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Plasmid#160733PurposeMini-transposon donor plasmid for V. cholerae CAST system. Mini-transposon has MmeI site in R end for Tn-seq. pUC19 vector backbone.DepositorInsertVchCAST donor DNA
UseTagsExpressionBacterialMutationPromoterN/AAvailable sinceOct. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTP341 pHAGE2 CMV TagBFP-3xFLAG
Plasmid#199391PurposeLentiviral transfer plasmid for constitutive expression of a TagBFP-3xFLAG in mammalian cellsDepositorInsertTagBFP-3xFLAG
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTP924 pHAGE2 CMV GFP
Plasmid#199392PurposeLentiviral transfer plasmid for constitutive expression of a GFP in mammalian cellsDepositorInsertGFP
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
HBT_pcoCASphi_version2
Plasmid#197932PurposeCloning vector with Arabidopsis codon optimized Casphi-2 protein, with SV40 NLS at the C-terminalDepositorInsertpcoCasphi-2
UseCRISPRTags2xNLS-2xFLAGExpressionPlantMutationPromoter35SPPDK promoterAvailable sinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSL2622 (pCas7_Vch)
Plasmid#200894PurposeExpresses human codon-optimized V. cholerae Cas7 from a CMV promoter.DepositorInsertVchCas7
UseTagsNLSExpressionMammalianMutationPromoterAvailable sinceJune 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAtetO_6xHis-Klentaq
Plasmid#157862PurposePlasmid for the aTc-inducible expression of the KlentaqDepositorInsertKlentaq
UseTags6xHisExpressionBacterialMutationPromotertet PAAvailable sinceAug. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
HP138-neo
Plasmid#134247PurposeCas9 in a transposon array under a Tet inducible promoter. For making inducible Cas9 cell lines. Neo/G418 selectionDepositorInsertContains Cas9
UseCRISPRTagsExpressionMutationPromoterAvailable sinceFeb. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTS103 pHAGE2 CMVtetO2 MCS-22xGS-GFP
Plasmid#199356PurposeLentiviral transfer plasmid for doxycycline inducible expression of a C-terminally GFP-tagged protein in mammalian cells.DepositorTypeEmpty backboneUseLentiviralTagsEGFPExpressionMammalianMutationWTPromoterAvailable sinceApril 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3/CyclinB1-GFP (28)
Plasmid#39871DepositorInsertCyclinB1 (CCNB1 Human)
UseTagsGFPExpressionMammalianMutationV5APromoterAvailable sinceJune 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pDEST-ORF-V2
Plasmid#73638PurposeGatewayâ„¢ destination vector encoding C-terminal fusion with Venus fluorescent protein fragment 2 (V2).DepositorTypeEmpty backboneUseTagsVenus fragment 2 (V2)ExpressionMammalianMutationPromoterCMVAvailable sinceJune 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
FLAG-MRGBP
Plasmid#15325DepositorInsertMRG-binding protein (MRGBP Human)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceAug. 17, 2007AvailabilityAcademic Institutions and Nonprofits only -
Lenti-EF1alpha-dCas9-10xGCN4_Hygro
Plasmid#192651Purpose3rd generation lenti vector encoding dCas9-10xGCN4 (Suntag) with 2A Hygro resistance markerDepositorInsertdCas9-10xGCN4
UseCRISPR and LentiviralTagsExpressionMammalianMutationD10A and N863A in Cas9PromoterEF1aAvailable sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
rASAP
Plasmid#178205PurposeRatiometric version of the genetically encoded voltage indicator ASAP2s-I67T-Q397R.DepositorInsertrASAP
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSmart-EF1a-Nla-cas3
Plasmid#178881PurposeExpression of human codon-optimized NlaCas3 with C terminal NLS and HA tagDepositorInsertEF1a-NlaCas3c-bGH polyA
UseTagsHA and NLSExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
Mito-ABKAR
Plasmid#61509PurposeExpression of AMPK biosensor targeted to the mitochondriaDepositorInsertAMPK Biosensor
UseTagsMitochondrial targeting signal (DAKAP1-30)ExpressionMammalianMutationPromoterCMVAvailable sinceApril 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
Cyto-ABKAR
Plasmid#61510PurposeExpression of AMPK biosensorDepositorInsertAMPK Biosensor
UseTagsNESExpressionMammalianMutationPromoterCMVAvailable sinceApril 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Tag3B-CCre
Plasmid#106369PurposeC-terminal part of the split-Cre SystemDepositorInsertCCre
UseCre/LoxTagsc-mycExpressionMammalianMutationPromoterCMVAvailable sinceMarch 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDEST40-MFRN2
Plasmid#50546PurposeExpression of MFRN2 gene in mammalian cellsDepositorInsertMrfn2 (SLC25A28 Human)
UseTagsV5ExpressionMammalianMutationPromoterAvailable sinceJan. 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
SPI1_pet28a
Plasmid#131653PurposeBacterial expression of ETS gene SPI1DepositorInsertSPI1 (SPI1 Human)
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceOct. 4, 2019AvailabilityAcademic Institutions and Nonprofits only