pCDNA6-IgK-hFRa(23-234)C11S-th-Fc
(Plasmid
#177841)
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PurposeMammalian Expression of Folate Receptor
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 177841 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepCDNA6
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameFolate receptor alpha
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SpeciesH. sapiens (human)
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Mutationall 11 C mutated to S
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Entrez GeneFOLR1 (a.k.a. FBP, FOLR, FRalpha, NCFTD)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
IgK = Ig kappa leader, th = thrombin cleavage site, Fc = Igl Fc fragment
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCDNA6-IgK-hFRa(23-234)C11S-th-Fc was a gift from Karsten Melcher (Addgene plasmid # 177841 ; http://n2t.net/addgene:177841 ; RRID:Addgene_177841) -
For your References section:
Structural basis for molecular recognition of folic acid by folate receptors. Chen C, Ke J, Zhou XE, Yi W, Brunzelle JS, Li J, Yong EL, Xu HE, Melcher K. Nature. 2013 Aug 22;500(7463):486-9. doi: 10.1038/nature12327. Epub 2013 Jul 14. 10.1038/nature12327 PubMed 23851396
