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Plasmid#182575PurposeMammalian cell expression of SARS-CoV-2 Spike protein Delta variant, furin side replaced by GSAS, 2 Proline at 986 and 987 plus T19R-G142D-del156/157-R158G-L452R-T478K-D614G-P681R-D950NDepositorInsertSpike S-GSAS-B.1.617.2.v1 (T19R-G142D-Del156/157-R158G-L452R-T478K-D614G-P681R-D950N) (S Severe acute respiratory syndrome coronavirus 2)
UseTags2X Strep-Tag II, 8X His tag, and HRV3C cleavage s…ExpressionMammalianMutationEctodomain (AAs 1-1208); 682-685 (furin site = RR…PromoterAvailable sinceApril 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pαH-S-GSAS/EG.5.1
Plasmid#218668PurposeMammalian cell expression of SARS-CoV-2 Spike protein of OMICRON.EG.5.1 variantDepositorInsertpαH-S-GSAS/EG.5.1 (S )
UseTagsHRV 3C cleavage site (before tags) C terminal on …ExpressionMammalianMutationEctodomain (AAs 1-1208); 682-685 (furin site = RR…PromoterCMVAvailable sinceMay 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pαH-S-GSAS/EG.5
Plasmid#212990PurposeMammalian cell expression of SARS-CoV-2 Spike protein of OMICRON.EG5 variantDepositorInsertpαH-S-GSAS/EG.5 (S )
UseTagsHRV 3C cleavage site (before tags) C terminal on …ExpressionMammalianMutationEctodomain (AAs 1-1208); 682-685 (furin site = RR…PromoterCMVAvailable sinceMarch 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDGE1108
Plasmid#202772PurposeCRISPR-KO in Arabidopsis thalianaDepositorInsertsFAST marker
pRPS5a:zCas9_ttriple
ccdB cassette (BsaI-excisable)
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceAug. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSW068
Plasmid#140036PurposeKO of PCC 11901 fadD gene/expresses codon-optimized 'tesA gene from E. coli from inducible cLac143 promoterDepositorInsertTruncated thioesterase gene
UseTagsExpressionBacterialMutationPromotercLac143Available sinceApril 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSZT025
Plasmid#140033PurposeKO of PCC 7002 fadD gene/expresses codon-optimized 'tesA gene from E. coli from inducible cLac143 promoterDepositorInsertTruncated thioesterase gene
UseTagsExpressionBacterialMutationPromotercLac143Available sinceApril 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPRv1.mir451_KO
Plasmid#164689PurposeFor CRISPR knockout of miR-451 by lentiviral delivery of Cas9 and miR-451 gRNADepositorInsertmiR-451 CRISPR KO gRNA (MIR451A )
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
Neg. Ctrl_sgRNA
Plasmid#111298PurposeCRISPR KO murine neg. ctrl.DepositorInsertNegCtrl sgRNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
MsCLYBL_sgRNA1
Plasmid#111294PurposeCRISPR KO murine CLYBLDepositorInsertsgRNA1 against murine CLYBL
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
MsCLYBL_sgRNA6
Plasmid#111295PurposeCRISPR KO murine CLYBLDepositorInsertsgRNA6 against murine CLYBL
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceNov. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pX459-canis_PTGS2-4
Plasmid#184730PurposeCOX2-KO in MDCKDepositorInsertA gRNA targeting the dog COX2 gene.
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pX459-homo_PTGS1-1
Plasmid#184731PurposeCOX1-KO in HeLaDepositorInsertA gRNA targeting the human COX1 gene.
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pX459-homo_PTGS1-2
Plasmid#184732PurposeCOX1-KO in HeLaDepositorInsertA gRNA targeting the human COX1 gene.
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pX459-homo_PTGS2-3
Plasmid#184733PurposeCOX2-KO in HeLaDepositorInsertA gRNA targeting the human COX2 gene.
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pX459-homo_PTGS2-4
Plasmid#184734PurposeCOX2-KO in HeLaDepositorInsertA gRNA targeting the human COX2 gene.
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pX459-homo_PLA2G4A-1
Plasmid#184735PurposecPLA2-KO in HeLaDepositorInsertA gRNA targeting the human cPLA2 gene.
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pX459-homo_PLA2G4A-2
Plasmid#184736PurposecPLA2-KO in HeLaDepositorInsertA gRNA targeting the human cPLA2 gene.
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
LentiCRISPRv2-bleo-COX1-4
Plasmid#184722PurposeCOX1-KO in MDCKDepositorInsertA gRNA targeting the dog COX1 gene.
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
LentiCRISPRv2-hyg2-COX2-4
Plasmid#184723PurposeCOX2-KO in MDCKDepositorInsertA gRNA targeting the dog COX2 gene.
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
LentiCRISPRv2-bleo-PTGER2
Plasmid#184724PurposeEP2-KO in MDCKDepositorInsertA gRNA targeting the dog EP2 gene.
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only