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Plasmid#184131PurposeFor rescuing null mrp-1 mutation and labelling mrp-1 protein. Intestinal specific Pges-1 promoter drives MRP-1 expression. Translational fusion construct. pPD95.75_Pges-1::mrp-1(isoform C cDNA)::gfp.DepositorInsertMRP-1 (mrp-1 Nematode)
UseTagsGFPExpressionWormMutationPromoterPges-1 (3.3kb)Available sinceJune 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
NG1237 pCI-TPI-PTC160-4H
Plasmid#65804PurposeNMD reporter mRNADepositorInsertTPI (TPI1 Human)
UseTags4H (4 copies of short beta-globin 3'UTR for …ExpressionMammalianMutationpremature stop codon at 160PromoterCMVAvailable sinceSept. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pOCC94
Plasmid#118912Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with honey bee melitine secretion signal, N-terminal MBP, cleavable with 3C protease, and C-terminal eGFP-HIS6DepositorInsertNcoI-HBMss-MBP-3C-NotI-ccdB-AscI-eGFP-HIS6-stop-HindIII cassette
UseTagsMBP, cleavable with 3C protease and eGFP-HIS6ExpressionInsectMutationPromoterpolHAvailable sinceJan. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
VRER variant
Plasmid#138561PurposeExpresses human codon-optimized SpCas9-VRER and blasticidin resistance: EFS promoter-VRER-NLS-FLAG-P2A-BSDDepositorInsertVRER
UseCRISPR and LentiviralTagsBSD, FLAG, and NLSExpressionMammalianMutationD1135V, G1218R, R1335E, T1337RPromoterEFSAvailable sinceJune 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
Nanog-CreER targeting construct
Plasmid#59720PurposeCre-ER knockin targeting construct for mouse endogenous Nanog locusDepositorInsertcreERT2
UseMouse TargetingTagsExpressionMutationPromotermNanogAvailable sinceJan. 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pET28a-PKM2-H391Y
Plasmid#122692PurposeExpress His-tagged PKM2 (H391Y) protein in bacteria.DepositorInsertPyruvate kinase M2 isoform (PKM Human)
UseTags6His-tagExpressionBacterialMutationH391YPromoterAvailable sinceMarch 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4-hB90
Plasmid#18968DepositorInsertBrevican C-terminus (BCAN Human)
UseTagsExpressionMammalianMutationHuman brevican signal peptide aa 1-23 followed by…PromoterAvailable sinceDec. 12, 2008AvailabilityAcademic Institutions and Nonprofits only -
pIS1 DICER1 long-mut-let-7
Plasmid#21651DepositorInsertDicer 1 (DICER1 Human)
UseLuciferaseTagsLuciferaseExpressionMammalianMutationMutant let-7 microRNA binding sitesPromoterAvailable sinceJuly 22, 2009AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-ChRger1-TS-EYFP
Plasmid#127243PurposeHigh photocurrent, low-light sensitive channelrhodopsin (ChRger1) for optogenetic activation with systemic delivery or for low-light activation. Driven by human Synapsin I promoter.DepositorInsertChRger1-TS-EYFP
UseAAVTagsEYFP and SpyTagExpressionMammalianMutationPromoterhSyn1Available sinceSept. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pProvector_AAV2_586_PLGLAR
Plasmid#129024PurposeRecombinant AAV2 rep/cap plasmid modified to include an 18 amino acid peptide at cap position 586.DepositorInsertRep2/Cap2 with PLGLAR lock at 586
UseAAVTagsExpressionMutationinserted peptide motif (PLGLARGDDDDGPLGLAR) at po…PromoterAvailable sinceMay 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1a-delta-TMD-VAMP1-IRES-Hygromycin
Plasmid#134866PurposeExpresses EGFP-tagged mutant of VAMP1DepositorInsertVesicle-associated membrane protein 1 (VAMP1 Human)
UseLentiviralTagsEGFPExpressionMammalianMutationdeleted amino acids 97-118PromoterEF1aAvailable sinceJan. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA-CUL1 delta 610-615
Plasmid#19941DepositorInsertcullin 1 delta 610-615 (CUL1 Human)
UseTagsHAExpressionMammalianMutationCUL1 with a ROC1 binding mutantPromoterAvailable sinceApril 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
MDH1-miR-222-PGK-GFP-Blast
Plasmid#31522DepositorInsertmiR-222 (Mir222 Mouse)
UseRetroviralTagsGFPExpressionMammalianMutationPromoterAvailable sinceOct. 3, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCMV-hAR1-660
Plasmid#89079Purposemammalian expression of human androgen receptor N-terminal fragment 1-660 amino acidsDepositorInserthAR-1-660 (AR Human)
UseTagsExpressionMammalianMutationaa 1-660 (contains exons A,B,C and part of D)PromoterCMVAvailable sinceApril 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pIS1 DICER1 long-mut-all
Plasmid#21653DepositorInsertDicer 1 (DICER1 Human)
UseLuciferaseTagsLuciferaseExpressionMammalianMutationMutated let-7 and miR103/107 microRNA binding sit…PromoterAvailable sinceJuly 22, 2009AvailabilityAcademic Institutions and Nonprofits only -
CS2-Myc-hASUN
Plasmid#47955PurposeExpression of tagged-human ASUNDepositorInsertAsunder (INTS13 Human)
UseTagsMycExpressionMammalianMutationPromoterSp6Available sinceDec. 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
pIS1 DICER1 long-mut-miR103/107
Plasmid#21652DepositorInsertDicer 1 (DICER1 Human)
UseLuciferaseTagsLuciferaseExpressionMammalianMutationMutant miR103/107 microRNA binding sitesPromoterAvailable sinceJuly 22, 2009AvailabilityAcademic Institutions and Nonprofits only -
HOXC4 (human) MSCV
Plasmid#8546DepositorInsertHOXC4 (HOXC4 Human)
UseRetroviralTagsExpressionMammalianMutationContains improved ribosomal binding site and NcoI…PromoterAvailable sinceMay 30, 2006AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA-CUL1 L756A/I757A
Plasmid#19938DepositorInsertcullin 1 L756A/I757A (CUL1 Human)
UseTagsHA2ExpressionMammalianMutationMutation of two adjacent hydrophobic residues, L…PromoterAvailable sinceApril 17, 2009AvailabilityAcademic Institutions and Nonprofits only