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pL

Plasmid

#196286

Purpose

35S promoter-driven expression of the positive-sense TSWV L RNA segment encoding codon-optimized L (RdRp)

Depositor

Zhenghe Li

Article

Liu et al Mol Plant. 2023 Mar 6;16(3):616-631. doi

Insert

Full length TSWV L antigenome encoding codon-optimized L (RdRp)

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

duplicated cauliflower mosaic virus 35S promoter

Available since

April 12, 2023

Availability

Academic Institutions and Nonprofits only

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pRV.GFP FOXP3

Plasmid

#13250

Depositor

Anjana Rao

Article

Wu et al Cell. 2006 Jul 28. 126(2):375-87.

Insert

FOXP3 (FOXP3 Human)

Use

Retroviral

Tags

Myc

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 3, 2006

Availability

Academic Institutions and Nonprofits only

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AAV-CMV-eGFP

Plasmid

#193022

Purpose

AAV vector plasmid expressing enhanced green fluorescent protein (eGFP) under the cytomegalovirus (CMV) promoter

Depositor

Bart Nieuwenhuis

Article

Nieuwenhuis et al Gene Ther. 2023 Jan 13. doi: 10.1038/s41

Insert

eGFP

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 20, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-SaPE2

Plasmid

#174817

Purpose

Mammalian expression of SaCas9 prime editor 2

Depositor

David Liu

Article

Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

Insert

SaPE2

Use

Tags

SV40 bpNLS

Expression

Mammalian

Mutation

Detailed in manuscript

Promoter

CMV

Available since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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GFP-BioID-FAM21

Plasmid

#121046

Purpose

Expresses GFP tagged BioID and human FAM21C in mammalian cells

Depositor

Gia Voeltz

Article

Hoyer et al Cell. 2018 Sep 20;175(1):254-265.e14. do

Inserts

BioID
FAM21C (FAM21C Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

R118G (highly promiscuous form)

Promoter

CMV

Available since

Feb. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pLIX-REST

Plasmid

#91896

Purpose

Doxycycline inducible expression of mouse REST

Depositor

Julien Sage

Insert

REST (Rest Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

TRE promoter, Tet ON

Available since

June 26, 2017

Availability

Academic Institutions and Nonprofits only

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pGL3-RARE-luciferase

Plasmid

#13458

Depositor

T. Michael Underhill

Article

Hoffman et al J Cell Biol. 2006 Jul 3. 174(1):101-13.

Insert

Retinoic Acid Receptor Response Element

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

Nov. 17, 2006

Availability

Academic Institutions and Nonprofits only

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CCR2-Tango

Plasmid

#66239

Purpose

Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

Depositor

Bryan Roth

Article

Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

Insert

CCR2 (CCR2 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only
pBBR_Cas9_sp1

Plasmid

#154851

Purpose

Expresses SpCas9 and sgRNA that targets the upp gene in Rhodobacter sphaeroides genome

Depositor

John van der Oost

Article

Mougiakos et al Microb Cell Fact. 2019 Nov 25;18(1):204.

Inserts

Spcas9 gene
sgRNA scaffold

Use

CRISPR

Tags

6xHis

Expression

Mutation

Codon harmonized variant for expression in Rhodob…

Promoter

Available since

Aug. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pSpCTRE-CD4

Plasmid

#114010

Purpose

Dox-inducible Cas9 expression with a CD4 selection marker

Depositor

Charles Rudin

Article

Hulton et al Nat Cancer. 2020 Mar;1(3):359-369. doi:

Insert

SpCas9

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 10, 2020

Availability

Academic Institutions and Nonprofits only

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pMH-HA

Plasmid

#101767

Purpose

Gateway-compatible destination vector for mammalian expression of N-terminal HA-tag fusion protein

Depositor

Michael Huen

Article

An et al Proc Natl Acad Sci U S A. 2017 Apr 4;114

Type

Empty backbone

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pBA560

Plasmid

#186236

Purpose

pTet-eLbuCas13a crRNA entry vector

Depositor

Jennifer Doudna

Article

Adler et al Nat Microbiol. 2022 Dec;7(12):1967-1979.

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pHAGE-CMV-hIL7-IRES-ZsGreen-W

Plasmid

#26532

Depositor

David Baltimore

Insert

IL7 (IL7 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 18, 2010

Availability

Academic Institutions and Nonprofits only

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Nuclear PancACe

Plasmid

#215706

Purpose

Expresses acetyl-CoA biosensor in the nucleus

Depositor

Katharine Diehl

Article

Smith et al bioRxiv 2023.12.31.573774

Insert

PancACe

Use

Lentiviral

Tags

FLAG, HA, and NLS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 12, 2024

Availability

Academic Institutions and Nonprofits only

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RNA motif plasmid EDEN15

Plasmid

#107252

Purpose

EDEN15 RNA motif

Depositor

Paul Khavari

Article

Ramanathan et al Nat Methods. 2018 Feb 5. pii: nmeth.4601

Insert

EDEN15 motif

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 15, 2018

Availability

Academic Institutions and Nonprofits only

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pLNCX2 ER:ras

Plasmid

#67844

Purpose

4-hydroxytamoxifen inducible ER:ras fusion protein

Depositor

Masashi Narita

Article

Young et al Genes Dev. 2009 Apr 1;23(7):798-803. doi

Insert

ER1a H-RasG12V fusion

Use

Tags

Expression

Mammalian

Mutation

G525R renders the HBD largely insensitive to 17ß‐…

Promoter

CMV

Available since

Aug. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pInt_attP1_sacB_kanR

Plasmid

#205301

Purpose

Integrating plasmid for ORBIT in E. coli with sacB for counter selection. Used for markerless or scarless modifications.

Depositor

Scott Saunders

Article

Saunders et al bioRxiv 2023

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Aug. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-sRGECO

Plasmid

#137125

Purpose

Viral expression of sRGECO

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has Service

AAV8

Insert

sRGECO

Use

AAV

Tags

Expression

Mutation

E217D

Promoter

EF1a

Available since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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GFP- SEC61B

Plasmid

#121159

Purpose

Mammalian expression of GFP tagged SEC61B, used as a general ER marker

Depositor

Christine Mayr

Article

Ma et al Cell. 2018 Oct 30. pii: S0092-8674(18)31

Insert

SEC61B (SEC61B Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 7, 2019

Availability

Academic Institutions and Nonprofits only

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CAS9PBKS

Plasmid

#68371

Purpose

CbAkt promoter driven Cas9-2A-GFP (from px458)

Depositor

Eric-Paul Bennett

Article

Lonowski et al Nat Protoc. 2017 Mar;12(3):581-603. doi:

Insert

Cas9

Use

Tags

2A-EGFP

Expression

Mammalian

Mutation

Promoter

CbAkt

Available since

Feb. 23, 2017

Availability

Academic Institutions and Nonprofits only

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