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Plasmid#50706PurposeFinal destination vectorDepositorInsertTALEN-FokI nuclease backbone
UseTALENTagsFlagExpressionMutationPromoterCMVAvailable sinceMay 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pENTR4_EWSR1_C2G_R650G
Plasmid#26371DepositorInsertEWSR1 (EWSR1 Human)
UseEntry vectorTagsExpressionMutationR650GPromoterAvailable sinceSept. 22, 2010AvailabilityAcademic Institutions and Nonprofits only -
pJC25R1+4stop
Plasmid#17723DepositorInsertSup35R1+4 (SUP35 Budding Yeast)
UseTagsExpressionBacterialMutationE.coli expression plasmid for NM-region of Sup35 …PromoterAvailable sinceApril 4, 2008AvailabilityAcademic Institutions and Nonprofits only -
pLN428 (FuGW-G5p-mKate2-1Pe )
Plasmid#105184PurposeModule 3 - GAL4 synthetic promoter (with five binding sites) drives the expression of mKate2 transcripts containing miR1-Bs(Pe)DepositorInsertG5p-mKate2-1Pe
UseLentiviral and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pLN434 (FuGW-G14p-mKate2-1Pe )
Plasmid#105188PurposeModule 3 - GAL4 synthetic promoter (with fourteen binding sites) drives the expression of mKate2 transcripts containing miR1-Bs(Pe)DepositorInsertG14p-mKate2-1Pe
UseLentiviral and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pSpCas9(BB)-2A-Puro (PX459) V2.0
Plasmid#62988PurposeCas9 from S. pyogenes with 2A-Puro, and cloning backbone for sgRNA (V2.0)DepositorHas ServiceCloning Grade DNAInserthSpCas9-2A-Puro V2.0
UseCRISPRTags2A-Puro and 3XFLAGExpressionMammalianMutationPromoterCbhAvailable sinceMarch 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRSETB/mStayGold
Plasmid#212017PurposemStayGold has an n1 adaptor at its N-terminus and is useful for C-terminal tagging. This construct is used also for expression of mStayGold itself.DepositorInsertmStayGold
UseTagsExpressionBacterialMutationPromoterT7Available sinceJan. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGPA1
Plasmid#115476PurposeGeneration of mariner-based transposon mutant libraries in Enterococcus faeciumDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
eNme2-C TadCBEd
Plasmid#193841PurposeExpress TadCBEd (with eNme2-C variant) in mammalian cellsDepositorInserteNme2-C TadCBEd
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
psigma1-EGFP
Plasmid#53611PurposeExpresses sigma1 of adaptor protein of AP1DepositorInsertsigma 1 (Ap1s1 Mouse)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceJuly 23, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHR-UCOE-SFFV-dCas9-mCherry-ZIM3-KRAB
Plasmid#154473PurposeExpresses dCas9 fused to mCherry and the KRAB domain of ZIM3DepositorInsertZIM3 (ZIM3 Human)
UseLentiviralTagsHAExpressionMammalianMutationIncludes ZIM3 aa 1-100PromoterSFFVAvailable sinceOct. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYFAC-CH4
Plasmid#168980PurposeAMA1 based expression in filamentous fungi.pYFAC-pyroA carrying PalcA-T1-PalcS/M-T2-PaldA expression cassette ; unique restriction site: PacI for PalcA, NotI for PalcS, AsiSI for PalcM, AscI for PaldADepositorTypeEmpty backboneUseSynthetic Biology; Heterologous expression in asp…TagsExpressionMutationPromoterAvailable sinceDec. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCas3-Amp
Plasmid#207998PurposeExpresses all necessary components of Type I-C CRISPR-Cas systemDepositorUseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCas3-Km
Plasmid#207999PurposeExpresses all necessary components of Type I-C CRISPR-Cas systemDepositorUseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EGFP-IRES-puro
Plasmid#128652Purposemammalian expression of EGFP with puromycin selectionDepositorInsertEGFP
UseLentiviralTagsN/AExpressionMammalianMutationWild typePromoterCMVAvailable sinceJan. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
Lenti-iCas9-neo
Plasmid#85400PurposeLentiviral vector encoding a doxycycline inducible EGFP reporter downstream of FLAG-tagged spCas9, separated by a P2A self-cleavage sequenceDepositorInsertFlag-iCas9-P2A-GFP
UseLentiviralTagsFlag-iCas9-P2A-GFPExpressionMutationPromoterAvailable sinceJan. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
MSGV1-1D3-28Z.1-3 mut
Plasmid#107227PurposeAnti-mouse CD19 CAR with CD3 ITAMs mutatedDepositorInsert1D3-28Z. 1-3 mut
UseRetroviralTagsNoneExpressionMutationNonePromoterAvailable sinceMarch 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
eGFP-KIF5B
Plasmid#172203PurposeExpresses KIF5B tagged with eGFP in mammalian cellsDepositorInsertKIF5B (Kif5b Mouse)
UseTagseGFPExpressionMammalianMutationPromoterCMVAvailable sinceNov. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
eGFP-KIF5B-Rigor
Plasmid#172204PurposeExpresses KIF5B G234A rigor mutant tagged with eGFP in mammalian cellsDepositorInsertKIF5B (Kif5b Mouse)
UseTagseGFPExpressionMammalianMutationPromoterCMVAvailable sinceAug. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCXWB-EBNA1
Plasmid#37624PurposeNon-replicating episomal expression of EBNA1, which enhances transfection efficiency and expression from episomal plasmidsDepositorInsertEBNA-1 (EBNA-1 human herpesvirus 4)
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceDec. 3, 2012AvailabilityAcademic Institutions and Nonprofits only