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Plasmid#173510PurposeThis is a binary plasmid that can be transferred into Agrobacterium and used to transiently express the protein encoded by the insert in plants by agro-infiltration.DepositorInsertPITG_04089_T30-4
UseTagsExpressionPlantMutationPromoter35SAvailable sinceSept. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pICSL86977OD:PITG_04085
Plasmid#173509PurposeThis is a binary plasmid that can be transferred into Agrobacterium and used to transiently express the protein encoded by the insert in plants by agro-infiltration.DepositorInsertPITG_04085_T30-4
UseTagsExpressionPlantMutationPromoter35SAvailable sinceSept. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pICSL86977OD:PITG_02860
Plasmid#173508PurposeThis is a binary plasmid that can be transferred into Agrobacterium and used to transiently express the protein encoded by the insert in plants by agro-infiltration.DepositorInsertPITG_02860_T30-4
UseTagsExpressionPlantMutationPromoter35SAvailable sinceSept. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pICSL86977OD:PITG_02860a
Plasmid#173507PurposeThis is a binary plasmid that can be transferred into Agrobacterium and used to transiently express the protein encoded by the insert in plants by agro-infiltration.DepositorInsertPITG_02860_P17777
UseTagsExpressionPlantMutationPromoter35SAvailable sinceSept. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pICSL86977OD:PITG_00582
Plasmid#173506PurposeThis is a binary plasmid that can be transferred into Agrobacterium and used to transiently express the protein encoded by the insert in plants by agro-infiltration.DepositorInsertPITG_00582_T30-4
UseTagsExpressionPlantMutationPromoter35SAvailable sinceSept. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTM
Plasmid#162783PurposeProtein expression in CHO cells - hTPA leader and C-terminal c-myc and 10xhis tags are codedDepositorTypeEmpty backboneUseTags10xHis, c-myc, and hTPA leaderExpressionMammalianMutationPromoterCHO Elongation factor 1AAvailable sinceJuly 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMBP-HRV3C-AcrIF9
Plasmid#141442PurposeExpression of AcrIF9 in E. coli, tagged with an HRV3C-cleavage fusion to Maltose Binding Protein.DepositorInsertAcrIF9
UseTagsHRV3C cleavage site and Maltose Binding ProteinExpressionBacterialMutationPromoterptacAvailable sinceMay 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pWZL N-Myc-hPOT1_K90E-IRES_hygro
Plasmid#166407PurposeExpress hPOT1 K90E in mammalian cellsDepositorInserthPOT1 (POT1 Human)
UseTagsmycExpressionMammalianMutationK90EPromoterAvailable sinceMay 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
IBMc227
Plasmid#161948PurposeContains Type IIS restriction site free elements for expression vectors in IBM. Expression vector to generate ORF insertion library in IBM on TetRDepositorInsertpSB3A5*(BsaI-)-PBAD(SapI-)-tet1-TetR(1-20)-BsaI-BsaI-TetR(188-207)-Ter
UseTagsExpressionBacterialMutationPromoterPBADAvailable sinceMarch 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
IBMc367
Plasmid#161947PurposeContains Type IIS restriction site free elements for IBM staging vectors. Additional BsaI site to facilitate size selection. Staging plasmid for IBM on ECF20DepositorInsertpSB1C3(BsmBI-,BsaI+)-BsaI-ECF20(6-188)-BsaI_v3
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
IBMc228
Plasmid#161946PurposeContains Type IIS restriction site free elements for IBM staging vectors. Additional BsaI site to facilitate size selection. Staging plasmid for IBM on TetRDepositorInsertpSB1C3(BsmBI-,BsaI+)-BsaI-TetR(20-188)-BsaI
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMKO.1-shNuf2
Plasmid#160961PurposeNuf2 shRNA in pMKO.1 retroviral vectorDepositorInsertNuf2 shRNA (Nuf2 Mouse)
UseRNAi and RetroviralTagsExpressionMutationPromoterU6Available sinceDec. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-GFP-EBA175-Flag(P1424G)
Plasmid#155128PurposeExpresses N-terminally GFP-tagged EBA175 P1424G variant (residues 1284-1462) from pcDNA3.1DepositorInsertPfEBA175
UseTagsFlag, GFP, and signal peptide (residues 1 to 32) …ExpressionMammalianMutationP1424G; insert codes for EBA175 residues 1284 to …PromoterCMVAvailable sinceNov. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-HA-PfROM4 (H578A)
Plasmid#154858PurposeExpresses N-terminally HA-tagged PfROM4 variant H578A (codon optimized for mammalian expression) from pcDNA3.1DepositorInsertPfROM4 (PF3D7_0506900 Synthetic)
UseTagstriple HAExpressionMammalianMutationHistidine 578 changed to AlaninePromoterCMVAvailable sinceNov. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-HA-PfROM4 (G470A/G471N)
Plasmid#154859PurposeExpresses N-terminally HA-tagged PfROM4 variant G470A/G471N (codon optimized for mammalian expression) from pcDNA3.1DepositorInsertPfROM4 (PF3D7_0506900 Synthetic)
UseTagstriple HAExpressionMammalianMutationGlycine 470 changed to Alanine and Glycine 471 ch…PromoterCMVAvailable sinceNov. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-HA-PfROM1 (A144G/N145G)
Plasmid#154873PurposeExpresses N-terminally HA-tagged PfROM1 variant A144G/N145G (codon optimized for mammalian expression) from pcDNA3.1DepositorInsertPfROM1 (PF3D7_0506900 Synthetic)
UseTagstriple HAExpressionMammalianMutationAlanine 144 changed to Glycine and Asparagine 145…PromoterCMVAvailable sinceNov. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET15-His-∆NGlpG(C104A/M247C)
Plasmid#155133PurposeExpresses N-terminally His-tagged E. coli GlpG C104A/M247C variant (residues 87-276) from pET15bDepositorInsertGlpG (glpG Escherichia coli)
UseTagsHisExpressionBacterialMutationC104A/M247C, insert codes for GlpG residues 87-276PromoterT7Available sinceSept. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKM5
Plasmid#154166PurposeAedes aegypti Rel2 (long isoform) expressed under the IE1 promoter and hr5 enhancer from Autographa californica nuclear polyhedris virus.DepositorInsertRel2 (long isoform)
UseTagsFLAGExpressionInsectMutationPromoterIE1 promoter and hr5 enhancer from Autographa cal…Available sinceAug. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKM28
Plasmid#154165PurposeAedes aegypti Rel1A expressed under the IE1 promoter and hr5 enhancer from Autographa californica nuclear polyhedris virusDepositorInsertRel1A
UseTagsFLAGExpressionInsectMutationPromoterIE1 promoter and hr5 enhancer from Autographa cal…Available sinceAug. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMtb MCM
Plasmid#138296PurposeExpression of Mtb MCM small and large subunitDepositorInsertsmutA
mutB
UseTagsHis tag and TEV cleavage siteExpressionBacterialMutationinserted TEV cleavage site between His-tag and m…PromoterT7Available sinceApril 29, 2020AvailabilityAcademic Institutions and Nonprofits only