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Plasmid#184909PurposeExpresses the Streptococcus pyogenes Cas9 proteinDepositorInsertCas9
UseCRISPRTagsExpressionBacterial and YeastMutationPromoterAvailable sinceJan. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
HM3C NaVAe1_p pET24b
Plasmid#127854PurposeBacterial expression vector for NaVAe1 bacterial sodium channel residues 143-288. It expresses HIStag-MBP-3C-channel protein.DepositorInsertNaVAe1p
UseTags3C Cleavage site, His, and MBPExpressionBacterialMutationPromoterAvailable sinceMay 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLV U6-sgRNA UbC-tdTomato
Plasmid#106949PurposesgRNA cloning cassette using BsmBI/Esp3I enzymes with tdTomato markerDepositorInsertsgRNA BsmBI/Esp3I cloning site
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceDec. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
plv-AcGFP-SOD1 (G93A)
Plasmid#27142DepositorInsertSOD1 (SOD1 Human)
UseLentiviralTagsAcGFPExpressionMutationG93APromoterAvailable sinceMarch 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5FRT PUR mGSK3AWT
Plasmid#35159DepositorInsertGSK-3α (Gsk3a Mouse)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 18, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA3.1-StrepHA-SUMO2
Plasmid#66867PurposeMammalian expression of SUMO2 with a strep and an HA tagDepositorInsertSUMO2 (SUMO2 Human)
UseTagsHA and StrepExpressionMammalianMutationPromoterCMVAvailable sinceJune 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pT3TS-spCas9-ABE-Umax-rest1
Plasmid#222144PurposeAdenine base editor with modified TadA variant for restricted A-to-G base editing in zebrafishDepositorInsertModified TadA-nSpCas9
UseCRISPR; Zebrafish expressionTagsBipartite NLSExpressionMutationPromoterT3Available sinceJuly 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
plv-AcGFP-SOD1 (wt)
Plasmid#27138DepositorInsertSOD1 (SOD1 Human)
UseLentiviralTagsAcGFPExpressionMutationPromoterAvailable sinceMarch 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
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CMV::TVA/tRFP
Plasmid#164486PurposeDual CMV promoter plasmid to express TVA and TurboRFPDepositorInsertsUseTagsExpressionMammalianMutationPromotersCMV and sCMV IE94Available sinceJan. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSBbi_RP_3XNLS-MicroID2-FLAG
Plasmid#188523PurposeEncodes the biotin proximity labeling enzyme MicroID2 with a 3X nuclear localization sequence (NLS) for expression and stable integration into mammalian cellsDepositorInsert3XNLS-MicroID2
UseTransposon mediated gene integrationTagsFLAGExpressionMammalianMutationPromoterEF1a/RPBSAAvailable sinceSept. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCDNA6-IgK-hFRa(23-234)C11S-th-Fc
Plasmid#177841PurposeMammalian Expression of Folate ReceptorDepositorInsertFolate receptor alpha (FOLR1 Human)
UseTagsExpressionMammalianMutationall 11 C mutated to SPromoterAvailable sinceDec. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pGDP1 NDM-1
Plasmid#112883PurposeThis plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.DepositorInsertNDM-1 (blaNDM-1 Klebsiella pneumoniae)
UseTags6xHisExpressionBacterialMutationPromoterPblaAvailable sinceDec. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
POT2-RFP
Plasmid#65318Purposereceiving vector of standard transcription units(TUs) in YeastFab work, useful for further assemblyDepositorInsertRFP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDR-GFPuniv
Plasmid#46085PurposeIn vivo recombination assay fluorescent reporterDepositorInsertDR-GFPuniv reporter
UseRecombination reporter plasmidTagsExpressionMammalianMutationE5G and K163R mutations in EGFP relative to wild-…PromoterAvailable sinceMay 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
LentiCRISPR v2 hBAX
Plasmid#129580PurposeCRISPR deletion of human BAXDepositorInsertgRNA vs human BAX (BAX Human)
UseLentiviralTagsExpressionMammalianMutationNonePromoterAvailable sinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
NSP3-FR-2Strep
Plasmid#210315PurposeExpresses SARS-CoV-2 NSP3 in mammalian cellsDepositorInsertNSP3 (ORF1ab SARS-CoV-2)
UseTags2StrepExpressionMammalianMutationPromoterAvailable sinceDec. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEX-CMV-SP-STIM2(15-746)
Plasmid#18868DepositorInsertSTIM2 (STIM2 Human)
UseTagsExpressionMammalianMutationSignal peptide(SP) 1-10 from STIM1 and residues 1…PromoterAvailable sinceMarch 24, 2009AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Flag-gp130dimer
Plasmid#174394PurposeCytosolic domain of gp130 with C-C linker and Leu zipper for constitutive dimerization and activation (from Stuhlmann-Laeisz et al. 2006).DepositorInsertIL6ST cytosolic domain with leucine zipper & C-C linker
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only