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Plasmid#175008PurposeCytosol-targeted Akt-STOPS.DepositorInsertmCherry-Akt-STOPS-NES
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationPromoterCMVAvailable sinceJan. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCS2+ EB3-mKate2
Plasmid#105940Purposein vitro transcriptionDepositorInsertEB3 (MAPRE3 Human)
UseTagsmKate2ExpressionBacterialMutationPromoterAvailable sinceMay 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pX330 Human 3' HP1a gRNA
Plasmid#127907PurposeWT Cas9 Vector targeting the 3' end of the human HP1a geneDepositorInsertgRNA for Human 3' HP1a
UseCRISPRTagsExpressionMutationPromoterAvailable sinceSept. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLV V5-LAP2beta
Plasmid#175112PurposeLentiviral expression of V5-tagged mouse TmpoDepositorInsertTmpo (Tmpo Mouse)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterAvailable sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
shPBRM1#32-Tet-pLKO-puro
Plasmid#107399PurposeLentiviral expression of shRNA targeting PBRMDepositorInsertshRNA targeting PBRM1 (PBRM1 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJune 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
NHA-hnRNPK
Plasmid#120389PurposeExpression of HA tagged hnRNPK-lambdaN fusionDepositorInserthnRNPK (HNRNPK Human)
UseTagsHA and LambdaNExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
shPBRM1#131-Tet-pLKO-puro
Plasmid#107400PurposeLentiviral expression of shRNA targeting PBRMDepositorInsertshRNA targeting PBRM1 (PBRM1 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMay 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
TUBG1
Plasmid#87954Purposeexpresses human gamma-tubulin with a GFP-tag on the N-terminal of the proteinDepositorInsertgamma-tubulin 1 (TUBG1 Human)
UseTagsGFPExpressionMammalianMutationwhole protein, The C-terminal is a sh-gamma-tubul…PromoterAvailable sinceMay 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-1_VNp6-StefinA
Plasmid#182404PurposeBacterial expression of Vesicle Nucleating peptide6-stefinA fusionDepositorInsertVNp6-stefin (CSTA Human)
UseTagsVesicle Nucleating peptide 6 (VNp6)ExpressionBacterialMutationPromoterT7Available sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHAGE2-EF1a-MCS-hPOT1_IRES-blast
Plasmid#166411Purposeexpress hPOT1 in mammalian cellsDepositorInserthPOT1 (POT1 Human)
UseLentiviralTagsmycExpressionMammalianMutationPromoteref-1aAvailable sinceMay 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
EYFP-NLS-actin-K68/284R
Plasmid#60616PurposeEncodes YFP and NLS tagged beta-actin with sumoylation resistant mutationsDepositorInsertBeta-actin (ACTB Human)
UseTagsEYFP and NLSExpressionMammalianMutationK68R, 284RPromoterAvailable sinceNov. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLV Tmem161a-V5
Plasmid#175149PurposeLentiviral expression of mouse Tmem161a-V5DepositorInsertTmem161a (Tmem161a Mouse)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterAvailable sinceJan. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Lyso-mCherry-Rheb(C181S)
Plasmid#192438PurposeEncodes mCherry-tagged, farnesylation-deficient Rheb C181S mutant targeted to lysosome membrane.DepositorInsertLyso-mCherry-Rheb(C181S) (RHEB Human)
UseTagsFull-length LAMP1 and mCherryExpressionMammalianMutationRheb Cys 181 mutated to Ser; abolishes C-terminal…PromoterCMVAvailable sinceDec. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUB-nSypHer (NLS:sypHer:NLS)
Plasmid#84731PurposeExpresses sypHer in plant cells-biosensor for pH targeted to NucleiDepositorInsertnSypHer
UseTagsNLS (nuclear localisation signal and NLS (nuclear…ExpressionPlantMutationPromoterubiqutin- 10 gene promoter (pUBQ10) of ArabidopsisAvailable sinceJuly 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
HOXB8 (murine) HIS-tag pET
Plasmid#8540DepositorInsertHOXB8 (Hoxb8 Mouse)
UseTagsHis and T7ExpressionBacterialMutationstarts at amino acid #2 (Ser)PromoterAvailable sinceAug. 7, 2006AvailabilityAcademic Institutions and Nonprofits only -
pBlue-ACTB-IR-IRES-Puro-pEF1α-EGFP-IR-ACTB
Plasmid#169921PurposeDNA donor plasmid for site-specific integration of IRES-Puro-pEF1a-EGFP cassette at the ACTB locus using Cas9-transposase fusion proteins.DepositorInsertIRES-Puro-pEF1a-EGFP
UseCRISPRTagsExpressionMutationPromoterEF1aAvailable sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBXNPHM3-Nb_MsbA#1
Plasmid#186428PurposePlasmid for bacterial expression of MsbA binding Nanobody Nb_MsbA#1DepositorInsertNanobody Nb_MsbA#1
UseAffinity Reagent/ AntibodyTags3C cleavage site, His Tag, Maltose Binding Protei…ExpressionMutationPromoterpBADAvailable sinceOct. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
GFP-LacI-HIPK2
Plasmid#179269PurposeExpresses GFP-LacI-HIPK2 fusion proteinDepositorInserthomeodomain interacting protein kinase 2 (HIPK2 Human)
UseTagsGFP-LacIExpressionMammalianMutationPromoterCMVAvailable sinceMarch 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCK354
Plasmid#129698PurposeFor insulated expression in Synechocystis 6803. As pCK306 (rhaBAD rhamnose-inducible promoter, YFP, an E. coli-Synechocystis shuttle vector, chromosomal-integration sites) + terminator ECK120010799DepositorInsertsPrhaBAD
yfp
rhaS
Terminator ECK120010799
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSOMA-NLS
Plasmid#118937PurposeUsed to express the SOMA FRET sensor with a nuclear localization signal in Arabidopsis thalianaDepositorInsertSOMA Sensor for Arabidopsis MAPK activity with Nuclear Localization Signal
UseTagsExpressionPlantMutationPromoterAvailable sinceFeb. 20, 2019AvailabilityAcademic Institutions and Nonprofits only