Addgene

We narrowed to 18,787 results for: tes

Showing: 5741 - 5760 of 18787 results

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FU- dio Teal-gephyrin - W

Plasmid

#73918

Purpose

To express the inhibitory scaffold gephyrin tagged to teal fluorescent protein. Expression is Cre dependant. Plasmid can also be used to make lentivirus.

Depositor

Elly Nedivi

Article

Villa et al Neuron. 2016 Feb 3. pii: S0896-6273(16)0

Insert

Teal-gephyrin

Use

Lentiviral

Tags

Teal

Expression

Mammalian

Mutation

Promoter

Available since

April 15, 2016

Availability

Academic Institutions and Nonprofits only

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pScalps_Puro

Plasmid

#99636

Purpose

Empty backbone, 3rd generation lentiviral vector

Depositor

Silvia Monticelli

Article

Montagner et al Cell Rep. 2016 May 17;15(7):1566-79. doi

Type

Empty backbone

Use

Lentiviral

Tags

none

Expression

Mammalian

Mutation

Promoter

SFFVp

Available since

Aug. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pMRX-INU-TEX264-FLAG

Plasmid

#128258

Purpose

Expresses TEX264 in mammalian cells

Depositor

Noboru Mizushima

Article

Chino et al Mol Cell. 2019 Apr 11. pii: S1097-2765(1

Insert

TEX264 (TEX264 Human)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 26, 2019

Availability

Academic Institutions and Nonprofits only

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pSEVA221pmCDA1-T7RNAP

Plasmid

#167978

Purpose

Expresses fusion protein pmCDA1-T7RNAP in E. coli

Depositor

Luis Ángel Fernández

Article

Alvarez et al Nat Commun. 2020 Dec 22;11(1):6436. doi:

Insert

pmCDA1-T7RNAP (T7p07 Synthetic)

Use

Tags

T7 tag and T7RNAP

Expression

Bacterial

Mutation

Promoter

TetR-Ptet

Available since

June 17, 2021

Availability

Academic Institutions and Nonprofits only

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pTarget

Plasmid

#186452

Purpose

used as source for acquisition of new spacers

Depositor

John van der Oost

Article

Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

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pBabepuro-HA-GASC1

Plasmid

#24182

Purpose

Mammalian expression of human JMJD2C with HA tag

Depositor

Kristian Helin

Article

Cloos et al Nature. 2006 Jul 20. 442(7100):307-11.

Insert

GASC1 (KDM4C Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only
pETM-41-HsPABPC1_J

Plasmid

#146644

Purpose

Bacterial Expression of HsPABPc1

Depositor

Elisa Izaurralde

Article

Huntzinger et al EMBO J. 2010 Dec 15;29(24):4146-60. doi:

Insert

HsPABPc1 (PABPC1 Human)

Use

Tags

Expression

Bacterial

Mutation

two silent mutations compared to the sequence giv…

Promoter

Available since

Jan. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-Cas9-162A

Plasmid

#84918

Purpose

for in vitro transcription of Cas9 with polyA tail encoded

Depositor

Ralf Kuehn

Article

Chu et al BMC Biotechnol. 2016 Jan 16;16(1):4. doi

Insert

Cas9

Use

Tags

Flag, NLS, and polyA tail

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Oct. 31, 2017

Availability

Academic Institutions and Nonprofits only

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sAB-MBP

Plasmid

#204736

Purpose

synthetic antigen-binding fragment specifically recognizing bacteria maltose-binding protein

Depositor

Minglei Zhao

Article

Yu et al Nat Chem Biol. 2021 Aug;17(8):896-905. d

Insert

synthetic antigen-binding fragment recognizing bacteria maltose-binding protein

Use

Tags

Expression

Mutation

Promoter

Available since

Oct. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pRG710_lexO-HO_LexA-TF_HIS3MX

Plasmid

#154819

Purpose

Inducible expression of HO endonuclease in S. cerevisiae

Depositor

Lorraine Symington

Article

Gnugge et al Nucleic Acids Res. 2020 Oct 14. pii: 592

Inserts

HO endonuclease (HO Budding Yeast)
LexA-ER-B112

Use

Tags

FLAG

Expression

Yeast

Mutation

Promoter

ACT1 and lexO_4-CYC1_core

Available since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-FLAG-MAGE-2-429

Plasmid

#89090

Purpose

mammalian expression of full-length human MAGE-A11 with N-terminal FLAG tag

Depositor

Elizabeth Wilson

Article

Bai et al Mol Cell Biol. 2005 Feb;25(4):1238-57.

Insert

MAGE-A11 (MAGEA11 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 6, 2017

Availability

Academic Institutions and Nonprofits only
pCMV-Tag1-NS5A

Plasmid

#17646

Depositor

Xin Wang

Article

Budhu et al Carcinogenesis. 2007 Jul . 28(7):1552-60

Insert

HCV NS5A (POLY Hepatitis C virus)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

March 27, 2008

Availability

Academic Institutions and Nonprofits only

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pCEP4-YU2gp160

Plasmid

#100927

Purpose

Mammalian expression plasmid for Env from the YU-2 HIV-1 isolate

Depositor

Erik Procko

Article

Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

Insert

HIV-1 (YU2) Env

Use

Tags

CD5 leader sequence

Expression

Mammalian

Mutation

Codon-optimized synthetic gene

Promoter

CMV

Available since

March 25, 2019

Availability

Academic Institutions and Nonprofits only

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pTRIPZ TREX1-KI

Plasmid

#127701

Purpose

For knocking in TREX1 in mouse - Doxycyclin inducible

Depositor

Sandra Demaria

Article

Vanpouille-Box et al Nat Commun. 2017 Jun 9;8:15618. doi: 10.

Insert

mouse TREX1 cDNA (Trex1 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pHSE901

Plasmid

#91708

Purpose

CRISPR/Cas9-mediated base editing in dicots. Contains Cas9(D10A) under CaMV 35S promoter and empty gRNA scaffold, HygR

Depositor

Qi-Jun Chen

Article

Chen et al Sci China Life Sci. 2017 Mar 16. doi: 10

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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pET28C-mCherry-53BP1LCD

Plasmid

#204407

Purpose

E. coli expression of the 53BP1 low-complexity domain (1208-1977) with an N-terminal mCherry tag

Depositor

Samie Jaffrey

Article

Dumelie et al Nat Chem Biol. 2023 Nov 16. doi: 10.1038

Insert

53BP1(1208-1977) (TP53BP1 Human)

Use

Tags

6x-His; mCherry

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

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phR-Luc

Plasmid

#139644

Purpose

Mammalian gene expression

Depositor

Markku Varjosalo

Article

Yadav et al iScience. 2020 Feb 21;23(2):100871. doi:

Insert

Renilla luciferase

Use

Luciferase

Tags

Expression

Mutation

Promoter

SV40

Available since

May 13, 2020

Availability

Academic Institutions and Nonprofits only

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pMSCV-Flag-hOct4

Plasmid

#20072

Depositor

Juan Belmonte

Article

Aasen et al Nat Biotechnol. 2008 Nov . 26(11):1276-8

Insert

hOct4 (POU5F1 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 9, 2009

Availability

Academic Institutions and Nonprofits only

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pLV-dfROX

Plasmid

#137170

Purpose

Lentiviral transfer vector for expression of roGFP2 and HyPer Red

Depositor

Shaun McCullough

Article

Faber et al Toxicol Sci. 2020 Jun 11. pii: 5856111.

Insert

roGFP2-2A-HyPer Red

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF1a

Available since

April 30, 2021

Availability

Academic Institutions and Nonprofits only

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pCLX-CHOP-dGFP

Plasmid

#71299

Purpose

Lentiviral plasmid to express destabilized GFP under the control of the ATF-responsive CHOP promoter

Depositor

Patrick Salmon

Article

Boitard et al Cell Rep. 2015 Mar 3;10(8):1349-61. doi:

Insert

dGFP

Use

Lentiviral

Tags

Expression

Mutation

Promoter

CHOP (ATF-responsive)

Available since

March 16, 2016

Availability

Academic Institutions and Nonprofits only

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