pMuLE (R4-R3) PRB
(Plasmid
#206164)
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PurposepMuLE R4-R3 entry plasmid containing human full length PRB gene, for 3-component Gateway assembly
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 206164 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepMuLE (R4-R3) ENTR MCS
- Backbone size w/o insert (bp) 3729
- Total vector size (bp) 6531
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Vector typeGateway cloning vector
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameProgesteron receptor B
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Alt namePRB, PGR
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SpeciesH. sapiens (human)
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Insert Size (bp)2802
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GenBank IDNM_000926.4
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Entrez GenePGR (a.k.a. NR3C3, PR)
- Promoter -
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Acc65I (destroyed during cloning)
- 3′ cloning site EcoRI (destroyed during cloning)
- 5′ sequencing primer M13 forward
- 3′ sequencing primer M13 reverse (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byPRB gene is derived from Addgene plasmid #89130
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMuLE (R4-R3) PRB was a gift from Renee van Amerongen (Addgene plasmid # 206164 ; http://n2t.net/addgene:206164 ; RRID:Addgene_206164) -
For your References section:
A molecular toolbox to study progesterone receptor signaling. Aarts MT, Wagner M, van der Wal T, van Boxtel AL, van Amerongen R. J Mammary Gland Biol Neoplasia. 2023 Nov 29;28(1):24. doi: 10.1007/s10911-023-09550-0. 10.1007/s10911-023-09550-0 PubMed 38019315