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pMSCV-human-pro-IL-18_V47N_I48N

Plasmid

#214319

Purpose

Expression of gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

IL18 (IL18 Human)

Use

Retroviral

Tags

Flag and Myc

Expression

Mutation

V47N_I48N

Promoter

MESV

Available since

Feb. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pMSCV-human-pro-IL-18_LES33AAA

Plasmid

#214321

Purpose

Expression of gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

IL18 (IL18 Human)

Use

Retroviral

Tags

Flag and Myc

Expression

Mutation

LES33AAA

Promoter

MESV

Available since

Feb. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pMSCV-human-pro-IL-18_DDEN29ENGD

Plasmid

#214320

Purpose

Expression of gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

IL18 (IL18 Human)

Use

Retroviral

Tags

Flag and Myc

Expression

Mutation

DDEN29ENGD

Promoter

MESV

Available since

Feb. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pMSCV-human-pro-IL-18_E192K_D193K

Plasmid

#214318

Purpose

Expression of gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

IL18 (IL18 Human)

Use

Retroviral

Tags

Flag and Myc

Expression

Mutation

E192K_D193K

Promoter

MESV

Available since

Feb. 14, 2024

Availability

Academic Institutions and Nonprofits only

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WT_IFL_G1A-T2A_R

Plasmid

#200183

Purpose

WT PA14 I-F Leader and G1A-T2A Repeat

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

Insert

Wildtype I-F Leader and a mutant I-F Locus (G1A-T2A)

Use

Tags

Expression

Bacterial

Mutation

G1A-T2A in the I-F repeat

Promoter

Available since

Dec. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pFSynW pHluorin-SYT9 IRES mRuby3

Plasmid

#195698

Purpose

Lentiviral plasmid encoding SYT9 with an N-terminal pHluorin tag followed by an internal ribosomal entry site followed by mRuby3 under the human synapsin promoter

Depositor

Edwin Chapman

Article

Seibert et al J Neurosci. 2023 Mar 1;43(9):1475-1491.

Insert

Syt9 (Syt5 Rat)

Use

Lentiviral

Tags

pHluorin

Expression

Mutation

Promoter

Available since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pFSynW Syt9 IRES mRuby3

Plasmid

#195700

Purpose

Lentiviral plasmid encoding full-length mouse SYT9 followed by an internal ribosomal entry site followed by mRuby under the human synapsin promoter

Depositor

Edwin Chapman

Article

Seibert et al J Neurosci. 2023 Mar 1;43(9):1475-1491.

Insert

Syt9 (Syt5 Mouse)

Use

Lentiviral

Tags

Expression

Mutation

encodes only the first 68 amino acids of Tac1

Promoter

Available since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX SYT9 C2B D330, 332N

Plasmid

#195705

Purpose

Bacterial expression plasmid encoding GST-tagged SYT9 C2B domain with D330, 332N mutations

Depositor

Edwin Chapman

Article

Seibert et al J Neurosci. 2023 Mar 1;43(9):1475-1491.

Insert

Syt9 (Syt5 Mouse)

Use

Tags

GST

Expression

Bacterial

Mutation

encodes amino acids 253-344 of mouse SYT9 with D3…

Promoter

Available since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX SYT9 C2AB D197,199,330,332N

Plasmid

#195706

Purpose

Bacterial expression plasmid encoding GST-tagged SYT9 C2AB domain with D197, 199, 330, 332N mutations

Depositor

Edwin Chapman

Article

Seibert et al J Neurosci. 2023 Mar 1;43(9):1475-1491.

Insert

Syt9 (Syt5 Mouse)

Use

Tags

GST

Expression

Bacterial

Mutation

encodes amino acids 104-386 of mouse SYT9 with D1…

Promoter

Available since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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PET-HypaSpCas9-NLS-6xHis

Plasmid

#207384

Purpose

Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

HypaSpCas9

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Mutation

N692A, M694A, Q695A, H698A

Promoter

T7

Available since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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PET-evoSpCas9-NLS-6xHis

Plasmid

#207389

Purpose

Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

evoSpCas9

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Mutation

M495V, Y515N, K526E, R661Q

Promoter

T7

Available since

Sept. 26, 2023

Availability

Academic Institutions and Nonprofits only

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PET-eSpCas9-NLS-6xHis

Plasmid

#207379

Purpose

Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

eSpCas9

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Mutation

K848A, K1003A, R1060A

Promoter

T7

Available since

Sept. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pYG215

Plasmid

#200837

Purpose

Transcribes a glmZ' (1-146)-gfp fusion. gfp can be replaced via AgeI/XbaI-sites with gene of interest to release its RNA with a 5' monophosphate upon cleavage.

Depositor

Boris Görke

Article

Schilder et al Nucleic Acids Res. 2023 Mar 29:gkad226.

Insert

glmZ (glmZ Escherichia coli str. K-12 substr. MG1655)

Use

Synthetic Biology

Tags

GFP

Expression

Bacterial

Mutation

Promoter

P-LlacO-1

Available since

Aug. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pEXPA10-NLS-3xmCitrine

Plasmid

#192523

Purpose

Fluorescent reporter for plant responses to bacterial infection

Depositor

Gitta Coaker

Article

Zhu et al Cell Rep. 2023 Jun 20;42(7):112676. doi:

Insert

EXPANSIN A10 (EXPA10 Mustard Weed)

Use

Tags

3xmCitrine and NLS

Expression

Plant

Mutation

Promoter

EXPA10

Available since

June 23, 2023

Availability

Academic Institutions and Nonprofits only

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pPIP1;4-NLS-3xmCitrine

Plasmid

#192524

Purpose

Fluorescent reporter for plant responses to bacterial infection

Depositor

Gitta Coaker

Article

Zhu et al Cell Rep. 2023 Jun 20;42(7):112676. doi:

Insert

PLASMA MEMBRANE INTRINSIC PROTEIN 1;4 (PIP1;4 Mustard Weed)

Use

Tags

3xmCitrine and NLS

Expression

Plant

Mutation

Promoter

PIP1;4

Available since

June 23, 2023

Availability

Academic Institutions and Nonprofits only

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pBactin-mOrange2-CI

Plasmid

#196852

Purpose

Expression of red fluorescent reporter mOrange driven by the strong Beta-actin promoter

Depositor

Frank Bradke

Article

Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

Insert

mOrange2

Use

Tags

Expression

Mammalian

Mutation

None (wt)

Promoter

Chicken bactin (plus Chicken bactin intron)

Available since

May 16, 2023

Availability

Academic Institutions and Nonprofits only

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AV13_pCAG.Cas9.gRNA.NT

Plasmid

#199260

Purpose

Expression construct encoding SpCas9 nuclease and a control non-targeting guide RNA

Depositor

Manuel Goncalves

Article

Wang et al Nucleic Acids Res. 2021 Jan 25;49(2):117

Inserts

SpCas9 nuclease
Non-targeting (NT) control gRNA

Use

CRISPR

Tags

SV40 NLS

Expression

Mammalian

Mutation

Promoter

CAG promoter and RNA polymerase III promoter for …

Available since

April 27, 2023

Availability

Academic Institutions and Nonprofits only

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pET-20b_HtrA2(delta(133))_S306A_6His

Plasmid

#197975

Purpose

E. coli expression of HtrA2(delta133)-6His

Depositor

Eric Fischer

Article

Hunkeler et al Science. 2023 Mar 17;379(6637):1105-1111

Insert

HtrA2 (HTRA2 Human)

Use

Tags

6His

Expression

Bacterial

Mutation

delta(1-133), S306A

Promoter

Available since

April 19, 2023

Availability

Academic Institutions and Nonprofits only

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pDARMO_CMVT_3xFLAG_BIRC6_delta(Helix)

Plasmid

#197969

Purpose

Mammalian cell expression of 3xFLAG-BIRC6_delta(Helix)

Depositor

Eric Fischer

Article

Hunkeler et al Science. 2023 Mar 17;379(6637):1105-1111

Insert

BIRC6 (BIRC6 Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

L1332V, delta(1616-1666)

Promoter

Available since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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pDARMO_CMVT_3xFLAG_BIRC6_delta(Helix)_BIRmut

Plasmid

#197970

Purpose

Mammalian cell expression of 3xFLAG-BIRC6_delta(Helix)/BIRmut

Depositor

Eric Fischer

Article

Hunkeler et al Science. 2023 Mar 17;379(6637):1105-1111

Insert

BIRC6 (BIRC6 Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

C328S, C331S, L1332V, delta(1616-1666)

Promoter

Available since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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