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pHKMC1: Empty sgRNA for Cloning
Plasmid#67720PurposeEmpty vector for cloning sgRNA. NotI and BamHI for cloning sgRNA. PU6 driven sgRNA.DepositorTypeEmpty backboneUseTagsExpressionWormMutationPromoterPU6Available sinceJuly 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAD-LyseR
Plasmid#99244PurposeFor autolysate production in E. coli BL21-Gold (DE3) strain: constitutively expresses phage lambda endolysin (gene R)DepositorInsertphage lambda gene R (R phage lambda)
UseTagsExpressionBacterialMutationPromoterbeta-lactamase promoterAvailable sinceOct. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pcDNA3-EGFP-Rac1-T17N
Plasmid#12982DepositorInsertRac1 DN (RAC1 Human)
UseTagsEGFPExpressionMammalianMutationT17N Dominant negativePromoterAvailable sinceOct. 27, 2006AvailabilityAcademic Institutions and Nonprofits only -
VPS13A^mCherry
Plasmid#118758PurposeVPS13A internally tagged with mCherry at residue 1372.DepositorInsertVPS13A (VPS13A Human)
UseTagsmCherry inserted at position 1372ExpressionMammalianMutationPromoterAvailable sinceNov. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1-FLAG-Ku70
Plasmid#46957PurposePlasmid for cloning and epression of GFP-FLAG tagged human Ku70 (N-terminal tag). Confers resistance to G418.DepositorInsertKu70 (XRCC6 Human)
UseTagsGFP-FLAGExpressionMammalianMutationPromoterCMVAvailable sinceAug. 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
plenti-UBC-Myc-gate-pGK-HYG
Plasmid#107395PurposeLentiviral gateway destination vectorDepositorTypeEmpty backboneUseLentiviral; Gateway destinationTagsMycExpressionMammalianMutationPromoterAvailable sinceMay 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV Fcgamma RIIa IRES neo
Plasmid#14948DepositorInsertFcgamma RIIa (FCGR2A Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 21, 2007AvailabilityAcademic Institutions and Nonprofits only -
pEFIRES-P-mCherry-HPos
Plasmid#87159PurposeFluorescent marker of lipid droplets (LDs)DepositorInsertHPos
UseTagsmCherryExpressionMammalianMutationPromoterEF-1-alphaAvailable sinceMay 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pYPQ153
Plasmid#69304PurposeGateway entry vector with pco-dCas9-3X(SRDX)DepositorInsertpco-dCas9-3X(SRDX) (Plant codon-optimized)
UseCRISPRTags2XFLAGExpressionMutationSRDX (X3) repressor fusion; NLS fusionPromoterAvailable sinceOct. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-DU422gp160
Plasmid#100926PurposeMammalian expression plasmid for Env from the DU422.1 HIV-1 isolateDepositorInsertHIV-1 (DU422.1) Env
UseTagsCD5 leader sequenceExpressionMammalianMutationCodon-optimized synthetic genePromoterCMVAvailable sinceMarch 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-B41gp160
Plasmid#123247PurposeMammalian expression plasmid for Env from the B41 HIV-1 isolateDepositorInsertHIV-1 (B41) Env
UseTagsCD5 leader peptideExpressionMammalianMutationCodon-optimized synthetic genePromoterCMVAvailable sinceApril 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFLAG-ZYG11A
Plasmid#110551Purposeexpresses a FLAG-tagged human ZYG11A protein in mamalian cellsDepositorInsertZYG11A (ZYG11A Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceMay 31, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET-28c(+)-YTHDF2
Plasmid#102275PurposeFor bacterial expression of His-tagged human YTHDF2DepositorInsertYTHDF2 (YTHDF2 Human)
UseTags6X HISExpressionBacterialMutationPromoterT7Available sinceSept. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA mouse NHERF-1 (1-355)
Plasmid#32705DepositorInsertsolute carrier family 9 (sodium/hydrogen exchanger), member 3 regulator 1 (Nherf1 Mouse)
UseTagsnoneExpressionMammalianMutationnonePromotercmvAvailable sinceFeb. 14, 2012AvailabilityAcademic Institutions and Nonprofits only -
pcDNA_DENV2-NS2B3_V5
Plasmid#115906PurposeMammalian expression of DENV2 NS2B3 protein with a C-terminal V5 tagDepositorInsertDENV2 NS2B3 protease
UseTagsV5 tagExpressionMammalianMutationPromoterCMVAvailable sinceOct. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBS-KS-attB2-SA(0)-T2A-Gal4
Plasmid#62899PurposeCreating Gal4 drivers from MiMIC lines with inserts into coding introns in Phase 0DepositorInsertT2A-Gal4
UseTagsExpressionMutationPromoterAvailable sinceMay 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
CMV-PKCb1-mEGFP-N1
Plasmid#112265PurposeITRACKbeta/IDOCKSbeta Donor constructDepositorInsertprotein kinase c beta (Prkcb Mouse)
UseTagsmEGFPExpressionMammalianMutationPromoterCMVAvailable sinceOct. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS/ES-EnvA-VSVg-WPRE-pA
Plasmid#87221Purposeto package wild type EnvA psudotyped lentivirusDepositorInsertEnvA-VSVg
UseMouse Targeting and Synthetic BiologyTagsExpressionMammalianMutationPromoterCAGAvailable sinceMarch 14, 2017AvailabilityAcademic Institutions and Nonprofits only
