK_IRES_Puro_PRKRA_delta23
Plasmid#106117PurposeLentiviral expression of PRKRA delta23DepositorInsertPRKRA (PRKRA Human)
UseLentiviralTagsDDK (FLAG) and mycExpressionMutationAA 1-127PromoterAvailable sinceFeb. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pIK127
Plasmid#65631PurposeExpresses gfp::h2b in C. elegans cells from the ubiquitous eef-1A.1 (eft-3) promoter.DepositorInsertPeft-3::gfp::h2b::tbb-2 3'UTR
UseTagsExpressionWormMutationPromoterAvailable sinceJune 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
TAL-L1-1.3-Ctrl
Plasmid#102355PurposePlasmid for IvT reaction with T3. Encodes LINE-1 specific TALE sequence followed by multi cloning siteDepositorInsertTAL-L1-1.3
UseT3 in vitro transcription for expression in mamma…TagsFLAG and HAExpressionMutationPromoterAvailable sinceNov. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
EGFP-Tubulin.K40Q
Plasmid#32912DepositorInsertTubulin (TUBA1B Human)
UseTagsEGFPExpressionMammalianMutationK40QPromoterCMVAvailable sinceNov. 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
SARS-CoV-2_N_deltaNTD
Plasmid#157869Purposeexpress MBP tagged SARS-CoV-2 Nucleocapsid protein without the N terminal domainDepositorInsertN (N SARS-CoV-2)
UseTagsMBPExpressionBacterialMutationdeltaNTDPromoterAvailable sinceNov. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGAN147
Plasmid#129202Purposeyeast transcriptional reporter of aggregating proteins (yTRAP) to detect protein aggregation, Gateway backboneDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionYeastMutationPromoterSUP35Available sinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
Nephrin
Plasmid#186134PurposeFor expression of recombinant nephrin in bacteria cellsDepositorInsertNPHS1 (NPHS1 Human)
UseTagsHis6 and MBPExpressionBacterialMutationAmino acid residues 1174-1223, with mutations: Y1…PromoterLacAvailable sinceJune 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV2-hNCOA6:V5
Plasmid#63585PurposeA subunit of the Trithorax-related (Trr) methyltransferase complex.DepositorInsertNCOA6 (NCOA6 Human)
UseTags6xHis and V5ExpressionMammalianMutationPromoterCMVAvailable sinceNov. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
Phat2-hs-cPla2-C2-EGFP-GH
Plasmid#187088PurposeProtein ExpressionDepositorInsertENSG00000116711 (PLA2G4A Human)
UseTagsEGFPExpressionBacterialMutationPromoterAvailable sinceAug. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pErbB2-ECFP
Plasmid#66945PurposeMammalian expression of rat ErbB2 tagged with ECFPDepositorInsertErbB2 (Erbb2 Rat)
UseTagsECFPExpressionMammalianMutationPromoterCMVAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCfB9337 (pgRNA_IV-1_NatMX)
Plasmid#161590PurposeEasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site IV-1DepositorInsertguiding RNA
UseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceAug. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-HA-mIFITM1
Plasmid#58415PurposeExpresses murine IFITM1 with an N-terminal HA tag in mammalian cells.DepositorInsertIFITM1 (Ifitm1 Mouse)
UseTagsHAExpressionMammalianMutationPromoterCMV IEAvailable sinceSept. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pT-GEM(1)
Plasmid#62893PurposeTrojan-Gal4 Expression Module in Phase 1. Insert T2A-Gal4 to genomic loci using the Crispr/Cas technology. Can be converted by cassette exchange into other Trojan exonsDepositorTypeEmpty backboneUseTagsExpressionMutationPromoterAvailable sinceMay 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
TAL-L1-1.3-VP64
Plasmid#103033PurposePlasmid for IvT reaction with T3. Encodes LINE-1 specific TALE sequence followed by VP64 activatorDepositorInsertTAL-L1-1.3-VP64
UseT3 in vitro transcription for expression in mamma…TagsHA and VP64ExpressionMutationPromoterAvailable sinceNov. 30, 2017AvailabilityAcademic Institutions and Nonprofits only
