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Showing: 5761 - 5780 of 6923 results

  1. IBMc180

    Plasmid
    #161942
    Purpose
    Carries Golden Gate substitution insert for acVHH-assisted bisection mapping
    Depositor
    Baojun Wang
    Article
    Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:
    Insert
    pSB1A3-BbsI-10aa-acVHH-T-cat-PBAD(SapI)-RiboJ-BCD22-acVHH-10aa-SapI
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PBAD
    Available since
    March 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    161942-plasmid-map-sequence-id-329042

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  2. IBMc367

    Plasmid
    #161947
    Purpose
    Contains Type IIS restriction site free elements for IBM staging vectors. Additional BsaI site to facilitate size selection. Staging plasmid for IBM on ECF20
    Depositor
    Baojun Wang
    Article
    Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:
    Insert
    pSB1C3(BsmBI-,BsaI+)-BsaI-ECF20(6-188)-BsaI_v3
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    161947-plasmid-map-sequence-id-326355

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  3. IBMc228

    Plasmid
    #161946
    Purpose
    Contains Type IIS restriction site free elements for IBM staging vectors. Additional BsaI site to facilitate size selection. Staging plasmid for IBM on TetR
    Depositor
    Baojun Wang
    Article
    Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:
    Insert
    pSB1C3(BsmBI-,BsaI+)-BsaI-TetR(20-188)-BsaI
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    161946-plasmid-map-sequence-id-326343

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  4. pJK528

    Plasmid
    #155388
    Purpose
    dCas12a oscillator, reverse crRNAs + PA4-mVenus. (Integration of 511; pOSIP)
    Depositor
    Pamela Silver
    Article
    Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81
    Inserts
    dCas12a (F. novicida)
    PA4-mVenus
    dCas12a oscillator crRNAs
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    D917A (nuclease-deactivating)
    Promoter
    Available since
    Feb. 12, 2021
    Availability
    Academic Institutions and Nonprofits only
    155388-plasmid-map-sequence-id-324484

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  5. pJK434

    Plasmid
    #155378
    Purpose
    PphlF_A4NT, 1x cascade + [dCas9 + PA4-mVenus]. (Integration of 429; pOSIP)
    Depositor
    Pamela Silver
    Article
    Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81
    Inserts
    dCas9 (bacteria)
    PA4-mVenus
    PphlF_A4NT in dCas9 sgRNA oscillator v1
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    D10A H840A (catalytically inactive)
    Promoter
    Available since
    Feb. 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    155378-plasmid-map-sequence-id-324490

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  9. pLz77_CB_operon_CsoS2_N3

    Plasmid
    #140859
    Purpose
    Same as pLz37 but with CsoS2 discontinuously truncated such that repeats N1, N2, and N4 are removed
    Depositor
    David Savage
    Article
    Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-
    Insert
    CbbL, CbbS, CsoS2 (delN1-N2, N4), CsoSCA, CsoS4A, CsoS4B, CsoS1C, CsoS1A, CsoS1B, CsoS1D
    Use
    Tags
    Expression
    Bacterial
    Mutation
    deleted amino acids 1-108 and 220-235 of CsoS2
    Promoter
    Available since
    June 19, 2020
    Availability
    Academic Institutions and Nonprofits only
    140859-plasmid-map-sequence-id-300514

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  10. pLz78_CB_operon_CsoS2_delNTD

    Plasmid
    #140860
    Purpose
    Same as pLz37 but with CsoS2 truncated to remove the entire NTD (i.e. N1, N2, N3, and N4 are removed)
    Depositor
    David Savage
    Article
    Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-
    Insert
    CbbL, CbbS, CsoS2 (delNTD), CsoSCA, CsoS4A, CsoS4B, CsoS1C, CsoS1A, CsoS1B, CsoS1D
    Use
    Tags
    Expression
    Bacterial
    Mutation
    deleted amino acids 1-235 of CsoS2
    Promoter
    Available since
    June 19, 2020
    Availability
    Academic Institutions and Nonprofits only
    140860-plasmid-map-sequence-id-300512

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Showing: 5761 - 5780 of 6923 results