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Showing: 561 - 580 of 737 results

  1. pCRISPaint-mNeon-F-Ova-PuroR [M1G]

    Plasmid
    #171804
    Purpose
    Universal donor plasmid for CRISPitope method encoding mNeon fluorescent protein, FLAG tag, Ovalbumin CD8+ T cell epitope [AA: 257-264 (SIINFEKL)] and puromycin resistance cassette [p.M1G]
    Depositor
    Michael Hoelzel
    Article
    Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.
    Insert
    mNeonGreen-FLAG-Ova-T2A-PuroR
    Use
    Gene tagging
    Tags
    Expression
    Mutation
    PuroR: Changed Methionin 1 to Glycine.
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    171804-plasmid-map-sequence-id-337975

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  2. pCRISPaint-mNeon-F-Ova-BlastR [M1G]

    Plasmid
    #171808
    Purpose
    Universal donor plasmid for CRISPitope method encoding mNeon fluorescent protein, FLAG tag, Ovalbumin CD8+ T cell epitope [AA: 257-264 (SIINFEKL)] and blasticidin resistance cassette [p.M1G
    Depositor
    Michael Hoelzel
    Article
    Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.
    Insert
    mNeonGreen-F-Ova-T2A-BlastR
    Use
    Gene tagging
    Tags
    Expression
    Mutation
    BlastR: Changed Methionin 1 to Glycine.
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    171808-plasmid-map-sequence-id-337980

    Enlarge

  3. pCRISPaint-mScarlet-F-Ova-PuroR [M1G]

    Plasmid
    #171813
    Purpose
    Universal donor plasmid for CRISPitope method encoding mScarlet fluorescent protein, FLAG tag, Ovalbumin CD8+ T cell epitope [AA: 257-264 (SIINFEKL)] and puromycin resistance cassette [p.M1G]
    Depositor
    Michael Hoelzel
    Article
    Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.
    Insert
    mScarlet-F-Ova-T2A-PuroR
    Use
    Gene tagging
    Tags
    Expression
    Mutation
    PuroR: Changed Methionin 1 to Glycine.
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    171813-plasmid-map-sequence-id-338044

    Enlarge

  4. pCRISPaint-mScarlet-F-Ova-BlastR [M1G]

    Plasmid
    #171817
    Purpose
    Universal donor plasmid for CRISPitope method encoding mScarlet fluorescent protein, FLAG tag, Ovalbumin CD8+ T cell epitope [AA: 257-264 (SIINFEKL)] and blasticidin resistance cassette [p.M1G]
    Depositor
    Michael Hoelzel
    Article
    Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.
    Insert
    mScarlet-F-Ova-T2A-BlastR
    Use
    Gene tagging
    Tags
    Expression
    Mutation
    BlastR: Changed Methionin 1 to Glycine.
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    171817-plasmid-map-sequence-id-338126

    Enlarge

  12. eGFP-ova-C1

    Plasmid
    #163524
    Purpose
    Expresses both eGFP and chicken ovalbumin in mammalian cells
    Depositor
    Thomas Weber
    Insert
    enhanced-GFP-chicken ovalbumin tandem
    Use
    Tags
    eGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    163524-plasmid-map-sequence-id-321577

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  14. pET15b/GVcl 1-1066 /*TBM

    Plasmid
    #162781
    Purpose
    amplification of Gallus gallus Vcl mutated in Tankyrase Binding Domain II
    Depositor
    Laura Lafon-Hughes
    Article
    et al PeerJ
    Insert
    Gallus gallus vinculin (VCL Chicken)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changed Gly 454 to Val
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    162781-plasmid-map-sequence-id-321530

    Enlarge

  15. Tol2-GgVCL/WT

    Plasmid
    #162787
    Purpose
    CONTROL_transfection of mammalian cells with WT Vcl under a β-actin promoter, with IRES-GFP allowing identification of transfected cells
    Depositor
    Laura Lafon-Hughes
    Article
    et al PeerJ
    Insert
    Gallus gallus vinculin (VCL Chicken)
    Use
    Tags
    IRES-GFP
    Expression
    Bacterial and Mammalian
    Mutation
    WT
    Promoter
    beta actin
    Availability
    Academic Institutions and Nonprofits only
    162787-plasmid-map-sequence-id-327628

    Enlarge

  16. Tol2-GgVCL/*TBM

    Plasmid
    #162790
    Purpose
    MUTATED_transfection of mammalian cells with Vcl mutated in Tankyrase Binding Motif -II (TBM-II) under a β-actin promoter, with IRES-GFP allowing identification of transfected cells
    Depositor
    Laura Lafon-Hughes
    Article
    et al PeerJ
    Insert
    Mutated Gallus gallus vinculin (G454V) (VCL Chicken)
    Use
    Tags
    IRES-GFP
    Expression
    Bacterial and Mammalian
    Mutation
    changed Gly 454 to Val
    Promoter
    beta actin
    Availability
    Academic Institutions and Nonprofits only
    162790-plasmid-map-sequence-id-321532

    Enlarge

  20. AAV-EF1a-jGCaMP8m-WPRE

    Plasmid
    #176757
    Purpose
    AAV-mediated expression of GCaMP8m under the EF1a promoter
    Depositor
    Loren Looger
    Has Service
    AAV Retrograde, AAV1, and AAV9
    Insert
    jGCaMP8m
    Use
    AAV
    Tags
    6xHis
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Availability
    Academic Institutions and Nonprofits only
    176757-plasmid-map-sequence-id-351328

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Showing: 561 - 580 of 737 results