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pYPQ269C2

Plasmid

#164716

Purpose

Gateway entry clone (attL1 & attR5) hAID-CBE_V04 for C-T base editing

Depositor

Yiping Qi

Article

Ren et al Plant Biotechnol J. 2021 May 27. doi: 10

Insert

hAID-zCas9(D10A)-UGI-T2A-MS2-UGI

Use

CRISPR; Gateway compatible haid-cbe_v04 entry clo…

Tags

Expression

Plant

Mutation

Promoter

Available since

May 21, 2021

Availability

Academic Institutions and Nonprofits only

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pMX_mTAF3

Plasmid

#44334

Depositor

Marc Timmers

Article

Pijnappel et al Nature. 2013 Mar 28;495(7442):516-9. doi

Insert

TAF3 (Taf3 Mouse)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

LTR

Available since

May 14, 2013

Availability

Academic Institutions and Nonprofits only

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ND4 right TALE–G1333 DddAtox N

Plasmid

#158096

Purpose

expresses the right-side ND4 TALE–DddAtox half in mammalian cells

Depositor

David Liu

Article

Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

Insert

COX8A MTS–3xFLAG–ND4 right TALE–G1333 DddA tox-N–1xUGI

Use

Tags

Expression

Mammalian

Mutation

WT

Promoter

Available since

Sept. 15, 2020

Availability

Academic Institutions and Nonprofits only

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G1333 DddAtox-C–SaKKH-Cas9(D10A)

Plasmid

#157838

Purpose

expresses split DddAtox-Cas9 construct in mammalian cells

Depositor

David Liu

Article

Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

Insert

G1333 DddAtox-C–SaKKH-Cas9(D10A)–UGI–SV40 NLS

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 19, 2020

Availability

Academic Institutions and Nonprofits only

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shNg Lenti FHRC3J1UGW

Plasmid

#92233

Purpose

lentiviral expression of EGFP and Ng shRNA

Depositor

Weifeng Xu

Article

Hwang et al Biol Psychiatry. 2021 Feb 1;89(3):256-26

Insert

shRNA targeting Ng

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

H1

Available since

March 1, 2022

Availability

Academic Institutions and Nonprofits only

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mVenus-IQ6 R6A

Plasmid

#198202

Purpose

FRET acceptor: Mutated IQ6 domain of Moysin Va tagged with N-terminal mVenus (lower binding affinity)

Depositor

Christian Wahl-Schott

Article

Feldmann et al STAR Protoc. 2023 Jul 28;4(3):102459. do

Insert

MYO5A IQ domain (Myo5a Mouse)

Use

Tags

mVenus

Expression

Mammalian

Mutation

R6A

Promoter

Available since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-KSENB-Nup133-mut

Plasmid

#87339

Purpose

To express human Nup133 in mammalian cells. It contains synonymous mutations that are not targeted by shRNA

Depositor

Lei Lu

Article

Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

Insert

NUP133 (NUP133 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

shRNA resistant changes; deletion of Nup133 AA1-7

Promoter

CMV

Available since

May 4, 2017

Availability

Academic Institutions and Nonprofits only

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G1397 DddAtox-N–SaKKH-Cas9(D10A)

Plasmid

#157839

Purpose

expresses split DddAtox-Cas9 construct in mammalian cells

Depositor

David Liu

Article

Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

Insert

G1397 DddAtox-N–SaKKH-Cas9(D10A)–UGI–SV40 NLS

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

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KJ901: pMAGIC (R4-R3) NLS-(SrfI/PmeI)-NLS

Plasmid

#121835

Purpose

pMAGIC R4-R3 entry plasmid, contains 2x NLS scaffold enabling addition of dCas9 mutants into pMAGIC. dCas9 can be inserted via unique SrfI/PmeI restriction sites

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Synthetic Biology; Pmagic gateway entry plasmid

Tags

Expression

Mutation

Promoter

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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MK13_HUMAN_D0

Plasmid

#79693

Purpose

This plasmid encodes the kinase domain of MK13. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

Nicholas Levinson, Markus Seeliger, John Chodera

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

MK13

Use

Tags

His10-TEV

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only