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pET30a-SRAP-delCys2

Plasmid

#84568

Purpose

Encodes murine SRAP domain (250aa) of Hmces/Srap1 with deletion of catalytic Cys2

Depositor

Douglas Feldman

Article

Kweon et al Cell Rep. 2017 Oct 10;21(2):482-494. doi

Insert

Srap1 (Hmces Mouse)

Use

Tags

6xHis and S-Tag

Expression

Bacterial

Mutation

deletion of Cys2

Promoter

T7

Available since

Nov. 18, 2016

Availability

Academic Institutions and Nonprofits only

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pBS/U6/RNAi gamma-tubulin shRNA mouse

Plasmid

#70666

Purpose

Reduced the expression of gamma-tubulin in murine cells

Depositor

Maria Alvarado-Kristensson

Article

Hoog et al FASEB J. 2011 Nov;25(11):3815-27. doi: 1

Insert

5´-GGCTCATGATGGCCAACCACCAAGCTTGGTGGTTGGCCATCATGAGCCCTTTTTC-3´ 5´-CCGAGTACTACCGGTTGGTGGTTCGAACCACCAACCGGTAGTACTCGGGAAAAAGTTAA-3´ (Tubg1, Tubg2 Mouse)

Use

U6 promoter

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Dec. 17, 2015

Availability

Academic Institutions and Nonprofits only

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PD-L1-miSFIT-4x

Plasmid

#124684

Purpose

Tuning expression of PD-1

Depositor

Tudor Fulga

Article

Michaels et al Nat Commun. 2019 Feb 18;10(1):818. doi:

Insert

PD-L1 (CD274 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

minimal CMV

Available since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3 PARL-FLAG-CT S65D+T69D+S70D

Plasmid

#13617

Depositor

Luca Pellegrini

Article

Jeyaraju et al Proc Natl Acad Sci U S A. 2006 Dec 5. 10

Insert

Presenilin-associated rhomboid-like (PARL, PARL Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

changed Serine 65, Thr 69 and Ser70 to Asp

Promoter

Available since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only
pGP-CMV-GCaMP3-44

Plasmid

#91780

Purpose

Mammalian expression of low affinity calcium-sensing fluorescent protein

Depositor

Douglas Kim, GENIE Project

Article

de Juan-Sanz et al Neuron. 2017 Jan 30. pii: S0896-6273(17)

Insert

GCaMP3-44

Use

Tags

6xHis, T7 epitope, and Xpress tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 27, 2017

Availability

Academic Institutions and Nonprofits only

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pSBK.009

Plasmid

#187209

Purpose

Expresses retron Eco1 ncRNA v35 (long a1/a2, barcode 1 [CCT-AGG]), and Cas1 + Cas2

Depositor

Seth Shipman

Article

Bhattarai-Kline et al Nature. 2022 Aug;608(7921):217-225. doi:

Inserts

Retron Eco1 ncRNA v35 (long a1/a2, barcode 1 [CCT-AGG])
Cas1 + Cas2

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

T7/Lac

Available since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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6599-SUMO1GG

Plasmid

#13377

Depositor

Anne Dejean

Article

Seeler et al Mol Cell Biol. 2001 May . 21(10):3314-24

Insert

SUMO1 (SUMO1 Human)

Use

Tags

Expression

Mammalian

Mutation

mature form ending with GG

Promoter

Available since

Dec. 8, 2006

Availability

Academic Institutions and Nonprofits only

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pg-dsRedXP-HIF-2alpha

Plasmid

#87209

Purpose

N-terminally dsRedXP-tagged human HIF-2a/EPAS

Depositor

Violaine Sée

Article

Taylor et al Open Biol. 2016 Sep;6(9). pii: 160195.

Insert

HIF-2a (EPAS1 Human)

Use

Tags

dsRedXP

Expression

Mammalian

Mutation

Q868E

Promoter

CMV

Available since

Sept. 4, 2018

Availability

Academic Institutions and Nonprofits only

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RGG-mCherry-RGG-eDHFR

Plasmid

#164648

Purpose

Two RGG domains tagged with mCherry and eDHFR

Depositor

Huaiying Zhang, Michael Lampson

Article

Zhang et al Mol Biol Cell. 2020 Aug 15;31(18):2048-2

Insert

RGG

Use

Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

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PKM-IRES-hrGFP

Plasmid

#73405

Purpose

Mammalian expression of mouse PKM and hrGFP

Depositor

Raymond Swanson

Article

Brennan-Minnella et al Cell Death Dis. 2013 Apr 4;4:e580. doi:

Insert

PKM (Prkcz Mouse)

Use

Tags

3xFLAG and pIRES-hrGFP-2a

Expression

Mammalian

Mutation

truncated, constitutively active form

Promoter

CMV

Available since

Feb. 19, 2016

Availability

Academic Institutions and Nonprofits only
QQR1 variant

Plasmid

#138564

Purpose

Expresses human codon-optimized SpCas9-QQR1 and blasticidin resistance: EFS promoter-QQR1-NLS-FLAG-P2A-BSD

Depositor

Hyongbum Kim

Article

Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

Insert

QQR1

Use

CRISPR and Lentiviral

Tags

BSD, FLAG, and NLS

Expression

Mammalian

Mutation

G1218R, N1286Q, I1331F, D1332K, R1333Q, R1335Q, T…

Promoter

EFS

Available since

June 9, 2020

Availability

Academic Institutions and Nonprofits only

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1352wUV2 F3bbs kan NNNTGGGCGk

Plasmid

#18756

Depositor

Scot Wolfe

Article

Meng et al Nat Biotechnol. 2008 Jun . 26(6):695-701

Insert

3 tandem zinc fingers

Use

Tags

Expression

Bacterial

Mutation

A Kan cassette (1090bp) is inserted between two b…

Promoter

Available since

July 11, 2008

Availability

Academic Institutions and Nonprofits only

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pDsRed2-C1-Ahi1

Plasmid

#30495

Depositor

Joseph Gleeson

Article

Lancaster et al Nat Med. 2009 Sep . 15(9):1046-54.

Insert

Ahi1 (AHI1 Human)

Use

Tags

DsRed2

Expression

Mammalian

Mutation

Promoter

Available since

June 6, 2011

Availability

Academic Institutions and Nonprofits only
1352wUV2 F1bbs kan GCGTGGNNN

Plasmid

#18753

Depositor

Scot Wolfe

Article

Meng et al Nat Biotechnol. 2008 Jun . 26(6):695-701

Insert

3 tandem zinc fingers

Use

Tags

Expression

Bacterial

Mutation

A Kan cassette (1090bp) is inserted between two b…

Promoter

Available since

July 11, 2008

Availability

Academic Institutions and Nonprofits only

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OVA-miSFIT-2xPerfect

Plasmid

#124673

Purpose

Tuning expression of cytosolic Ovalbumin coupled to EGFP

Depositor

Tudor Fulga

Article

Michaels et al Nat Commun. 2019 Feb 18;10(1):818. doi:

Insert

Cyto-Ovalbumin

Use

Tags

Expression

Mammalian

Mutation

Truncated OVA for cytoplasmic expression

Promoter

minimal CMV

Available since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-C-intein-C-spC9-H840A-N863A-2xNLS-hGH

Plasmid

#112211

Purpose

Targeted DNA methylation

Depositor

Xingxu Huang

Article

Lu et al Cell Death Dis. 2020 Feb 3;11(2):85. doi

Insert

C-terminus of dCas9

Use

AAV

Tags

Expression

Mutation

D10A

Promoter

Available since

Oct. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pEGFP-Map7-Start-1351

Plasmid

#46075

Depositor

Edgar Gomes

Article

Metzger et al Nature. 2012 Mar 18;484(7392):120-4. doi

Insert

Map7-Start-1351 (Map7 Mouse)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

July 12, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3 PARL-FLAG-CT S65A+T69A+S70A

Plasmid

#13616

Depositor

Luca Pellegrini

Article

Jeyaraju et al Proc Natl Acad Sci U S A. 2006 Dec 5. 10

Insert

Presenilin-associated rhomboid-like (PARL, PARL Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

changed Ser 65, Thr 69 and Ser 70 to Alanine

Promoter

Available since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only
pMal-SLFN2

Plasmid

#174322

Purpose

Bacterial expression of MBP-tagged mouse SLFN2

Depositor

Bruce Beutler

Article

Yue et al Science. 2021 May 14;372(6543). pii: 372

Insert

SLFN2 (Slfn2 Mouse)

Use

Tags

MBP

Expression

Bacterial

Mutation

Promoter

tac promoter

Available since

Oct. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pSC-NF8-d1-eGFP

Plasmid

#122036

Purpose

AAV vector, NFkappaB-dependent eGFP expression

Depositor

Liliane Tenenbaum

Article

Chtarto et al PLoS One. 2013;8(1):e53156. doi: 10.1371

Insert

eGFP

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

April 17, 2019

Availability

Academic Institutions and Nonprofits only

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