Optical control of protein activity by fluorescent protein domains.
Zhou XX, Chung HK, Lam AJ, Lin MZ
Science. 2012 Nov 9;338(6108):810-4. doi: 10.1126/science.1226854.
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Article
Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
41640 | pcDNA3-K-I-N-CAAX | Expresses caged Cdc42 GEF intersectin: Intersectin Dbl homology (DH) domain fused to wild-type Dronpa145K at C terminus and Dronpa145N at N terminus, for optogenetic activation |
41642 | pcDNA3-K-I-CAAX | Catalytically active control, intersectin Dbl homology (DH) domain fused to wild-type Dronpa |
41643 | pcDNA3-I-K-CAAX | Catalytically active control, intersectin Dbl homology (DH) domain fused to wild-type Dronpa |
41644 | pcDNA3-K-CAAX | Expresses wild-type Dronpa fused to the membrane-anchoring K-Ras C-terminal farnesylation motif (CAAX box) |
41645 | pcDNA3-mNeptune2-N | Expresses far-red FP mNeptune2 fused to Dronpa145N. Dronpa145N oligomerizes under violet light (390-400nm) and dissociates under cyan light (490-500nm) |
41686 | pcDNA3-N-I-N-CAAX | Expresses caged Cdc42 GEF intersectin: Intersectin Dbl homology (DH) domain fused to Dronpa145N at C terminus and at N terminus, for optogenetic activation |