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Plasmid#210484PurposepDEST vector to tag gene of interest with mEGFP on the C-terminus to perform localization assays in S. cerevisiae.DepositorTypeEmpty backboneUseSynthetic Biology; Destination vector for gateway…TagsmEGFPExpressionYeastMutationPromoterADH1Available sinceDec. 22, 2023AvailabilityAcademic Institutions and Nonprofits only
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pMuLE (R4-R3) PRB
Plasmid#206164PurposepMuLE R4-R3 entry plasmid containing human full length PRB gene, for 3-component Gateway assemblyDepositorInsertProgesteron receptor B (PGR Human)
UseGateway cloning vectorTagsExpressionMutationPromoter-Available sinceNov. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
MLKS2delARMec
Plasmid#130860PurposeExpression of plant nuclear envelope LINC protein fused to fluorescent protein and affinity tags for cell biologyDepositorInsertMLKS2
UseGateway entry vectorTagseGFP-FLAG-HAExpressionMutationDeletion of armadillo domainPromoterAvailable sinceSept. 28, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
MLKS2del4ec
Plasmid#130857PurposeExpression of plant nuclear envelope LINC protein fused to fluorescent protein and affinity tags for cell biologyDepositorInsertMLKS2
UseGateway entry vectorTagseGFP-FLAG-HAExpressionMutationDeletion of terminal four amino acidsPromoterAvailable sinceSept. 28, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
MLKS2delKASHec
Plasmid#130858PurposeExpression of plant nuclear envelope LINC protein fused to fluorescent protein and affinity tags for cell biologyDepositorInsertMLKS2
UseGateway entry vectorTagseGFP-FLAG-HAExpressionMutationeGFP-FLAG-HAPromoterAvailable sinceSept. 28, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pEN_TT 3xFLAG-BCAS2
Plasmid#192294PurposeGateway entry vector for an inducible 3xFLAG-BCAS2DepositorInsertBCAS2 (BCAS2 Human)
UseGateway entry vectorTags3xFLAGExpressionMutationPromoterAvailable sinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
CPL2g/pDONR221
Plasmid#172093PurposeGenomic fragment containing the upstream region and coding region of CER11/CPL2 cloned into pDONR221DepositorInsertCPL2 (CPL2 Mustard Weed)
UseGateway entry vectorTagsExpressionMutationPromoterCPL2Available sinceOct. 20, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDONR207-MUM2sp-PelA
Plasmid#170722PurposeGateway (Invitrogen) entry clone (pDONR207) containing the MUM2 (At5g63800) signal peptide (84bp) fused in-frame to the coding sequence of the HG degrading enzyme PelA (AN2331.2)DepositorInsertPectin lyase A
UseGateway donor vector / entry cloneTagsExpressionMutationPromoterAvailable sinceOct. 20, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDONR207-MUM2sp-RglB
Plasmid#170721PurposeGateway (Invitrogen) entry clone (pDONR207) containing the MUM2 (At5g63800) signal peptide (84bp) fused in-frame to the coding sequence of the RG-I degrading enzyme RglB (AN6395.2)DepositorInsertRhamnogalacturonan lyase B
UseGateway donor vector / entry cloneTagsExpressionMutationPromoterAvailable sinceOct. 20, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDONR207-MUM2sp-RglA
Plasmid#170720PurposeGateway (Invitrogen) entry clone (pDONR207) containing the MUM2 (At5g63800) signal peptide (84bp) fused in-frame to the coding sequence of the RG-I degrading enzyme RglA (AN7135.2)DepositorInsertRhamnogalacturonan lyase A
UseGateway donor vector / entry cloneTagsExpressionMutationPromoterAvailable sinceOct. 20, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pENTR-D-kcnj15
Plasmid#173145PurposeThis gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.DepositorInsertkcnj15 (kcnj15 Zebrafish)
UsePcr cloning vectorTagsExpressionMutationPromoterNo promoter at 5 end; T7 promoter at 3 end.Available sinceSept. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pENTR-D-kcnj1a.1
Plasmid#173125PurposeThis gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.DepositorInsertkcnj1a (kcnj1a.1 Zebrafish)
UsePcr cloning vectorTagsExpressionMutationPromoterNo promoter at 5 end; T7 promoter at 3 end.Available sinceSept. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
p3E-APEX2 (GC rich)
Plasmid#115550PurposeMultisite gateway vector for 3' tagging with GC-rich APEX2. Used to make 115563. Parton lab clone HHSDepositorInsertAPEX2 (GC rich)
UseMultisite gateway entry cloneTagsExpressionMutationPromoterAvailable sinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCFJ1972 - [1-2] ENTR - Flour - TagBFP2(NLS(2), no_atg, no_stop)
Plasmid#159844PurposeThree-fragment Gateway compatible flourophore for expression in C. elegansDepositorInsert[1-2] ENTR - Flour - TagBFP2(NLS(2), no_atg, no_stop)
UseTagsExpressionWormMutationNot applicablePromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pME-TCAP
Plasmid#109549PurposeMultisite gateway vector for 5' tagging with telethoninDepositorInserttelethonin (tcap Zebrafish)
UseZebrafish plasmidsTagsExpressionMutationPlease see Depositor CommentsPromoterAvailable sinceMarch 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
p3E-rab26-DN
Plasmid#109745PurposeMultisite gateway vector for 3' tagging with dominant negative rab26DepositorInsertrab26 (rab26 Zebrafish)
UseZebrafish plasmidsTagsExpressionMutationdominant negativePromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
p3E-rab26-CA
Plasmid#109746PurposeMultisite gateway vector for 3' tagging with constitutively active rab26DepositorInsertrab26 (rab26 Zebrafish)
UseZebrafish plasmidsTagsExpressionMutationconstitutively activePromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
p3E-rab38a-CA
Plasmid#109748PurposeMultisite gateway vector for 3' tagging with constitutively active rab38aDepositorInsertrab38a (rab38a Zebrafish)
UseZebrafish plasmidsTagsExpressionMutationconstitutively activePromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
p3E-rab6a-CA
Plasmid#109733PurposeMultisite gateway vector for 3' tagging with constitutively active rab6aDepositorInsertrab6a (rab6a Zebrafish)
UseZebrafish plasmidsTagsExpressionMutationconstitutively activePromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
p3E-rab23-DN
Plasmid#109734PurposeMultisite gateway vector for 3' tagging with dominant negative rab23DepositorInsertrab23 (rab23 Zebrafish)
UseZebrafish plasmidsTagsExpressionMutationdominant negativePromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only