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Addgene

pMuLE (R4-R3) PRB
(Plasmid #206164)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 206164 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pMuLE (R4-R3) ENTR MCS
  • Backbone size w/o insert (bp) 3729
  • Total vector size (bp) 6531
  • Vector type
    Gateway cloning vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Progesteron receptor B
  • Alt name
    PRB, PGR
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2802
  • GenBank ID
    NM_000926.4
  • Entrez Gene
    PGR (a.k.a. NR3C3, PR)
  • Promoter -

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Acc65I (destroyed during cloning)
  • 3′ cloning site EcoRI (destroyed during cloning)
  • 5′ sequencing primer M13 forward
  • 3′ sequencing primer M13 reverse
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    PRB gene is derived from Addgene plasmid #89130

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMuLE (R4-R3) PRB was a gift from Renee van Amerongen (Addgene plasmid # 206164 ; http://n2t.net/addgene:206164 ; RRID:Addgene_206164)
  • For your References section:

    A molecular toolbox to study progesterone receptor signaling. Aarts MT, Wagner M, van der Wal T, van Boxtel AL, van Amerongen R. J Mammary Gland Biol Neoplasia. 2023 Nov 29;28(1):24. doi: 10.1007/s10911-023-09550-0. 10.1007/s10911-023-09550-0 PubMed 38019315