Showing: 561 - 580 of 663 results
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Plasmid#88849PurposeCRISPR KO of Trp63DepositorInsertTrp63
UseCRISPRTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
Px458-Cas9n-Trp73-Exon4-3sgRNA
Plasmid#88850PurposeCRISPR KO of Trp73DepositorInsertTrp73
UseCRISPRTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
Px458-Cas9n-Trp73-Exon4-4sgRNA
Plasmid#88851PurposeCRISPR KO of Trp73DepositorInsertTrp73
UseCRISPRTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
LentiGuidPuro-hTP53_sgRNA-1
Plasmid#88853PurposeCRISPR KO of Trp53DepositorInsertTrp53
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
LentiGuidPuro-hTP63_sgRNA-1
Plasmid#88854PurposeCRISPR KO of Trp63DepositorInsertTrp63
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
LentiGuidPuro-hTP73_sgRNA-1
Plasmid#88855PurposeCRISPR KO of Trp73DepositorInsertTrp73
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pCAG-eSpCas9-2A-GFP-sgRNA-DNA-PKcs
Plasmid#220493PurposeTo generate DNA-PKcs KO in human cells. Co-expresses eSpCas9(1.1), GFP and a guide RNA against DNA-PKcs exon 1.DepositorInsertsgRNA targeting DNA-PKcs (PRKDC) exon 1 (PRKDC Human)
UseCRISPRTagsExpressionMutationPromoterU6AvailabilityAcademic Institutions and Nonprofits only -
pCAG-eSpCas9-2A-GFP-sgRNA-Lig4
Plasmid#220494PurposeTo generate LIG4 KO in human cells. Co-expresses eSpCas9(1.1), GFP and a guide RNA against LIG4 exon 3.DepositorInsertsgRNA targeting LIG4 exon 3 (LIG4 Human)
UseCRISPRTagsExpressionMutationPromoterU6AvailabilityAcademic Institutions and Nonprofits only -
FB013
Plasmid#119710PurposeTU of the HSVtk negative marker for F2dU domesticated into pUPD2 with GGAG/TACT barcodes (reverse orientation). Used for gene KO with dual selec. According to FungalBraid modular DNA assembly for ATMTDepositorInsertNegative selection marker HSVtk (Pgpda:HSVtk:Ttub)
UseSynthetic Biology; Domestication of dna parts for…TagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pCT-CMV-UBE3B-copGFP-Puro
Plasmid#107342PurposeExpresses UBE3B fused to copGFP in mammalian cells.DepositorInsertUbiquitin-protein ligase E3B (UBE3B Human)
UseLentiviralTagscopGFPExpressionMammalianMutationPromoterCMVAvailabilityIndustry, Academic Institutions, and Nonprofits -
pCT-CMV-UBE3BΔHECT-copGFP-Puro
Plasmid#107343PurposeExpresses UBE3B deletion of HECT domain fused to copGFP in mammalian cells.DepositorInsertUBE3B(ΔHECT)-copGFP (UBE3B Human)
UseLentiviralTagscopGFPExpressionMammalianMutationdeletion of HECT domain (757-end)PromoterCMVAvailabilityIndustry, Academic Institutions, and Nonprofits -
pCT-CMV-UBE3B(C1036A)-copGFP-Puro
Plasmid#107344PurposeExpresses UBE3B mutant C1036A fused to copGFP in mammalian cells.DepositorInsertUBE3B(C1036A)copGFP (UBE3B Human)
UseLentiviralTagscopGFPExpressionMammalianMutationmutation C1036APromoterCMVAvailabilityIndustry, Academic Institutions, and Nonprofits -
pLVX-HA-UBE3B(C1036A)-IRES-Puro
Plasmid#107348PurposeExpresses HA tagged UBE3B mutant C1036A in mammalian cells.DepositorInsertUBE3B(C1036A) (UBE3B Human)
UseLentiviralTagsHAExpressionMammalianMutationchanged Cysteine 1036 to AlaninePromoterCMVAvailabilityIndustry, Academic Institutions, and Nonprofits -
pLVX-UBE3BΔIQ-HA-IRES-Puro
Plasmid#107350PurposeExpresses HA tagged UBE3B deletion of IQ domain in mammalian cells.DepositorInsertUBE3BdelIQ (UBE3B Human)
UseLentiviralTagsHAExpressionMammalianMutationPromoterCMVAvailabilityIndustry, Academic Institutions, and Nonprofits -
pMSCV(W-)U6sgRNA5(BbsI)-PGKpuroBFP
Plasmid#102797PurposeRetrovirus for testing CRISPR KO activity (empty) - non-targeting control plasmid contains GFP instead of PuroRDepositorInsertssgRNA
EGFP
UseCRISPR and RetroviralTagsExpressionMammalianMutationH231LPromoterAvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPR v2-sgControl
Plasmid#125836PurposeKO controlDepositorInsertControl
UseLentiviralTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
Donor_YTHDF2_KO_Puro
Plasmid#186670PurposeDonor plasmid to endogenously knock out the YTHDF2 in Hek293 cells. Puromycin and polyA sequence is inserted between two homology arms.DepositorInsertYTHDF2 KO Puromycin (YTHDF2 Human)
UseTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
Donor_YTHDF2_KO_Hygro
Plasmid#186671PurposeDonor plasmid to endogenously knock out the YTHDF2 in Hek293 cells. Hygromycin and polyA sequence is inserted between two homology arms.DepositorInsertYTHDF2 KO Hygromycin (YTHDF2 Human)
UseTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
Donor_YTHDF2_KO_Neo
Plasmid#186672PurposeDonor plasmid to endogenously knock out the ANKRD1 in Hek293 cells. Neomycin and polyA sequence is inserted between two homology arms.DepositorInsertYTHDF2 KO Neomycin (YTHDF2 Human)
UseTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
Cas9_YTHDF2_sgRNA
Plasmid#186673PurposeYTHDF2 sgRNA plasmidDepositorInsertYTHDF2 KO sgRNA Plasmid (YTHDF2 Human)
UseTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only
Showing: 561 - 580 of 663 results