Addgene

Showing: 521 - 540 of 158776 results

Results per page:

Sort results by:

qTAG-C-3xHA-Blast

Plasmid

#207718

Purpose

Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a 3xHA-2A-Blast cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
qTAG-N-Blast-V5

Plasmid

#207686

Purpose

Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Blast-2A-V5 cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pH6HTC_TKT-Halo

Plasmid

#175331

Purpose

Production of HaloTag fusion protein

Depositor

Claudia Kutter

Article

Atanasoai et al bioRxiv 2021.11.08.467743

Insert

TKT (TKT Human)

Use

Cell free expression

Tags

HaloTag-6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 8, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pEntry_HCoV-OC43-nsp12

Plasmid

#168949

Purpose

Gateway-compatible Entry vector

Depositor

Pascal Falter-Braun

Article

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

HCoV-OC43-nsp12 (ORF1ab )

Use

Gateway-compatible entry vector

Tags

Expression

Mutation

Promoter

Available since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
P2RY6-Tango

Plasmid

#66476

Purpose

Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

Depositor

Bryan Roth

Article

Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

Insert

P2RY6 (P2RY6 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only
pTrc99a-NoSS-TolC

Plasmid

#155179

Purpose

Expresses signal sequence-less TolC as inclusion bodies

Depositor

Joanna Slusky

Article

Budiardjo et al Elife. 2022 Feb 24;11. pii: 73297. doi:

Insert

TolC (tolC )

Use

Tags

Expression

Bacterial

Mutation

deleted residues 1-20, residue 21 mutated to Met …

Promoter

trc

Available since

Aug. 16, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-3c-rhVTN-N

Plasmid

#30225

Depositor

James Thomson

Article

Chen et al Nat Methods. 2011 Apr 10. ():.

Insert

rhVitronectin with N terminus cleavage (VTN Human)

Use

Tags

Expression

Bacterial

Mutation

deleted amino acids: 20*-62

Promoter

Available since

May 25, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
AiP13779 - pAAV-AiE0779m_3xC2-minBG-iCre(R297T)-BGHpA (Alias: CN3779)

Plasmid

#199776

Purpose

enhancer AAV for Cre recombinase expression in striatal direct pathway D1-MSNs

Depositor

The Allen Institute for Brain Science, Jonathan Ting

Article

Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

Has Service

AAV PHP.eB

Insert

iCre(R297T)

Use

AAV

Tags

Expression

Mutation

R297T for reduced recombinase efficiency.

Promoter

Available since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
5HT6-YFP-PIPK

Plasmid

#96810

Purpose

cilia-membrane-targeted phosphatidylinositol 4-phosphate 5-kinase

Depositor

Takanari Inoue

Article

Phua et al Cell. 2017 Jan 12;168(1-2):264-279.e15.

Insert

5HT6-YFP-PIPK

Use

Tags

YFP

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 1, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
mKate1.31-Lifeact-7

Plasmid

#54668

Purpose

Localization: Actin, Excitation: 588, Emission: 635

Depositor

Michael Davidson

Article

Shcherbo et al Biochem J. 2009 Mar 15;418(3):567-74. do

Type

Empty backbone

Use

Tags

LifeAct-mKate1.31

Expression

Mammalian

Mutation

Promoter

Available since

July 25, 2014

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
qTAG-N-Zeo-3xFLAG

Plasmid

#207706

Purpose

Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Zeo-2A-3xFLAG cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
qTAG-N-Blast-3xHA

Plasmid

#207685

Purpose

Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Blast-2A-3xHA cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pCW-Flag-CtIP-T847E

Plasmid

#71110

Purpose

mammalian expession of Flag-CtlP T847E mutation

Depositor

Daniel Durocher

Article

Orthwein et al Nature. 2015 Dec 9. doi: 10.1038/nature1

Insert

CtlP

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

T847E

Promoter

TRE promoter, Tet ON

Available since

Dec. 9, 2015

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pJH2685

Plasmid

#191372

Purpose

Punc-4-TeTx::mCherry unc-54 3' UTR C.elegans A MNs/others neurou expression of TeTx RFP

Depositor

Mei Zhen

Article

Lu et al Curr Biol. 2022 Nov 7;32(21):4631-4644.e

Insert

Tetanus Toxin Light Chain

Use

Tags

wCherry

Expression

Worm

Mutation

Promoter

Punc-4

Available since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223-PLXNA3

Plasmid

#23518

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PLXNA3 (PLXNA3 Human)

Use

Gateway donor vector

Tags

Expression

Mutation

L1655M

Promoter

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR221_SLC39A6

Plasmid

#132153

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

Giulio Superti-Furga, RESOLUTE Consortium

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC39A6 (SLC39A6 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 22, 2019

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
lis-1::L4440

Plasmid

#11336

Depositor

Guy Caldwell

Insert

lis-1 (lis-1 Nematode)

Use

RNAi

Tags

Expression

Worm

Mutation

Promoter

Available since

May 25, 2006

Availability

Academic Institutions and Nonprofits only
pGL3 1 kb prom and exon1 fragment CD274

Plasmid

#107006

Purpose

Modified Luciferase expression vector

Depositor

Julian Downward

Article

Coelho et al Immunity. 2017 Dec 19;47(6):1083-1099.e6

Insert

CD274 (CD274 Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 23, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
MXS_CMV::ZeoR-bGHpA

Plasmid

#62441

Purpose

MXS_chaining vector with CMV::ZeoR-bGHpA

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

resistance cassette against Zeocin with CMV Promoter

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pCR-XL-rAkap9

Plasmid

#196867

Purpose

Cloned rat (r) A-Kinase Anchoring Protein 9 (Akap9) ORF. Used as a template for Akap9 containing constructs

Depositor

Frank Bradke

Article

Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

Insert

Akap9 (Akap9 Rat)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Lac promoter and T7 promoter

Available since

May 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge