Addgene

We narrowed to 1,088 results for: piggyBac

Showing: 521 - 540 of 1088 results

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NAX_CAGGS_CAS9_m4_VPR_KanR neo

Plasmid

#167885

Purpose

PiggyBac compatible plasmid expressing dCas9-VPR

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

dCas9-VPR

Use

CRISPR

Tags

Expression

Mutation

D10A, D839A, H840A, N863A

Promoter

Availability

Academic Institutions and Nonprofits only

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NAX_CAGGS_CAS9_m4_VPR_mcherry

Plasmid

#167886

Purpose

PiggyBac compatible plasmid expressing dCas9-VPR

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

dCas9-VPR

Use

CRISPR

Tags

Expression

Mutation

D10A, D839A, H840A, N863A

Promoter

Availability

Academic Institutions and Nonprofits only
NAX_CAGGS_CAS9_m4_VPR_Puro

Plasmid

#167887

Purpose

PiggyBac compatible plasmid expressing dCas9-VPR

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

dCas9-VPR

Use

CRISPR

Tags

Expression

Mutation

D10A, D839A, H840A, N863A

Promoter

Availability

Academic Institutions and Nonprofits only

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NAX_CAGGS_CAS9_VPR_KanR neo

Plasmid

#167893

Purpose

PiggyBac compatible plasmid expressing Cas9-VPR

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

Cas9-VPR

Use

CRISPR

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pPIDCB2

Plasmid

#169900

Purpose

Empty dox inducible vector with piggyBac, insulators, and a DTS. The constitutive mClover3 is fused to H2B to allow for visualization of the nucleus.

Depositor

Richard Schneider

Article

Chu et al Biol Open. 2020 Oct 6;9(10). pii: bio.05

Type

Empty backbone

Use

Avian expression

Tags

Expression

Mammalian

Mutation

Promoter

Dox inducible

Availability

Academic Institutions and Nonprofits only

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LTR5HS S. pyogenes scaffold CARGO array

Plasmid

#191316

Purpose

Contains an array of guide RNAs targeting LTR5Hs elements

Depositor

Joanna Wysocka

Article

Fuentes et al Elife. 2018 Aug 2;7. pii: 35989. doi: 10

Insert

Array of guide RNAs

Use

CRISPR

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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LTR5HS S. aureus scaffold CARGO array

Plasmid

#191317

Purpose

Contains an array of guide RNAs targeting LTR5Hs elements

Depositor

Joanna Wysocka

Article

Fuentes et al Elife. 2018 Aug 2;7. pii: 35989. doi: 10

Insert

Array of guide RNAs

Use

CRISPR

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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PB-Blast-Dest-LifeAct-mRuby

Plasmid

#194907

Purpose

To generate LifeAct-mRuby expressing stable cell lines with piggybac transposase co-expression and blastocidin selections.

Depositor

Clare Buckley

Article

Liang et al EMBO J. 2022 Aug 22:e111021. doi: 10.152

Insert

LifeAct

Use

Mouse Targeting

Tags

mRuby

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pPB-EF1a-MegaGate-DD-Hygro

Plasmid

#175267

Purpose

MegaGate destination with C-terminal DD tag and hygromycin resistance

Depositor

George Church

Article

Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

Type

Empty backbone

Use

Tags

Destabilization Domain

Expression

Mammalian

Mutation

Promoter

EF1a

Availability

Academic Institutions and Nonprofits only

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pPB-EF1a-MegaGate-HA-Hygro

Plasmid

#175268

Purpose

MegaGate destination with C-terminal HA tag and hygromycin resistance

Depositor

George Church

Article

Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

Type

Empty backbone

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

EF1a

Availability

Academic Institutions and Nonprofits only

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pPB-EF1a-MegaGate-HA-Blast

Plasmid

#175270

Purpose

MegaGate destination with C-terminal HA tag and blasticidin resistance

Depositor

George Church

Article

Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

Type

Empty backbone

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

EF1a

Availability

Academic Institutions and Nonprofits only

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pPB-EF1a-MegaGate-DD-Blast

Plasmid

#175271

Purpose

MegaGate destination with C-terminal DD tag and blasticidin resistance

Depositor

George Church

Article

Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

Type

Empty backbone

Use

Tags

Destabilization Domain

Expression

Mammalian

Mutation

Promoter

EF1a

Availability

Academic Institutions and Nonprofits only

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PB-hEF1a-MG

Plasmid

#175504

Purpose

All-in-one piggyBac transposon destination vector for hEF1a constitutive expression of Mega Gate cloned elements

Depositor

George Church

Article

Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

hEF1a

Availability

Academic Institutions and Nonprofits only

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pTS26_QUAS_CsChrimson

Plasmid

#175548

Purpose

Expresses the optogenetic channel CsChrimson off of a QUAS promoter and contains Piggybac terminal repeats for transposon-mediated integration

Depositor

Leslie Vosshall

Article

Sorrells et al Elife. 2022 May 12;11:e76663. doi: 10.75

Insert

CsChrimson

Use

Tags

tdTomato

Expression

Insect

Mutation

Promoter

QUAS

Availability

Academic Institutions and Nonprofits only

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pAS1_4x7SKPylT_EF1_Ub-BsmBI

Plasmid

#162801

Purpose

STELLA destination plasmid containing restriction sites for golden gate cloning. Suitable for amber suppression (4xMmaPylT cassette); for transient expression

Depositor

Simon Elsaesser

Article

Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

Insert

Ub-BsmBI

Use

Piggybac

Tags

Expression

Mammalian

Mutation

Promoter

EF1alpha

Availability

Academic Institutions and Nonprofits only

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pAS1_4x7SKPylT_EF1_Ub-BsmBI-HA

Plasmid

#162802

Purpose

STELLA destination plasmid with HA terminal tag; Suitable for amber suppression (4xMmaPylT cassette); for transient expression

Depositor

Simon Elsaesser

Article

Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

Insert

Ub-BsmBI-HA

Use

Piggybac

Tags

HA

Expression

Mammalian

Mutation

Promoter

EF1alpha

Availability

Academic Institutions and Nonprofits only

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pAS1_4xh7SKPylT_EF1_BsmBI_(C1A)-Intein_DD

Plasmid

#162811

Purpose

Control STELLA destination plasmid for C-terminal labelled proteins. Contains restriction sites for golden gate cloning.

Depositor

Simon Elsaesser

Article

Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

Insert

BsmBI_(C1A)-Intein_DD

Use

Piggybac

Tags

Expression

Mammalian

Mutation

Intein(Cys1,Ala)

Promoter

EF1alpha

Availability

Academic Institutions and Nonprofits only

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pAS1_4xh7SKPylT_EF1_NoBody*_Intein_DD

Plasmid

#162813

Purpose

STELLA plasmid for amber suppression of C-terminally labelled NoBody

Depositor

Simon Elsaesser

Article

Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

Insert

NoBody(C-TAG)-Intein-DD

Use

Piggybac

Tags

Expression

Mammalian

Mutation

NoBody(Opal69,Amber)

Promoter

EF1alpha

Availability

Academic Institutions and Nonprofits only

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pAS1_4xh7SKPylT_EF1_SMIM38*_Intein_DD

Plasmid

#162814

Purpose

STELLA plasmid for amber suppression of C-terminally labelled SMIM38

Depositor

Simon Elsaesser

Article

Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

Insert

SMIM38(C-TAG)-Intein-DD

Use

Piggybac

Tags

Expression

Mammalian

Mutation

SMIM38(Ochre52,Amber)

Promoter

EF1alpha

Availability

Academic Institutions and Nonprofits only

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pSCL.140

Plasmid

#184995

Purpose

Express Cas9 in human cells

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Insert

SpCas9

Use

Tags

SV40NLS

Expression

Mammalian

Mutation

Promoter

TetOn-3g

Availability

Academic Institutions and Nonprofits only

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