-
Plasmid#170125PurposeLentiviral vector carrying the MCP-ADAR2(E488Q) with a nuclear export signalDepositorInsertMCP-ADAR2-DD(E488Q)-NES
UseLentiviralTagsExpressionMammalianMutationPromoterEf1a core promoterAvailable sinceMarch 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
mito NAD+ biosensor
Plasmid#186791PurposeNAD+ biosensor comprised of a circularly permuted Venus fluorescent protein (cpVenus), a bipartite NAD+-binding domain modeled from bacterial DNA ligase, and a localization tag totarget mitochondriaDepositorInsertmito NAD+ biosensor
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(Empty)-PGKBFP2AGFP-W
Plasmid#67979PurposeCas9 activity reporter (control) with BFP and GFP.DepositorInsertU6gRNA cassette, PGKBFP2AGFP cassette, WPRE
UseLentiviralTagsExpressionMutationDeleted BbsI site within WPREPromoterhuman U6 promoter, mouse PGK promoterAvailable sinceSept. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAJ075_hsCRBN
Plasmid#124214PurposeInsect cell expression vector for His6 tagged hsCRBNDepositorInsertCereblon (CRBN Human)
UseTagsHis6-TEVExpressionInsectMutationPromoterAvailable sinceApril 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
LXRE_Luc
Plasmid#177622Purposesynthetic 3X LXRE promoter linked to luciferase in the pTAL vectorDepositorInsert3X LXRE
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceApril 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSG5-HA-p300
Plasmid#89094Purposemammalian expression of full-length human p300 in pSG5DepositorInsertp300 (EP300 Human)
UseTagsHAExpressionMammalianMutationPromoterSV40Available sinceApril 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
PB_iETV2_P2A_GFP_Puro
Plasmid#168805PurposePiggyBac transposon construct with doxycycline inducible ETV2 + GFP expressionDepositorInsertETV2 (ETV2 Human, Synthetic)
UseSynthetic Biology; PiggybacTagsExpressionMammalianMutationPromoterTREAvailable sinceAug. 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti-puro /BRAF-V600E
Plasmid#131724PurposeMammalian Expression of Flag tagged BRAFDepositorInsertBRAF (BRAF Human)
UseLentiviralTags3xFLAGExpressionMammalianMutationV600EPromoterCMVAvailable sinceFeb. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRK5-EGFP-Tau E14
Plasmid#46907Purposeexpresses EGFP tagged Tau E14 in mammalian cellsDepositorInsertTau E14 (MAPT Human)
UseTagsEGFPExpressionMammalianMutationall 14 S/P or T/P amino acid residues (T111, T153…PromoterCMVAvailable sinceJuly 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1-FLAG-SF3B1-WT
Plasmid#82576PurposeExpresses a codon-optimized ORF of human SF3B1 (wild-type)DepositorInserthuman SF3B1-WT (SF3B1 Human)
UseTagsFLAG peptideExpressionMammalianMutationPromoterCMVAvailable sinceSept. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET-pA-Hia5
Plasmid#174372PurposeSEE DEPOSITOR COMMENTS BELOW. Plasmid for IPTG-inducible expression and purification of protein A-Hia5-6xHis (pA-Hia5) for antibody directed DNA (adenine) methylation (as in DiMeLo-seq)DepositorInsertprotein A - hia5
UseTags6xHis and Protein AExpressionBacterialMutationPromoterAvailable sinceSept. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pQCXIN-TetR-mCherry
Plasmid#59417Purposeretroviral construct expressing the Tet repressor (TetR) tagged with mCherryDepositorInsertTet repressor protein (TetR)
UseRetroviralTagsmCherryExpressionMutationPromoterAvailable sinceNov. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
Klotho (membrane)
Plasmid#17712DepositorInsertKlotho (KL Human)
UseTagsV5ExpressionMammalianMutationP101SPromoterAvailable sinceApril 14, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCMV-MmPeg10rc4
Plasmid#174858PurposeExpresses recoded mouse Peg10 in mammalian cellsDepositorInsertrecoded Mus musculus PEG10 (Peg10 Mouse)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceSept. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EFS-SpCas9
Plasmid#104588PurposeAAV vector expressing Myc-tagged SpCas9 under the EFS promoterDepositorInsertStreptococcus pyogenes Cas9
UseAAV and CRISPRTagsMycExpressionMammalianMutationPromoterEFSAvailable sinceDec. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
-
mApple-N1
Plasmid#54567PurposeLocalization: N1 Cloning Vector, Excitation: 568, Emission: 592DepositorTypeEmpty backboneUseTagsmAppleExpressionMammalianMutationPromoterAvailable sinceJuly 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
EGFR L858R
Plasmid#11012PurposeRetroviral construct for expressing human EGFR with L858R mutation in human cellsDepositorInsertEGFR L858R (EGFR Human)
UseRetroviralTagsExpressionMammalianMutationL858R (tumor-derived)PromoterAvailable sinceAug. 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
CCR5-DuET
Plasmid#213201PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertCCR5 (CCR5 Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
A*11:01:01
Plasmid#203281PurposeOverexpress HLA alleleDepositorInsertA*11:01:01 (HLA-A Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJuly 7, 2023AvailabilityAcademic Institutions and Nonprofits only