Skip to main content
Addgene
Showing: 5661 - 5680 of 151885 results

  1. Sniper2L

    Plasmid
    #193856
    Purpose
    Expresses human codon-optimized Sniper2L and blasticidin resistance: EFS promoter-Sniper2L-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Article
    Kim et al Nat Chem Biol. 2023 Mar 9. doi: 10.1038/
    Insert
    Sniper2L
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Mutation
    E1007L
    Promoter
    EFS
    Available since
    March 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    193856-plasmid-map-sequence-id-382455

    Enlarge

  6. PLJR965

    Plasmid
    #115163
    Purpose
    Sth1 dCas9 TetR and KanR L5 Int attP for M. tuberculosis
    Depositor
    Sarah Fortune
    Article
    Rock et al Nat Microbiol. 2017 Feb 6;2:16274. doi:
    Inserts
    Sth1 sgRNA scaffold
    Sth1 dCas9
    TetR (deleted TetON) MtbCO
    L5 attP
    L5 Int
    KanR
    Use
    Unspecified
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    115163-plasmid-map-sequence-id-222098

    Enlarge

  10. pcDNA3.1-TRPV1exCellHalo

    Plasmid
    #220307
    Purpose
    Mammalian expression vector for a subunit of rat TRPV1 with circularly permutated HaloTag inserted between the S5 and S6 transmembrane helices
    Depositor
    Eric Senning
    Article
    Mott et al J Gen Physiol. 2024 (in press)
    Insert
    rTRPV1exCellHalo (Trpv1 Rat)
    Use
    Tags
    circularly permutated Halotag (Deo et al., Nat Ch…
    Expression
    Mammalian
    Mutation
    linker included to insert cpHaloTag (
    Promoter
    CMV promoter
    Available since
    July 30, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    220307-plasmid-map-sequence-id-436649

    Enlarge

  14. pET29a-human histone 4-in-One

    Plasmid
    #223237
    Purpose
    Polycistronic bacterial coexpression construct for human His-Sumo-H2A, H2B, H3, and Dual-StrepII-H4
    Depositor
    Weixing Zhao
    Inserts
    His6-VSVG-Histone H2A
    Histone H2B
    Histone H3
    Histone H4-TEV-His6-Dual StrepII
    Use
    Tags
    Dual StrepII, His6, TEV protease site, and VSVG
    Expression
    Bacterial
    Mutation
    A103G
    Promoter
    T7
    Available since
    Aug. 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    223237-plasmid-map-sequence-id-443806

    Enlarge

  15. U6-5'+3' MS2-CircRNA

    Plasmid
    #213752
    Purpose
    For circular RNA-mediated prime editor using U6-5'+3' MS2-CircRNA in HEK293T cells
    Depositor
    Caixia Gao
    Article
    Liang et al Nat Biotechnol. 2024 Jan 10. doi: 10.103
    Insert
    5' ribozyme, 5' ligation sequences, 5' MS2, 3' MS2, 3' ligation sequences, 3' ribozyme
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Feb. 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    213752-plasmid-map-sequence-id-422189

    Enlarge

Showing: 5661 - 5680 of 151885 results