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Plasmid#195426PurposeTransient, constitutive expression of synZiFTR: ZF3-p65DepositorInsertpUb - 3XFLAG - NLS - ZF3-5 (ZF3) - p65 - RbGpA
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMZ-185
Plasmid#195433PurposeTransient, constitutive expression of synZiFTR: ZF10-p65DepositorInsertpUb - 3XFLAG - NLS - ZF10-1 (ZF10) - p65 - RbGpA
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSUPER IKKe
Plasmid#26211DepositorInsertpSUPER IKK epsilon (IKBKE Human)
UseRNAiTagsExpressionMutationPromoterAvailable sinceAug. 24, 2011AvailabilityAcademic Institutions and Nonprofits only -
TBP6.7 non-targeting gRNA lambda phage
Plasmid#132547Purposeexpresses gRNA for TBP-based CIRTSDepositorInsertgRNA TBP-based CIRTS
UseTagsExpressionMammalianMutationPromoterhU6 promoterAvailable sinceSept. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCK364.306
Plasmid#192642PurposeExpresses dSpRY and MCP-SoxS(R93A/S101A) and scRNA J306 on p15A-CmRDepositorInsertJ306 scRNA
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterBBa_J23119Available sinceJan. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCFD3-frame_selector_0
Plasmid#127553PurposeDrosophila expression of frame selector sgRNA 0 that targets CRISPaint site for homology-independent knock-inDepositorInsertsgRNA frame 0
UseCRISPRTagsExpressionInsectMutationPromoterdU6:3Available sinceJuly 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO/FLAG-HA-SBP-TNRC6A
Plasmid#42044DepositorInsertTNRC6A (TNRC6A Human)
UseTagsFLAG, HA, and SBPExpressionMammalianMutationPromoterCMVAvailable sinceMarch 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCFD3-frame_selector_1
Plasmid#127554PurposeDrosophila expression of frame selector sgRNA 1 that targets CRISPaint site for homology-independent knock-inDepositorInsertsgRNA frame 1
UseCRISPRTagsExpressionInsectMutationPromoterdU6:3Available sinceJuly 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCFD3-frame_selector_2
Plasmid#127555PurposeDrosophila expression of frame selector sgRNA 2 that targets CRISPaint site for homology-independent knock-inDepositorInsertsgRNA frame 2
UseCRISPRTagsExpressionInsectMutationPromoterdU6:3Available sinceJuly 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPRv2-TRAF6-targeting sgRNA 2
Plasmid#131346PurposegRNA targeting mouse TRAF6DepositorInsertgRNA targeting mouse TRAF6 (Traf6 Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pXPR_003 sgMSLN
Plasmid#193588PurposeMSLN knockoutDepositorInsertsgMSLN (MSLN Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-K-I-N-CAAX
Plasmid#41640PurposeExpresses caged Cdc42 GEF intersectin: Intersectin Dbl homology (DH) domain fused to wild-type Dronpa145K at C terminus and Dronpa145N at N terminus, for optogenetic activationDepositorInsertDronpa145K-IntersectinDH-Dronpa145N-CAAX
UseTagsCAAX boxExpressionMammalianMutationK145N on DronpaPromoterCMVAvailable sinceDec. 21, 2012AvailabilityAcademic Institutions and Nonprofits only -
pADH143
Plasmid#90989PurposeNAT-marked "empty" gRNA expression construct; part 2 of 2 of C.mal LEUpOUT CRISPR system. Use with pADH140 CAS9 expression construct.DepositorInsertNAT 2 of 2, pSNR52, empty gRNA, gRNA conserved, C. mal LEU2 2 of 2
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJune 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
2X_pX458_pSpCas9(BB)-2A-GFP_SOX17_bKO
Plasmid#172225PurposeCas9-2A-GFP expression vector bearing two sgRNAs targeting the centromeric human SOX17 CTCF-boundary.DepositorInsertsgRNA
UseCRISPRTags3XFLAG and GFPExpressionMammalianMutationPromoterCbhAvailable sinceSept. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMZ-176
Plasmid#195424PurposeTransient, constitutive expression of synZiFTR: ZF1-p65DepositorInsertpUb - 3XFLAG - NLS - ZF1-3 (ZF1) - p65 - RbGpA
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pZac2.1-GfaABC1D -IgK-TurboID-HA
Plasmid#219810PurposeSecreted HA-tagged TurboID for extracellular proximity labelingDepositorInsertIgK (Igk Mouse)
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceJuly 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-N-I-N-CAAX
Plasmid#41686PurposeExpresses caged Cdc42 GEF intersectin: Intersectin Dbl homology (DH) domain fused to Dronpa145N at C terminus and at N terminus, for optogenetic activationDepositorInsertDronpa145N-IntersectinDH-Dronpa145N-CAAX
UseTagsCAAX boxExpressionMammalianMutationK145N on DronpaPromoterCMVAvailable sinceDec. 21, 2012AvailabilityAcademic Institutions and Nonprofits only -
pegRNA_LMNA_CR-2-5
Plasmid#199275PurposepegRNA used to correct LMNA (c.1824C > T) mutationDepositorInsertLMNA 1824TtoC pegRNA
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCfB9336 (pgRNA_II-1_NatMX)
Plasmid#161589PurposeEasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site II-1DepositorInsertguiding RNA
UseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceAug. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR_eGFP_191
Plasmid#111821PurposeKnockout eGFPDepositorInsertgRNA targeting eGFP 171-191
UseCRISPRTagsmCherryExpressionBacterial and MammalianMutationPromoterU6Available sinceAug. 2, 2018AvailabilityAcademic Institutions and Nonprofits only