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pRSFDuet-1_VNp-StefinA

Plasmid

#182403

Purpose

Bacterial expression of Vesicle Nucleating peptide-stefinA fusion

Depositor

Dan Mulvihill

Article

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Insert

VNp-stefin (CSTA Human)

Use

Tags

Vesicle Nucleating peptide (VNp)

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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JPF0419d

Plasmid

#124020

Purpose

Encodes pCAG driving expression of multicistronic Lyn-tagged iRFP713, cytoplasmic mAzamiGreen, mCerulean-tethered p38 KTR, and Histone 2B fused to mScarlet in a PiggyBac destination vector

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

PB_pCAG-Lyn-iRFP713-P2A-NES-mAzamiGreen-P2A-p38KTR-mCerulean-P2A-H2B::mScarlet

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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HOXA10-MSCV

Plasmid

#20980

Depositor

Corey Largman

Article

Thorsteinsdottir et al Mol Cell Biol. 1997 Jan . 17(1):495-505.

Insert

HOXA10 (HOXA10 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 14, 2009

Availability

Academic Institutions and Nonprofits only
pIS1 DICER1 long UTR

Plasmid

#21649

Depositor

David Bartel

Article

Mayr et al Cell. 2009 Aug 21. 138(4):673-84.

Insert

Dicer 1 long UTR (DICER1 Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

Promoter

Available since

July 22, 2009

Availability

Academic Institutions and Nonprofits only

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pLPC-mCh-ACTB-P2A-EGFP

Plasmid

#165158

Purpose

Expresses an ACTB gene fragment with P2A, flanked by mCherry and EGFP, in Mammalian cells

Depositor

Taekjip Ha

Article

Zou et al Mol Cell. 2021 Apr 1;81(7):1553-1565.e8.

Insert

mCherry-ACTB-P2A-EGFP (ACTB Human)

Use

Retroviral

Tags

EGFP and mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 12, 2021

Availability

Academic Institutions and Nonprofits only

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mADAM10 deltaMP in pQCXIP cFLAG

Plasmid

#19138

Depositor

Zena Werb

Article

Lemieux et al J Biol Chem. 2007 May 18. 282(20):14836-

Insert

ADAM10 (Adam10 Mouse)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

delta 56-457

Promoter

Available since

Sept. 8, 2008

Availability

Academic Institutions and Nonprofits only
PD-L1-miSFIT-1x

Plasmid

#124685

Purpose

Tuning expression of PD-1

Depositor

Tudor Fulga

Article

Michaels et al Nat Commun. 2019 Feb 18;10(1):818. doi:

Insert

PD-L1 (CD274 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

minimal CMV

Available since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

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pExpreS2-1-GFP

Plasmid

#175363

Purpose

ZeoR overexpression of cytosolic GFP in Drosophila ExpreS2ion cells

Depositor

ExpreS2ion Biotechnologies

Article

Coker et al Front Bioeng Biotechnol. 2022 May 5;10:8

Insert

sgGFP

Use

Tags

Expression

Insect

Mutation

F64L, S65C, and I167T

Promoter

Fused Actin-HSP70

Available since

March 30, 2022

Availability

Academic Institutions and Nonprofits only

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pY005 (pcDNA3.1-hLb3Cpf1)

Plasmid

#69977

Purpose

Expresses humanized Lb3Cpf1

Depositor

Feng Zhang

Article

Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

Insert

hLb3Cpf1

Use

CRISPR

Tags

NLS & 3xHA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 8, 2015

Availability

Academic Institutions and Nonprofits only
pAAV-EF1a-DIO-H2B.APEX-P2A-EGFP

Plasmid

#182825

Purpose

Cre-dependent expression of H2B-APEX fusion

Depositor

Yevgenia Kozorovitskiy

Article

Dumrongprechachan et al Nat Commun. 2021 Aug 11;12(1):4855. doi:

Insert

H2B.APEX-P2A-EGFP

Use

AAV

Tags

EGFP reporter (P2A)

Expression

Mutation

Promoter

EF1a

Available since

May 16, 2022

Availability

Academic Institutions and Nonprofits only

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VQR variant

Plasmid

#138560

Purpose

Expresses human codon-optimized SpCas9-VQR and blasticidin resistance: EFS promoter-VQR-NLS-FLAG-P2A-BSD

Depositor

Hyongbum Kim

Article

Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

Insert

VQR

Use

CRISPR and Lentiviral

Tags

BSD, FLAG, and NLS

Expression

Mammalian

Mutation

D1135V, R1335Q, T1337R

Promoter

EFS

Available since

June 9, 2020

Availability

Academic Institutions and Nonprofits only

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pX601-AAV-CMV-NLS-ISTfu1-NLS-3xHA-bGHpA;U6-BbsI-reRNA

Plasmid

#205319

Purpose

All-in-one AAV vector expressing ISTfu1 TnpB and its reRNA

Depositor

Haoyi Wang

Article

Xiang et al Nat Biotechnol. 2023 Jun 29. doi: 10.103

Insert

humanized ISTfu1 TnpB

Use

AAV

Tags

3 x HA

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pRevTRE MKL1-N100 S449A/T450A/S454A

Plasmid

#19849

Depositor

Ron Prywes

Article

Muehlich et al Mol Cell Biol. 2008 Aug 11. ():.

Insert

MKL1-N100 S449A/T450A/S454A (MRTFA Human)

Use

Retroviral

Tags

3xFLAG

Expression

Mutation

deleted amino acids 1-100 and changed Serine 449,…

Promoter

Available since

Feb. 27, 2009

Availability

Academic Institutions and Nonprofits only
pDisplay-GRAB_OTmut

Plasmid

#185385

Purpose

Expresses the genetically-encoded fluorescent oxytocin(OT) control sensor GRAB_OTmut in mammalian cells

Depositor

Yulong Li

Article

Qian et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

GPCR activation based oxytocin control sensor GRAB_OTmut

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 29, 2022

Availability

Academic Institutions and Nonprofits only

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pJP-HBB-nLuc

Plasmid

#175431

Purpose

Expresses nLuc with 5' leader of human beta globin (HBB) in mammalian cells.

Depositor

Britt Glaunsinger

Article

Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

Insert

nLUC plus 5' HBB leader

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pclbw-PAGFPomp25

Plasmid

#58427

Purpose

Mammalian expression of photoactivatable GFP targeted to the mitochondrial outer membrane

Depositor

David Chan

Article

Mishra et al Cell Metab. 2014 Apr 1;19(4):630-41. doi

Insert

paGFP-omp25 (Synj2bp Mouse)

Use

Retroviral

Tags

PAGFP

Expression

Mammalian

Mutation

contains aa's 109-145 of mouse omp25

Promoter

cmv-bactin

Available since

Dec. 16, 2015

Availability

Academic Institutions and Nonprofits only
AAV-miniSOG-VAMP2-citrine

Plasmid

#50969

Purpose

AAV2 transfer vector containing the InSynC (miniSOG-VAMP2-citrine) construct

Depositor

Roger Tsien

Article

Lin et al Neuron. 2013 Jul 24;79(2):241-53. doi: 1

Insert

miniSOG-VAMP2-citrine (Vamp2 Mouse, Arabdopsis thaliana)

Use

AAV

Tags

citrine and miniSOG

Expression

Mutation

Promoter

human synapsin promoter

Available since

Feb. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pCMV iGluf

Plasmid

#105833

Purpose

Fast indicator for extracellular glutamate imaging

Depositor

Katalin Torok

Article

Helassa et al Proc Natl Acad Sci U S A. 2018 May 7. pi

Insert

iGluSnFR E25D variant

Use

Tags

Myc and PDGFR

Expression

Mammalian

Mutation

Changed Glu 25 to Asp

Promoter

CMV

Available since

June 18, 2018

Availability

Academic Institutions and Nonprofits only

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PRE-Hsp70BbCas9_1.3

Plasmid

#190797

Purpose

aka PRE-Hsp70BbCas9 or 2gypsyPRE-Hsp70Bb-Cas9-T2A-eGFP; heat-shock inducible Cas9-T2A-eGFP expression regulated by upstream Polycomb response elements with two gypsy insulator elements. Final product

Depositor

Omar Akbari

Article

Warsinger-Pepe et al G3 (Bethesda). 2023 Apr 11;13(4):jkad024

Insert

2gypsyPRE-Hsp70Bb-Cas9-T2A-eGFP

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pHAGE2-TetOminiCMV-Oct4

Plasmid

#136611

Purpose

Dox-inducible lentiviral vector expressing mouse Oct4

Depositor

Hans Schöler

Article

Velychko et al Cell Stem Cell. 2019 Oct 30. pii: S1934-

Insert

Oct4 (Pou5f1 Mouse)

Use

Lentiviral

Tags

Expression

Bacterial

Mutation

Promoter

tetO-miniCMV (dox-inducible)

Available since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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