-
Plasmid#193450Purposeko TFAP4 guide 1DepositorInsertTfap4
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
KillerOrange /pQE-30
Plasmid#74748PurposeKillerOrange gene for bacterial expressionDepositorInsertKillerOrange
UseTagsHisTagExpressionBacterialMutationKillerRed with substitutions G3C Y66W D113S N145S…PromoterT5Available sinceAug. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
Keratin 8 (pcDNA3)
Plasmid#18063DepositorInsertKeratin 8 (KRT8 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 16, 2008AvailabilityAcademic Institutions and Nonprofits only -
keratin 8 (krt8)-H2B-RFP
Plasmid#105955PurposetransgenesisDepositorInsertkrt8 (KRT8 Human)
UseTagsRFPExpressionBacterialMutationPromoterAvailable sinceFeb. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
Keratin 8 S73A
Plasmid#18056DepositorInsertKeratin 8 (KRT8 Human)
UseTagsExpressionMammalianMutationS73APromoterAvailable sinceMay 16, 2008AvailabilityAcademic Institutions and Nonprofits only -
Keratin 8 S73D
Plasmid#18054DepositorInsertKeratin 8 (KRT8 Human)
UseTagsExpressionMammalianMutationS73DPromoterAvailable sinceMay 16, 2008AvailabilityAcademic Institutions and Nonprofits only -
Keratin 8 S431A
Plasmid#18058DepositorInsertKeratin 8 (KRT8 Human)
UseTagsExpressionMammalianMutationS431APromoterAvailable sinceMay 16, 2008AvailabilityAcademic Institutions and Nonprofits only -
Keratin 8 G61C
Plasmid#18055DepositorInsertKeratin 8 (KRT8 Human)
UseTagsExpressionMammalianMutationG61CPromoterAvailable sinceJune 11, 2008AvailabilityAcademic Institutions and Nonprofits only -
Keratin 8 S23A
Plasmid#18057DepositorInsertKeratin 8 (KRT8 Human)
UseTagsExpressionMammalianMutationS23APromoterAvailable sinceMay 16, 2008AvailabilityAcademic Institutions and Nonprofits only -
pJK528
Plasmid#155388PurposedCas12a oscillator, reverse crRNAs + PA4-mVenus. (Integration of 511; pOSIP)DepositorInsertsdCas12a (F. novicida)
PA4-mVenus
dCas12a oscillator crRNAs
UseCRISPRTagsExpressionBacterialMutationD917A (nuclease-deactivating)PromoterAvailable sinceFeb. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJK434
Plasmid#155378PurposePphlF_A4NT, 1x cascade + [dCas9 + PA4-mVenus]. (Integration of 429; pOSIP)DepositorInsertsdCas9 (bacteria)
PA4-mVenus
PphlF_A4NT in dCas9 sgRNA oscillator v1
UseCRISPRTagsExpressionBacterialMutationD10A H840A (catalytically inactive)PromoterAvailable sinceFeb. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJK422
Plasmid#155373PurposedCas9 oscillator v1 (dCRv1) + [dCas9 + PA4-mVenus]. (Integration of 412; pOSIP)DepositorInsertsdCas9 (bacteria)
PA4-mVenus
dCas9 sgRNA oscillator v1
UseCRISPRTagsExpressionBacterialMutationD10A H840A (catalytically inactive)PromoterAvailable sinceDec. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJK423
Plasmid#155374PurposedCas9 oscillator v1 (dCRv1) + [dCas9 + PA4-mVenus]. (Integration of 413; pOSIP)DepositorInsertsdCas9 (bacteria)
PA4-mVenus
dCas9 sgRNA oscillator v1
UseCRISPRTagsExpressionBacterialMutationD10A H840A (catalytically inactive)PromoterAvailable sinceDec. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pX458_miR-290-295KO-gRNA1
Plasmid#172709PurposeCas9 with 5' gRNA for paired CRISPR KO of miR-290-295 clusterDepositorInsertmiR-290-295
UseCRISPRTagsExpressionMutationPromoterAvailable sinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX458_miR-290-295KO-gRNA2
Plasmid#172710PurposeCas9 with 3' gRNA for paired CRISPR KO of miR-290-295 clusterDepositorInsertmiR-290-295
UseCRISPRTagsExpressionMutationPromoterAvailable sinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
FB012
Plasmid#119709PurposeComplete TU for hygromicin resistance in fungi, domesticated into pUPD2 with AATG/GCTT barcodes. Used for gene KO with dual selection, according to FungalBraid modular DNA assembly for ATMTDepositorInsertPtrpC:hph:Ttub
UseSynthetic Biology; Domestication of dna parts for…TagsExpressionMutationPromoterAvailable sinceMarch 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pX458_miR-290-295KO-gRNA3
Plasmid#172711PurposeCas9 with 5' gRNA for paired CRISPR KO of miR-290-295 clusterDepositorInsertmiR-290-295
UseCRISPRTagsExpressionMutationPromoterAvailable sinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
EED-F97A
Plasmid#131326PurposegRNA to generate F97A cage-mutant EEDDepositorInsertEED-KO-gRNA-2
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceMarch 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
LentiGuidPuro-hTP53_sgRNA-1
Plasmid#88853PurposeCRISPR KO of Trp53DepositorInsertTrp53
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJuly 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLVX-HA-UBE3B-IRES-Puro
Plasmid#107345PurposeExpresses HA tagged UBE3B in mammalian cells.DepositorInsertUBE3B (UBE3B Human)
UseLentiviralTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceApril 19, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits