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Plasmid#154193PurposeLentiviral expression plasmid encoding dCas9-VPR and a P2A linked mCherry markerDepositorInsertsp-dCas9-VPR_P2A_mCherry
UseLentiviralTagsExpressionMammalianMutationD10A, H840APromoterEF1asAvailable sinceDec. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFP5380 LAMP1-SBP- mCherry-FIREtag
Plasmid#216725PurposeExpresses LAMP1-mCherry-FIREtag in mamallian cellsDepositorInsertLAMP-1 (LAMP1 Human)
UseTagsFIREtag, Streptavidin Binding Protein (SBP), and …ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
SpCas9 TadCBEd-V106W
Plasmid#193839PurposeExpress TadCBEd-V106W (with SpCas9) in mammalian cellsDepositorInsertTadA-CDd V106W-SpCas9 D10A-2xUGI
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-hA3B-BE3
Plasmid#113411PurposeExpresses hA3B-BE3 in mammalian cellsDepositorInserthA3B-BE3 (APOBEC3B Human, S. pyogenes and Bacteriophage PBS2)
UseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV-hA3A-BE3
Plasmid#113410PurposeExpresses hA3A-BE3 in mammalian cellsDepositorInserthA3A-BE3 (APOBEC3A Human, S. pyogenes and Bacteriophage PBS2)
UseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
AAVi U6_gRNA CMV_SadCas9-KRAB
Plasmid#214609PurposeAll-in-one AAVi plasmid expressing S. aureus dCas9-KRAB with sgRNA cassetteDepositorInsertsgRNA
SadCas9
UseAAVTagsNP NLS and SV40 NLSExpressionMammalianMutationD10A, N580APromoterCMV and U6Available sinceMarch 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
ABE8.8-m
Plasmid#136294Purposeexpresses ABE8.8-m in mammalian cellsDepositorInsertABE8.8-m
UseTagsC-terminal BPNLSExpressionMammalianMutationD10A in S. pyogenes Cas9, TadA mutations describe…PromoterAvailable sinceMarch 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA GNSTM-3-Flag-10-Lamp2b-HA
Plasmid#71293PurposeEncodes (N to C): GNSTM glycosylation motif, 3 residue spacer, Flag tag,10 residue spacer, Lamp2b (exosomal transmembrane protein), 3 residue spacer, HA tag.DepositorInsertLamp2b (LAMP2 Human)
UseTagsFLAG, GNSTM glycosylation motif, HA, and Lamp2 si…ExpressionMammalianMutationPromoterCMVAvailable sinceDec. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
ACBE
Plasmid#204607PurposeMammalian expression of adenine-to-cytosine base editor ACBEDepositorInsertbpNLS-N terminal of nSpCas9-linker-mAAG (R165E/Y179F)-TadA-8e-linker-C terminal of nSpCas9-bpNLS-P2A-GFP
UseCRISPRTags6*HisExpressionMammalianMutationmAAG (R165E/Y179F); S. pyogenes Cas9 (D10A)PromoterAvailable sinceAug. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
Cbh_v5 AAV-ABE N-terminal
Plasmid#137177PurposeAAV genome: expresses the N-terminal of v5 AAV-ABE from the Cbh promoterDepositorInsertv5 AAV-ABE N-terminal
UseAAVTagsExpressionMutationCas9 D10APromoterCbhAvailable sinceJan. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
LAMP1-mCherry-FRB*
Plasmid#207145PurposeLysosome-targeted rapalog dimerizerDepositorInsertLAMP1 (LAMP1 Human)
UseTagsFRB* (T2098L mutant of FKBP-rapamycin binding dom…ExpressionMammalianMutationSilent mutationPromoterCMVAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
Integrin β4-GFP
Plasmid#205092PurposeExpress GFP-tagged human integrin β4 in mammalian cellsDepositorInsertIntegrin beta 4 (ITGB4 Human)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceSept. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pYPQ-CBE-Cas9n-Act3.0
Plasmid#178955PurposeIt consists of a Cas9 nickase (D10A) fused with a cytidine deaminase (A3A/Y130F) and MS2-SunTag-activators (ScFv-sfGFP-2xTAD), enabling simultaneous C to T conversion and gene activation.DepositorInserthA3A-zCas9-UGI-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceMay 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNTI647 dCas9-Mxi1 TetR KanMX
Plasmid#139474PurposeIntegrates CRISPRi effector and tet-responsive regulatorDepositorInsertsdCas9-Mxi1
Tet Repressor
UseCRISPRTagsMxi1 and NLSExpressionYeastMutationD10A, H840APromoterpGPM1 and pTEF1Available sinceNov. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
AXBEv2
Plasmid#204606PurposeMammalian expression of evolved adenine transversion base editor AXBEv2DepositorInsertbpNLS-TadA-8e-nSpCas9-mAAG (R165E/Y179F)-bpNLS-P2A-GFP
UseCRISPRTags6*HisExpressionMammalianMutationmAAG (R165E/Y179F); S. pyogenes Cas9 (D10A)PromoterAvailable sinceAug. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pdCas9::BFP-humanized
Plasmid#44247PurposeExpression of a catalytically inactive, human codon-optimized Cas9-BFP fusion under the control of Murine Stem Cell retroVirus LTR promoter for mammalian gene knockdownDepositorInsertdCas9 fused to BFP
UseCRISPR and RetroviralTags2xNLS & tagBFPExpressionMammalianMutationD10A H840A (catalytically inactive)PromoterMSCV LTR promoterAvailable sinceApril 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1a-LAMP-1-mGFP-IRES-Puromycin
Plasmid#134868PurposeExpresses EGFP-tagged lysosomal markerDepositorInsertLysosomal-associated membrane protein 1 (LAMP1 Human)
UseLentiviralTagsGFPExpressionMammalianMutationPromoterEF1aAvailable sinceMarch 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
ABE8.20-m
Plasmid#136300Purposeexpresses ABE8.20-m in mammalian cellsDepositorInsertABE8.20-m
UseTagsC-terminal BPNLSExpressionMammalianMutationD10A in S. pyogenes Cas9, TadA mutations describe…PromoterAvailable sinceMarch 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
miniCGBE1 (pBM1219)
Plasmid#140253PurposeCMV promoter expression plasmid for rAPOBEC1(R33A)-nCas9-P2A-EGFPDepositorInsertBE4max(R33A)-ΔUGI
UseTagsExpressionMammalianMutationR33A in rAPOBEC1, D10A in Cas9PromoterCMVAvailable sinceAug. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti-ABERA-P2A-Puro
Plasmid#112675PurposeLentivirus for constitutive expression of ABE7.10 RA (codon optimized)DepositorInsertABERA
UseLentiviralTagsFLAGExpressionMutationD10APromoterEF1sAvailable sinceJuly 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
Fuw-dCas9-dead Tet1CD-P2A-BFP
Plasmid#108246PurposeLentiviral vector to express dCas9-dead Tet1CD-P2A-tagBFPDepositorInsertdCas9-dead Tet1CD-HA-P2A-BFP
UseLentiviralTagsHAExpressionMammalianMutationdCas9-D10A, dCas9-H840A, Tet1CD-H1672Y, Tet1CD-D1…PromoterUbcAvailable sinceJune 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti PGK mKate2-OGT WT (Puro)
Plasmid#154290PurposeLentiviral vector encoding full length mKate2-2xFLAG-OGT (full length human O-GlcNAc transferase, WT)DepositorInsertO-GlcNAc Transferase (OGT Human)
UseLentiviralTags2x FLAG and mKate2ExpressionMammalianMutationWTPromoterPGKAvailable sinceDec. 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNAZeo(-) LAMP2C
Plasmid#89342PurposeExpresses human LAMP-2C in mammalian cellsDepositorInsertLysosomal-associated membrane protein 2C (LAMP2 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-dCas9-HA
Plasmid#61355Purposeencodes c-terminal HA-tagged S. pyogenes dCas9 driven by CMV promoterDepositorInsertS.pyogenes dCas9 with c-terminal HA tag
UseCRISPR and Synthetic BiologyTagsFlag, HA, and SV40 NLSExpressionMammalianMutationD10A; H840A in S.pyogenes Cas9PromoterCMVAvailable sinceApril 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
PB-CAG-DDdCas9VPH-T2A-GFP-IRES-Neo
Plasmid#102886PurposePiggyBac transposon system construct with constitutive CAG promoter expression of TMP inducible DDdCas9VP192-p65-HSF1 activator followed by T2A-EGFP as a reporter and IRES-Neo as selectionDepositorInsertDDdCas9VP192-p65-HSF1-T2A-GFP-IRES-Neo
UseCRISPRTagsExpressionMammalianMutationD10A, H840APromoterCAGAvailable sinceJuly 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMRX-IP/SECFP-hATG16A1
Plasmid#58994PurposeExpresses SECFP-hATG16A1 in mammalian cellsDepositorInsertHomo sapiens autophagy related 16-like 1 (S. cerevisiae) (ATG16L1 Human)
UseRetroviralTagsSECFPExpressionMutationPromoterAvailable sinceAug. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJMP1
Plasmid#79873PurposeBacillus subtilis dCas9 expression vector; integrates into lacA/ganADepositorInsertdCas9
UseCRISPRTagsExpressionBacterialMutationD10A, H840APromoterxylAAvailable sinceOct. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
PTPRC-bio-His
Plasmid#51813PurposeExpresses full-length Leukocyte common antigen precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertPTPRC (PTPRC Human)
UseTagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
STIM1-EGFP
Plasmid#210370PurposeExpresses STIM1 fused with EGFP in mammalian cellsDepositorInsertSTIM1 (STIM1 Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceDec. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
SpRY dCas9 KRAB
Plasmid#188501PurposeExpresses FLAG tagged SpRY dCas9 KRABDepositorInsertdCas9
UseCRISPR and LentiviralTagsExpressionMammalianMutationD10A/A61R/H840A/L1111R/D1135L/S1136W/G1218K/E1219…PromoterEF1aAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA Flag Lamp2b-HA
Plasmid#71292PurposeFlag tag with 3 residue spacer fused to the N-terminus of Lamp2b, a transmembrane exosomal protein. 3 residue spacer then HA tag fused to the C-terminus of Lamp2b.DepositorInsertLamp2b (LAMP2 Human)
UseTagsFlag, HA, and Lamp2 signal peptideExpressionMammalianMutationPromoterCMVAvailable sinceDec. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
LLP339 pGK-dCas9-SunTag
Plasmid#100943PurposedCas9 vector with SunTagx10, driven by pGK, with 3xHA tag, Puro driven by EF1aDepositorInsertdCas9, SunTag array (10xGCN4)
UseCRISPRTags3xHAExpressionMammalianMutationD10A, H840A for dCas9PromoterAvailable sinceJune 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR-aBEST
Plasmid#131464PurposeExpression of a Streptomyces codon optimized Cas9n-tadA fusion protein in pGM1190. A to G base editor for actinomycetesDepositorInsertsStreptomyces codon optimized spCas9n (D10A)
streptomyces codon optimized tadA
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromotertipAAvailable sinceNov. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV-hA3G-BE3
Plasmid#113415PurposeExpresses hA3G-BE3 in mammalian cellsDepositorInserthA3G-BE3 (APOBEC3G Human, S. pyogenes and Bacteriophage PBS2)
UseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
hu-TLR4 cDNA wt pDEST40
Plasmid#42646Purposedestination vector for expression of human TLR4 in mammalian cellsDepositorInsertTLR4 (TLR4 Human)
UseTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceMarch 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
3xFLAG-dCas9/pCMV-7.1
Plasmid#47948PurposeExpresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.DepositorInsert3xFLAG-dCas9
UseCRISPRTags3xFLAG and NLSExpressionMammalianMutationhuman codon-optimized, D10A + H840A (catalyticall…PromoterCMVAvailable sinceSept. 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCMV-hA3H-BE3
Plasmid#113416PurposeExpresses hA3H-BE3 in mammalian cellsDepositorInserthA3H-BE3 (APOBEC3H Human, S. pyogenes and Bacteriophage PBS2)
UseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDicAID_nCas9-PmCDA_NptII_Della
Plasmid#91694PurposeDicot Target-AID vector expressing dicot-optimized nCas9-PmCDA1 with sgRNA targeting SlDellaDepositorInsertSpCas9
UseTagsPmCDA1ExpressionPlantMutationD10A for nickase Cas9PromoterPcUbiAvailable sinceJune 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSI-Target-AID-NG
Plasmid#119861PurposeExpresses Target-AID-NG (NLS-nSpCas9-NG-NLS-SH3-3xFLAG-PmCDA1-NLS-UGI) in mammalian cells.DepositorInsertnSpCas9-NG (D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R)
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYPQ-CBE-SpRYn-Act3.0
Plasmid#178958PurposeIt consists of a SpRY nickase (D10A) fused with a cytidine deaminase (A3A/Y130F) and MS2 -SunTag-activators (ScFv-sfGFP-2xTAD), enabling simultaneous C to T conversion and gene activation.DepositorInserthA3A-SpRY-UGI-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceMay 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Target-ACEmax
Plasmid#139105PurposeMammalian Target-ACEmax expression vector (pSI-625)DepositorInsertBi_partite_NLS-TadA*-XTEN_linker-TadA-XTEN_linker-SpCas9(D10A)-SH3_linker-PmCDA1-UGI-SV40NLS
UseCRISPRTagsExpressionMammalianMutationPromoterCMV promoterAvailable sinceJune 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_pCMV-nCas-PmCDA1-ugi pH1-gRNA(HPRT)
Plasmid#79620PurposeExpresses nCas9-PmCDA1-UGI and gRNA(HPRT) in mammalian cellsDepositorInsertSpCas9
UseTagsPmCDA1 and UGIExpressionMammalianMutationD10A for nickase Cas9PromoterpCMVAvailable sinceJuly 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
ABE8.20-d
Plasmid#136301Purposeexpresses ABE8.20-d in mammalian cellsDepositorInsertABE8.20-d
UseTagsC-terminal BPNLSExpressionMammalianMutationD10A in S. pyogenes Cas9, TadA mutations describe…PromoterCMVAvailable sinceMarch 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
SpCas9 TadCBEa-V106W
Plasmid#193838PurposeExpress TadCBEa-V106W (with SpCas9) in mammalian cellsDepositorInsertTadA-CDa V106W-SpCas9 D10A
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTT3 Unc5B-FLAG
Plasmid#72195PurposeExpresses full-length Unc5b with a C-terminal FLAG tagDepositorInsertUnc5b (Unc5b Mouse)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pYPQ-ABE-SpRYn-Act3.0
Plasmid#178959PurposeIt consists of a SpRY nickase (D10A) fused with an adenine deaminase (ABE8e) and MS2-SunTag-activators (ScFv-sfGFP-2xTAD), enabling simultaneous A to G conversion and gene activation.DepositorInsertABE8e-SpRY-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceMay 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEX-SP-YFP-STIM1(23-685)
Plasmid#18857DepositorInsertStromal interaction molecule 1 (STIM1 Human)
UseTagsYFPExpressionMammalianMutationYFP is inserted between residues 1-23 (signal pep…PromoterAvailable sinceOct. 15, 2008AvailabilityAcademic Institutions and Nonprofits only -
miniCGBE1-NG (pBM1182)
Plasmid#140257PurposeCMV promoter expression plasmid for rAPOBEC1(R33A)-nCas9_NG-P2A-EGFPDepositorInsertBE4max(R33A)_NG-ΔUGI
UseTagsExpressionMammalianMutationR33A in rAPOBEC1, NG mutations in SpCas9, D10A in…PromoterCMVAvailable sinceAug. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pENTR1A mKate2-hu_ncOGT K842M
Plasmid#154284PurposeEntry vector encoding mKate2-2xFLAG-OGT (O-GlcNAc Transferase; K842M variant, catalytic dead)DepositorInsertO-GlcNAc Transferase (OGT Human)
UseGateway entry vectorTags2x FLAG and mKate2ExpressionMutationK842M mutation (Catalytic Inactive)PromoterAvailable sinceNov. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pX330-Flag-deSpCas9
Plasmid#92114PurposeExpression plasmid for human codon-optimized dead/inactive increased fidelity eSpCas9 (1.1) (without U6-sgRNA coding sequence)DepositorInsertdead/inactive eSpCas9 with FLAG tag
UseCRISPRTags3xFLAG and NLSExpressionMammalianMutationD10A, H840A, K848A, K1003A, R1060APromoterCbhAvailable sinceOct. 31, 2017AvailabilityAcademic Institutions and Nonprofits only