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Showing: 841 - 870 of 2257 results

  3. pBiex PKC - Peptide 8 GFP RFP

    Plasmid
    #83564
    Purpose
    Expresses a GFP-RFP FRET sensor containing the catalytic domain of PKCalpha and a short peptide substrate derived from the EGF receptor.
    Depositor
    Sivaraj Sivaramakrishnan
    Article
    Sommese et al J Biol Chem. 2016 Aug 23. pii: jbc.M116.
    Insert
    PKC alpha (PRKCA Human)
    Use
    Tags
    10 nm ER/K Helix, FLAG purification tag, TEV Prot…
    Expression
    Bacterial and Insect
    Mutation
    Only catalytic domain of PKC (aa 335-672)
    Promoter
    T7 Promoter
    Available since
    Nov. 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    83564-plasmid-map-sequence-id-166406

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  4. pBiex PKC - Peptide 8 mCit mCer

    Plasmid
    #83563
    Purpose
    Expresses a mCitrine-mCerulean FRET sensor containing the catalytic domain of PKCalpha and a short peptide substrate derived from the EGF receptor.
    Depositor
    Sivaraj Sivaramakrishnan
    Article
    Sommese et al J Biol Chem. 2016 Aug 23. pii: jbc.M116.
    Insert
    PRKCA (PRKCA Human)
    Use
    Tags
    10 nm ER/K Helix, FLAG purification tag, TEV Prot…
    Expression
    Bacterial and Insect
    Mutation
    Only catalytic domain of PKC (aa 335-672)
    Promoter
    T7 Promoter
    Available since
    Nov. 3, 2016
    Availability
    Academic Institutions and Nonprofits only
    83563-plasmid-map-sequence-id-159470

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  5. pBiex PKC - Peptide 14 mCit mCer

    Plasmid
    #83565
    Purpose
    Expresses a mCitrine-mCerulean FRET sensor containing the catalytic domain of PKCalpha and a short peptide substrate derived from the pseudosubstrate of PKCalpha.
    Depositor
    Sivaraj Sivaramakrishnan
    Article
    Sommese et al J Biol Chem. 2016 Aug 23. pii: jbc.M116.
    Insert
    PKC alpha (PRKCA Human)
    Use
    Tags
    10 nm ER/K Helix, FLAG Purificaiton Tag, Tev Prot…
    Expression
    Bacterial and Insect
    Mutation
    Only catalytic domain of PKC (aa 335-672)
    Promoter
    T7 Promoter
    Available since
    Nov. 3, 2016
    Availability
    Academic Institutions and Nonprofits only
    83565-plasmid-map-sequence-id-159510

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  20. LV_EF1a_FLAG-Mkk7-JNK2-P2A-Hygro_Barcode

    Plasmid
    #170228
    Purpose
    Barcoded lentiviral vector to express Mkk7-JNK2 fusion in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq
    Depositor
    Prashant Mali
    Article
    Parekh et al iScience. 2021 Sep 20;24(10):103149. doi
    Insert
    Map2k7-MAPK9 fusion (MAPK9, Map2k7 Human, Mouse)
    Use
    Lentiviral
    Tags
    FLAG
    Expression
    Mutation
    Fusion
    Promoter
    EF1a
    Available since
    Sept. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    170228-plasmid-map-sequence-id-334409

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  23. pNJP

    Plasmid
    #115494
    Purpose
    Expression vector for NLS-iRFP-NLS, JNK KTR-mCherry, and mKO-MK2 in mammalian cells
    Depositor
    Kazuhiro Aoki
    Article
    Miura et al Cell Rep. 2018 Sep 4;24(10):2658-2668. d
    Insert
    NLS-iRFP-NLS-P2A-JNK KTR-mCherry-P2A-mKO-MK2 (MAPK14, MAPK8 Synthetic, Human, Mouse)
    Use
    Tags
    iRFP, mCherry, and mKO
    Expression
    Mammalian
    Mutation
    mCherry S227F, mCherry G229R, mKO V211G
    Promoter
    CAG
    Available since
    Dec. 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    115494-plasmid-map-sequence-id-231521

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  25. mU6-sgAmpk1-hU6-sgAmpk2

    Plasmid
    #177224
    Purpose
    Expresses Ampk1 and Ampk2 targeting gRNAs and Cre-recombinase
    Depositor
    Monte Winslow
    Insert
    sgPrkaa1/sgPrkaa2
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    mU6/hU6
    Available since
    Jan. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    177224-plasmid-map-sequence-id-351957

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  26. pMT3 p38

    Plasmid
    #12658
    Depositor
    John Kyriakis
    Insert
    p38alpha (MAPK14 Human)
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 21, 2006
    Availability
    Academic Institutions and Nonprofits only
    12658-plasmid-map-sequence-id-169265

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  27. pEBG-hPKAalpha-Flag

    Plasmid
    #185381
    Purpose
    For mammalian expression of human GST-PKAalpha-FLAG
    Depositor
    Ayaz Najafov
    Insert
    PRKACA (PRKACA Human)
    Use
    Tags
    FLAG and GST
    Expression
    Mammalian
    Mutation
    WT
    Promoter
    Available since
    June 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    185381-plasmid-map-sequence-id-363831

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  28. bU6-sgNeo1-mU6-sgAmpk1-hU6-sgAmpk2

    Plasmid
    #177233
    Purpose
    Expresses Neomycin (bU6), Ampk1 (mU6) and Ampk2(hU6) gRNAs and Cre-recombinase
    Depositor
    Monte Winslow
    Insert
    sgNeo1/sgPrkaa1/sgPrkaa2
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    bU6/mU6/hU6
    Available since
    Jan. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    177233-plasmid-map-sequence-id-352662

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  29. pX459-puro-hPKAalpha

    Plasmid
    #185379
    Purpose
    For mammalian expression of guide RNA: caccgTTTGAACGAATCAAGACCCT that targets human PKA subunit alpha
    Depositor
    Ayaz Najafov
    Insert
    PRKACA (PRKACA Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    WT
    Promoter
    Available since
    June 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    185379-plasmid-map-sequence-id-363450

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  30. pcDNA5-FRT-TO-hPKAalpha-Flag

    Plasmid
    #185380
    Purpose
    For mammalian expression of human PKAalpha-FLAG
    Depositor
    Ayaz Najafov
    Insert
    PRKACA (PRKACA Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    WT
    Promoter
    Available since
    June 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    185380-plasmid-map-sequence-id-363613

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Showing: 841 - 870 of 2257 results