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Addgene

pAS
(Plasmid #140008)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 140008 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    PB510B-1
  • Backbone manufacturer
    System Bioscience
  • Vector type
    Mammalian Expression
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    4xPylT (Mma) cassette
  • Species
    Methanosarcina mazei
  • Insert Size (bp)
    3255
  • Mutation
    U25C
  • Promoter h7SK

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SfiI (unknown if destroyed)
  • 3′ cloning site SalI (unknown if destroyed)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAS was a gift from Simon Elsaesser (Addgene plasmid # 140008 ; http://n2t.net/addgene:140008 ; RRID:Addgene_140008)
  • For your References section:

    Methanomethylophilus alvus Mx1201 provides basis for mutual orthogonal pyrrolysyl tRNA/aminoacyl-tRNA synthetase pairs in mammalian cells. Meineke B, Heimgartner J, Lafranchi L, Elsasser SJ. ACS Chem Biol. 2018 Sep 27. doi: 10.1021/acschembio.8b00571. 10.1021/acschembio.8b00571 PubMed 30260624