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pMXs-3XHA-EGFP-OMP25

Plasmid

#83356

Purpose

For tagging mitochondria with HA epitopes (HA-MITO construct)

Depositor

David Sabatini

Article

Chen et al Cell. 2016 Aug 25;166(5):1324-1337.e11.

Insert

3XHA-EGFP-OMP25

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 26, 2016

Availability

Academic Institutions and Nonprofits only

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pLX304 CMV FKBP-V5-sTurboID-high affinity (N)

Plasmid

#153004

Purpose

Expresses high-affinity split-TurboID N-terminal fragment in mammalian cells

Depositor

Alice Ting

Article

Cho et al Proc Natl Acad Sci U S A. 2020 May 18. p

Insert

sTurboID-high affinity (N) (G78)

Use

Lentiviral

Tags

FKBP and V5

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 16, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-15F11-HA-mCh

Plasmid

#129591

Purpose

The encoded protein is the anti-HA scFv (anti-HA frankenbody) fused with the mCherry. It can be used to track mature and nascent HA tagged proteins in living organism.

Depositor

Tim Stasevich

Article

Zhao et al Nat Commun. 2019 Jul 3;10(1):2947. doi:

Insert

Anti-HA frankenbody-mCherry (15F11-HA scFv-mCh)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 15, 2019

Availability

Academic Institutions and Nonprofits only

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Mfn2-Myc

Plasmid

#23213

Depositor

David Chan

Article

Chen et al J Cell Biol. 2003 Jan 20. 160(2):189-200

Insert

Mfn2 (Mfn2 Mouse)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 24, 2010

Availability

Academic Institutions and Nonprofits only

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pCTcon2

Plasmid

#41843

Depositor

Dane Wittrup

Article

Chao et al Nat Protoc. 2006;1(2):755-68.

Insert

CD20

Use

Tags

HA and c-myc

Expression

Yeast

Mutation

Promoter

Available since

Jan. 23, 2013

Availability

Academic Institutions and Nonprofits only

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pDECKO_mCherry

Plasmid

#78534

Purpose

Backbone to clone single or paired sgRNAs

Depositor

Rory Johnson, Roderic Guigo

Article

Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6 (for expressing sgRNA)

Available since

July 12, 2016

Availability

Academic Institutions and Nonprofits only

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Mfn1-Myc

Plasmid

#23212

Depositor

David Chan

Article

Chen et al J Cell Biol. 2003 Jan 20. 160(2):189-200

Insert

Mfn1 (Mfn1 Mouse)

Use

Tags

6xHis, 9xMyc, and Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 28, 2010

Availability

Academic Institutions and Nonprofits only

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pMP31-1

Plasmid

#45905

Depositor

Harmit Malik

Article

Patel et al PLoS Biol. 2012;10(3):e1001282. doi: 10.

Insert

mitochondrial antiviral signaling protein (MAVS Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 19, 2013

Availability

Academic Institutions and Nonprofits only

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pSANG10-3F-BG4

Plasmid

#55756

Purpose

Expresses BG4 scFv anti-G-quadruplex antibody in bacteria

Depositor

Shankar Balasubramanian

Article

Biffi et al Nat Chem. 2013 Mar;5(3):182-6. doi: 10.1

Insert

BG4

Use

Tags

6xHis, pelB leader, and tri-FLAG

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 11, 2014

Availability

Academic Institutions and Nonprofits only

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pBabe-Puro-IKBalpha-mut (super repressor)

Plasmid

#15291

Depositor

William Hahn

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

inhibitor of Kappa B alpha (NFKBIA Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

S32A/S36A: “super-repressor” allele (resistant to…

Promoter

Available since

July 12, 2007

Availability

Academic Institutions and Nonprofits only

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pCS2-UTX-F

Plasmid

#17438

Purpose

Expression of UTX in mammalian cells

Depositor

Kai Ge

Article

Hong et al Proc Natl Acad Sci U S A. 2007 Nov 20. 1

Insert

UTX (KDM6A Human)

Use

Tags

6xMyc and Flag

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 19, 2008

Availability

Academic Institutions and Nonprofits only

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cEF.tk-GFP

Plasmid

#33308

Depositor

Linzhao Cheng

Article

Pomper et al Cell Res. 2009 Mar;19(3):370-9.

Insert

HSV-TK/GFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

March 29, 2012

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1-Nanog

Plasmid

#28221

Depositor

Linzhao Cheng

Article

Chou et al Cell Res. 2011 Jan 18. ():.

Insert

Nanog (NANOG Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 2, 2011

Availability

Academic Institutions and Nonprofits only

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pEGFP-SF2

Plasmid

#17990

Depositor

Tom Misteli

Article

Phair et al Nature. 2000 Apr 6. 404(6778):604-9.

Insert

SF2 (SRSF1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

contains SF2 (aa1-210)

Promoter

Available since

May 13, 2008

Availability

Academic Institutions and Nonprofits only

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Cyto-roGFP

Plasmid

#49435

Purpose

Expresses thiol redox-sensitive ratiometric sensor roGFP in cytosol of mammalian cells

Depositor

Paul Schumacker

Article

Waypa et al Circ Res. 2010 Feb 19;106(3):526-35. doi

Insert

roGFP2

Use

Adenoviral

Tags

Expression

Mammalian

Mutation

C48S, Q80R, S147C, and Q204C in Clontech's G…

Promoter

CMV

Available since

Dec. 9, 2013

Availability

Academic Institutions and Nonprofits only

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lentiCRISPRv2 hygro

Plasmid

#98291

Purpose

Variant of lentiCRISPRv2 that confers hygromycin resistance

Depositor

Brett Stringer

Article

Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

EF-1a

Available since

Aug. 16, 2017

Availability

Academic Institutions and Nonprofits only

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mRFP-Clc

Plasmid

#14435

Depositor

Ari Helenius

Article

Tagawa et al J Cell Biol. 2005 Aug 29. 170(5):769-79.

Insert

Clathrin light chain (Clc Mouse)

Use

Tags

mRFP

Expression

Mammalian

Mutation

Promoter

Available since

March 23, 2007

Availability

Academic Institutions and Nonprofits only

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pBPGAL4.2Uw-2

Plasmid

#26227

Depositor

Gerald Rubin

Article

Pfeiffer et al Genetics. 2010 Aug 9. ():.

Insert

GAL4 (GAL4 Budding Yeast)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Oct. 7, 2010

Availability

Academic Institutions and Nonprofits only

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pCFJ151 - ttTi5605_MCS

Plasmid

#19330

Depositor

Erik Jorgensen

Article

Christian et al

Insert

ttTi5605 targeting region

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

Oct. 29, 2008

Availability

Academic Institutions and Nonprofits only

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pSicoR p53

Plasmid

#12090

Depositor

Tyler Jacks

Article

Ventura et al Proc Natl Acad Sci U S A. 2004 Jul 13. 1

Insert

p53 shRNA (Trp53 Mouse)

Use

Cre/Lox, Lentiviral, and RNAi

Tags

Expression

Mammalian

Mutation

EGFP is expressed from this plasmid as a marker, …

Promoter

Available since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

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