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Plasmid#164714PurposeGateway entry clone (attL1 & attR5) rAPOBEC1-CBE_V04 for C-T base editingDepositorInsertrAPOBEC1-zCas9(D10A)-UGI-T2A-MS2-UGI
UseCRISPR; Gateway compatible rapobec1-cbe_v04 entry…TagsExpressionPlantMutationPromoterAvailable sinceMay 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pZA1
Plasmid#158479PurposeEntry clone for Golden Gate assembly. Golden Gate compatible wild-type A. thaliana ZAR1 with STOP codonDepositorInsertZAR1 (ZAR1 Mustard Weed)
UseGolden gate entry vectorTagsExpressionMutationSTOP codonPromoterAvailable sinceAug. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
PHKG2_HUMAN_D0
Plasmid#79736PurposeThis plasmid encodes the kinase domain of PHKG2. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorInsertPHKG2 (PHKG2 Human)
UseTagsHis10-TEVExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
VRK2_HUMAN_D0
Plasmid#79737PurposeThis plasmid encodes the kinase domain of VRK2. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorInsertVRK2 (VRK2 Human)
UseTagsHis10-TEVExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pYPQ265
Plasmid#164712PurposeGateway entry clone (attL1 & attR5) rAPOBEC1-CBE_V01 for C-T base editingDepositorInsertrAPOBEC1-zCas9(D10A)-UGI
UseCRISPR; Gateway compatible rapobec1-cbe_v01 entry…TagsExpressionPlantMutationPromoterAvailable sinceMay 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
IF311: pMAGIC (R4-R3) NLS-Sa dCas9-NLS-LSD1
Plasmid#121824PurposepMAGIC R4-R3 entry plasmid, contains 2x NLS Sa-dCas9 fused to the LSD1 H3K4me1/2 demethylase for 3 or 4-component MultiSite Gateway Pro assembly.DepositorInsertSa-dCas9/LSD1 (open)
UseSynthetic Biology; Pmagic gateway entry plasmidTagsNLSExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAD/CMV/V5-DEST-Rac1-2G
Plasmid#66112PurposeSecond generation Rac1 FRET biosensor for adenovirus productionDepositorInsertSecond generation Rac1 FRET biosensor
UseAdenoviralTagsHis-MycExpressionMammalianMutationPromoterCMVAvailable sinceOct. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEN_TT 3xFLAG-KPNA1
Plasmid#192304PurposeGateway entry vector for an inducible 3xFLAG-KPNA1DepositorInsertKPNA1 (KPNA1 Human)
UseGateway entry vectorTags3xFLAGExpressionMutationPromoterAvailable sinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMuLE ENTR U6 stuffer sgRNA scaffold R4-R3
Plasmid#62131PurposeMuLE (Multiple Lentiviral Expression) Entry vector containing a U6 promoter and sgRNA scaffold module for gRNA expression. Compatible with MultiSite Gateway cloningDepositorTypeEmpty backboneUseCRISPR; Mule gateway entry vectorTagsExpressionMammalianMutationPromoterAvailable sinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
mEmerald-MyosinIIC-C-18
Plasmid#54194PurposeLocalization: Cytoskeleton, Excitation: 487, Emission: 509DepositorInsertMyosinIIC
UseTagsmEmeraldExpressionMammalianMutationPromoterCMVAvailable sinceJuly 10, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits