Skip to main content
Addgene
Showing: 561 - 580 of 742 results

  7. pCKC764

    Plasmid
    #226625
    Purpose
    piggyBAC pCAG-hCas9-T2A-Unbiased3
    Depositor
    Chang Liu
    Article
    Carlson et al bioRxiv [Preprint]. 2024 Jun 11:2024.06.
    Inserts
    CAG promoter, SpCas9, T2A, Unbiased3 variant of TdT (DNTT Chicken, Human, S. pyogenes)
    EF1a promoter, blasticidin resistance
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV and EF1a
    Available since
    Oct. 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    226625-plasmid-map-sequence-id-449160

    Enlarge

  10. pGP-JFRC59-13XLexAop2-IVS-Syn21-RSET-jGCaMP8f-p10

    Plasmid
    #218867
    Purpose
    LexA activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogaster
    Depositor
    GENIE Project
    Article
    Reagents et al figshare Dataset. 2024. doi: 10.6084/m9.
    Insert
    RSET-jGCaMP8f
    Use
    Tags
    6xHis-T7 gene 10 leader-Xpress epitope-Enterokina…
    Expression
    Insect
    Mutation
    Q315L
    Promoter
    hsp70
    Available since
    May 22, 2024
    Availability
    Academic Institutions and Nonprofits only
    218867-plasmid-map-sequence-id-434263

    Enlarge

  12. pcDNA3.1-VcnTS-E1015A

    Plasmid
    #213415
    Purpose
    Vinculin tension sensor (VcnTS) with a single directionally asymmetric, force strengthening (DAFS) variant point mutation (E1015A).
    Depositor
    Brenton Hoffman
    Article
    Chirasani et al Nat Commun. 2023 Dec 14;14(1):8300. doi:
    Insert
    VcnTS-E1015A (VCL Synthetic, Chicken)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Mutated vinculin glutamic acid 1015 to alanine (E…
    Promoter
    CMV
    Available since
    Feb. 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    213415-plasmid-map-sequence-id-422214

    Enlarge

  13. pcDNA3.1-VcnTS-E1021A

    Plasmid
    #213416
    Purpose
    Vinculin tension sensor (VcnTS) with a single directionally asymmetric, force strengthening (DAFS) variant point mutation (E1021A).
    Depositor
    Brenton Hoffman
    Article
    Chirasani et al Nat Commun. 2023 Dec 14;14(1):8300. doi:
    Insert
    VcnTS-E1021A (VCL Synthetic, Chicken)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Mutated vinculin glutamic acid 1021 to alanine (E…
    Promoter
    CMV
    Available since
    Feb. 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    213416-plasmid-map-sequence-id-422049

    Enlarge

  14. pcDNA3.1-VcnTS-E1015A-E1021A

    Plasmid
    #213417
    Purpose
    Vinculin tension sensor (VcnTS) with both directionally asymmetric, force strengthening (DAFS) variant point mutations (E1015A and E1021A).
    Depositor
    Brenton Hoffman
    Article
    Chirasani et al Nat Commun. 2023 Dec 14;14(1):8300. doi:
    Insert
    VcnTS-E1015A-E1021A (VCL Synthetic, Chicken)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Mutated vinculin glutamic acid 1015 and 1021 to a…
    Promoter
    CMV
    Available since
    Feb. 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    213417-plasmid-map-sequence-id-422213

    Enlarge

  15. pRRL-VcnTS-E1015A-E1021A

    Plasmid
    #213411
    Purpose
    Vinculin tension sensor (VcnTS) with both directionally asymmetric, force strengthening (DAFS) variant point mutations (E1015A and E1021A), in lentiviral expression vector.
    Depositor
    Brenton Hoffman
    Article
    Chirasani et al Nat Commun. 2023 Dec 14;14(1):8300. doi:
    Insert
    VcnTS-E1015A-E1021A (VCL Synthetic, Chicken)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Mutated vinculin glutamic acid 1015 and 1021 to a…
    Promoter
    CMV
    Available since
    Feb. 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    213411-plasmid-map-sequence-id-420344

    Enlarge

  16. pcDNA3.1-VcnCS-E1015A

    Plasmid
    #213412
    Purpose
    Vinculin conformation sensor (VcnCS) with a single directionally asymmetric, force strengthening (DAFS) variant point mutation (E1015A).
    Depositor
    Brenton Hoffman
    Article
    Chirasani et al Nat Commun. 2023 Dec 14;14(1):8300. doi:
    Insert
    VcnCS-E1015A (VCL Synthetic, Chicken)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Mutated vinculin glutamic acid 1015 to alanine (E…
    Promoter
    CMV
    Available since
    Feb. 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    213412-plasmid-map-sequence-id-422215

    Enlarge

  17. pcDNA3.1-VcnCS-E1021A

    Plasmid
    #213413
    Purpose
    Vinculin conformation sensor (VcnCS) with a single directionally asymmetric, force strengthening (DAFS) variant point mutation (E1021A).
    Depositor
    Brenton Hoffman
    Article
    Chirasani et al Nat Commun. 2023 Dec 14;14(1):8300. doi:
    Insert
    VcnCS-E1021A (VCL Synthetic, Chicken)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Mutated vinculin glutamic acid 1021 to alanine (E…
    Promoter
    CMV
    Available since
    Feb. 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    213413-plasmid-map-sequence-id-422114

    Enlarge

  18. pcDNA3.1-VcnCS-E1015A-E1021A

    Plasmid
    #213414
    Purpose
    Vinculin conformation sensor (VcnCS) with both directionally asymmetric, force strengthening (DAFS) variant point mutations (E1015A and E1021A).
    Depositor
    Brenton Hoffman
    Article
    Chirasani et al Nat Commun. 2023 Dec 14;14(1):8300. doi:
    Insert
    VcnCS-E1015A-E1021A (VCL Synthetic, Chicken)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Mutated vinculin glutamic acid 1015 and 1021 to a…
    Promoter
    CMV
    Available since
    Feb. 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    213414-plasmid-map-sequence-id-422048

    Enlarge

  19. pRRL-VinculinTS-S1033A

    Plasmid
    #211835
    Purpose
    Vinculin tension sensor (VinTS) with vinculin S1033 unphosphorylatable point mutation (S1033A), in lentiviral expression vector.
    Depositor
    Brenton Hoffman
    Article
    Shoyer et al Proc Natl Acad Sci U S A. 2023 Dec 12;12
    Insert
    VinculinTS-S1033A (VCL Synthetic, Chicken)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    mutated vinculin serine 1033 to alanine (S1033A),…
    Promoter
    CMV
    Available since
    Jan. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    211835-plasmid-map-sequence-id-417709

    Enlarge

  20. pRRL-VinculinTS-S1033D

    Plasmid
    #211893
    Purpose
    Vinculin tension sensor (VinTS) with vinculin S1033 phosphomimetic point mutation (S1033D), in lentiviral expression vector.
    Depositor
    Brenton Hoffman
    Article
    Shoyer et al Proc Natl Acad Sci U S A. 2023 Dec 12;12
    Insert
    VinculinTS-S1033D (VCL Synthetic, Chicken)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    mutated vinculin serine 1033 to aspartic acid (S1…
    Promoter
    CMV
    Available since
    Jan. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    211893-plasmid-map-sequence-id-419257

    Enlarge

Showing: 561 - 580 of 742 results