Addgene

Showing: 5421 - 5440 of 18082 results

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pLCN DSB Repair Reporter (DRR)

Plasmid

#98895

Purpose

DSB Repair Reporter (DRR). Expresses GFP after cut of two inverted ISce1 sites and repair by NHEJ.

Depositor

Jan Karlseder

Article

Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

Insert

Neomycin-T2A-ISce1-ISce1-eGFP

Use

Lentiviral

Tags

Expression

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pCAGGS DRR mCherry Donor EF1a BFP

Plasmid

#98896

Purpose

Homologous recombination donor for pDRR. Expresses mCherry after repait by HR.

Depositor

Jan Karlseder

Article

Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

Insert

Neomycin-T2A-mCherry-GFP

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pIW601-KmCRISPR

Plasmid

#98907

Purpose

K. marxianus CRISPR Plasmid for sgRNA cloning

Depositor

Ian Wheeldon

Article

Lobs et al Biotechnol Biofuels. 2017 Jun 24;10:164.

Inserts

Codon optimized Cas9
sgRNA expression cassette

Use

CRISPR and Synthetic Biology

Tags

SV40

Expression

Yeast

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pXY027

Plasmid

#98915

Purpose

Express FtsZ tagged with GFP in E coli

Depositor

Jie Xiao

Article

Buss et al PLoS Genet. 2015 Apr 7;11(4):e1005128. d

Insert

FtsZ

Use

Tags

GFP

Expression

Bacterial

Mutation

Promoter

T5-lac

Availability

Academic Institutions and Nonprofits only

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pUbC-nls-ha-stdMCP-stdGFP

Plasmid

#98916

Purpose

synonymized tandem dimer MCP fused to synonymized tandem dimer GFP

Depositor

Robert Singer

Article

Wu et al Genes Dev. 2015 Apr 15;29(8):876-86. doi

Insert

stdMCP-stdGFP

Use

Lentiviral

Tags

GFP

Expression

Mutation

Promoter

UbC

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG.Ruby2sm-Flag.WPRE.SV40

Plasmid

#98925

Purpose

AAV expression of Ruby2 spaghetti monster FLAG construct

Depositor

Loren Looger

Article

Viswanathan et al Nat Methods. 2015 Jun;12(6):568-76. doi:

Insert

Ruby2-smFP-Flag

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CBA

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG.GFPsm-myc.WPRE.SV40

Plasmid

#98926

Purpose

AAV expression of GFP spaghetti monster myc construct

Depositor

Loren Looger

Article

Viswanathan et al Nat Methods. 2015 Jun;12(6):568-76. doi:

Insert

GFP-smFP-myc

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CBA

Availability

Academic Institutions and Nonprofits only

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pENN.AAV.CAG.Flex.GFPsm_myc.WPRE.SV40

Plasmid

#98927

Purpose

Cre dependent AAV expression of GFP spaghetti monster myc construct

Depositor

Loren Looger

Article

Viswanathan et al Nat Methods. 2015 Jun;12(6):568-76. doi:

Has Service

AAV1

Insert

GFP-smFP-myc

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG.Flex.Ruby2sm-Flag.WPRE.SV40

Plasmid

#98928

Purpose

Cre dependent AAV expression of Ruby2 spaghetti monster FLAG construct

Depositor

Loren Looger

Article

Viswanathan et al Nat Methods. 2015 Jun;12(6):568-76. doi:

Has Service

AAV1

Insert

Ruby2-smFP-Flag

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Availability

Academic Institutions and Nonprofits only

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pAAV.hSyn.iGluSnFr.WPRE.SV40

Plasmid

#98929

Purpose

AAV expression of glutamate sensor from human synapsin promoter. ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2013 Feb;10(2):162-70. doi:

Has Service

AAV1, AAV5, and AAV9

Insert

iGluSnFr

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

hSyn

Availability

Academic Institutions and Nonprofits only

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pcDNA3-CXCR4

Plasmid

#98942

Purpose

Mammalian expression plasmid for untagged human CXCR4

Depositor

Erik Procko

Article

Heredia et al J Immunol. 2018 Apr 20. pii: jimmunol.18

Insert

CXCR4 (CXCR4 Human)

Use

Tags

Expression

Mammalian

Mutation

Full-length, wildtype

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pcDNA3-CCR5

Plasmid

#98943

Purpose

Mammalian expression plasmid for untagged human CCR5

Depositor

Erik Procko

Article

Heredia et al J Immunol. 2018 Apr 20. pii: jimmunol.18

Insert

CCR5 (CCR5 Human)

Use

Tags

Expression

Mammalian

Mutation

Full-length, wildtype

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pcDNA3-CXCL12-sfGFP

Plasmid

#98961

Purpose

Mammalian expression plasmid for superfolder GFP-fused human chemokine CXCL12

Depositor

Erik Procko

Article

Heredia et al J Immunol. 2018 Apr 20. pii: jimmunol.18

Insert

CXCL12 (CXCL12 Human)

Use

Tags

Superfolder GFP

Expression

Mammalian

Mutation

Full-length, wildtype

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pCAGGS Donor mClover-LMNA

Plasmid

#98970

Purpose

Donor for endogenous N-ter tagging of hLMNA with mClover. Cas9 Homologous Recombination Reporter

Depositor

Jan Karlseder

Article

Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

Insert

LMNA5'UTR-mClover-LMNA5'exon1 (LMNA Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pUC CBA-SpCas9.EF1a-BFP.sgLMNA

Plasmid

#98971

Purpose

Cas9 Homologous Recombination Reporter. SpCas9 and a sgRNA targeting LMNA. TagBFP.

Depositor

Jan Karlseder

Article

Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

Insert

LMNA sgRNA and spCas9

Use

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pcDNA3-TrxRFP1

Plasmid

#98996

Purpose

mammalian expression of a biosensor to monitor thioredoxin redox dynamics

Depositor

Huiwang Ai

Article

Fan et al Nat Chem Biol. 2017 Jun 26. doi: 10.1038

Insert

human thioredoxin 1, GS-rich linker, fused with a redox-sensitive RFP

Use

Tags

His-tag

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pMito-TrxRFP1

Plasmid

#98997

Purpose

mammalian expression of a biosensor to monitor thioredoxin redox dynamics, mitochondrially locaclized

Depositor

Huiwang Ai

Article

Fan et al Nat Chem Biol. 2017 Jun 26. doi: 10.1038

Insert

TrxRFP1 and mitochondrial localization sequence

Use

Tags

His-tag and Mitochondria localization sequence

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pProEx-IDE-wt

Plasmid

#99014

Purpose

Express human Insulin Degrading Enzyme (IDE) in E. coli.

Depositor

Wei-Jen Tang

Article

Shen et al Nature. 2006 Oct 19;443(7113):870-4. Epu

Insert

insulin degrading enzyme (IDE Human)

Use

Tags

His tag

Expression

Bacterial

Mutation

Promoter

TRC

Availability

Academic Institutions and Nonprofits only

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pcDNA-FLAG-SF2

Plasmid

#99021

Purpose

Mammalian expression of SF2

Depositor

Honglin Chen

Article

Huang et al Nat Commun. 2017 Mar 21;8:14751. doi: 10

Insert

SF2 (SRSF1 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pCX-V5-H7N9-NS1

Plasmid

#99022

Purpose

Mammalian expression of NS1

Depositor

Honglin Chen

Article

Huang et al Nat Commun. 2017 Mar 21;8:14751. doi: 10

Insert

NS1

Use

Tags

V5

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge