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Plasmid#118027PurposeAAV vector for sparse and bright labeling in a cell-type specific mannerDepositorInsertTRE-DIO-FLPo
UseAAVTagsExpressionMutationPromoterTREAvailable sinceFeb. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKH151_PB_flag-LC3B_wt_IRES-BFP
Plasmid#214976PurposeFlag tagged LC3BDepositorInsertFLAG-LC3B (MAP1LC3B Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
SPLICS Po-ER Short P2A
Plasmid#164114PurposeDetect the long Peroxisomes-Endoplasmic reticulum contactDepositorInsertSplit YFP Po-ER Short P2A
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
EF1a_MYCN_P2A_Hygro_Barcode
Plasmid#120463PurposeBarcoded lentiviral vector to express MYCN in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.DepositorInsertMYCN (MYCN Human)
UseLentiviralTagsExpressionMutationPromoterEF1aAvailable sinceFeb. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
GFP-noPS
Plasmid#177943PurposeTransient mammalian expression of eGFP. Lacks packaging sequence and is used as a negative control transfer plasmid for SARS-CoV-2 virus-like particles.DepositorInserteGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
LLP775_αGCN4-p65HSF1-CO
Plasmid#211768PurposeSunTag counterpart binding domain, aGCN4, fused to transcriptional activator p65HSF1, with GFP selectionDepositorInsertaGCN4-p65HSF1
UseTags3xTy1ExpressionMammalianMutationLast 300 bp codon-optimised (CO) to detect p65HSF…PromoterpEF1a and pSV40Available sinceDec. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
LLP778_Traptavidin-p65HSF1-CO
Plasmid#211771PurposeAviTag counterpart binding domain, Traptavidin, fused to transcriptional activator p65HSF1, with sfGFP selectionDepositorInsertTraptavidin-p65HSF1
UseTags3xV5ExpressionMammalianMutationLast 300 bp codon-optimised (CO) to detect p65HSF…PromoterpEF1a and pSV40Available sinceDec. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
LLP776_SpyCatcher-p65HSF1-CO
Plasmid#211769PurposeSpyTag counterpart binding domain, SpyCatcher, fused to transcriptional activator p65HSF1, with GFP selectionDepositorInsertSpyCatcher-p65HSF1
UseTags3xFLAGExpressionMammalianMutationLast 300 bp codon-optimised (CO) to detect p65HSF…PromoterpEF1a and pSV40Available sinceDec. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGGAselect
Plasmid#195714PurposepGGAselect is a cloning vector for Golden Gate Assembly using BsaI, BsmBI, and BbsI Type IIS restriction enzymes.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
CMV-T7-hOsa2
Plasmid#17987DepositorInsertOsa2 (ARID1B Human)
UseTagsT7 epitopeExpressionMammalianMutationPromoterAvailable sinceAug. 19, 2008AvailabilityAcademic Institutions and Nonprofits only -
4X PRE TK luc
Plasmid#206159PurposeLuciferase reporter construct containing 4 consensus PGR binding sites upstream of a minimal TK promoter, derived from Addgene #11350DepositorInsert4X PRE TK luciferase
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
SPLICS Po-ER Long P2A
Plasmid#164113PurposeDetect the long Peroxisomes-Endoplasmic reticulum contactDepositorInsertSplit YFP Po-ER Long P2A
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
EF1a_SOX2_P2A_Hygro_Barcode
Plasmid#120480PurposeBarcoded lentiviral vector to express SOX2 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.DepositorInsertSOX2 (SOX2 Human)
UseLentiviralTagsExpressionMutationPromoterEF1aAvailable sinceFeb. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28a-DONSON
Plasmid#208797PurposeXenopus laevis His6-Myc-DONSON wild typeDepositorInsertDONSON (donson.L Frog)
UseTagsHis and MycExpressionBacterialMutationPromoterT7Available sinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCSD_2xNLS-SpCas9WT-NLS-3xHA-NLS-SaCas9WT-2xNLS
Plasmid#107320PurposeExpresses SpCas9 fused to SaCas9 in mammalian cellsDepositorInsertSpCas9-SaCas9 fusion
UseCRISPRTagsNLSExpressionMammalianMutationSpCas9 is fused to SaCas9PromoterCMV IE94Available sinceNov. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLYS5-MCU-Flag
Plasmid#50054PurposeExpression of human MCU in mammalian cellsDepositorInsertMCU-Flag (MCU Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceJan. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-TRC.mKO2
Plasmid#85208PurposeBackbone for lentiviral gene silencing with monomeric Kusabira-Orange2 expressionDepositorTypeEmpty backboneUseLentiviral and RNAiTagsExpressionMammalianMutationPromoterRNA polymerase III promoter for human U6 snRNA fo…Available sinceOct. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHR-CMV-TetO2_mTurquoise2
Plasmid#113895PurposeMammalian (inducible) expression of cytosolic mTurquoise2DepositorInsertmonomeric Turquoise2
UseLentiviralTagsExpressionMutationPromoterCMV-MIE-TetO2Available sinceAug. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEF5B-FRT-GFP-αTAT1[D157N]
Plasmid#27100DepositorInsertαTubulin K40 acetyltransferase (ATAT1 Human)
UseTagsGFP and S-tagExpressionMammalianMutationD157NPromoterAvailable sinceJan. 5, 2011AvailabilityAcademic Institutions and Nonprofits only -
pDN0606 (pDEST-DHFR F[3] N-term,HygR)
Plasmid#210488PurposepDEST vector to tag gene of interest with DHFR F[3] on the N-terminus to perform DHFR-PCA in S. cerevisiae.DepositorTypeEmpty backboneUseSynthetic Biology; Destination vector for gateway…TagsExpressionYeastMutationPromoterADH1Available sinceDec. 22, 2023AvailabilityAcademic Institutions and Nonprofits only