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Plasmid#222729PurposeJ23119-BsaI_bRNA Golden Gate Landing Pad-HDV; T7-IS621 RecombinaseDepositorTypeEmpty backboneUseTags6xHisExpressionBacterialMutationPromoterAvailable sinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only
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pETcon_SARS-CoV-2_RBD
Plasmid#166782PurposeYeast surface display vector, expressing the SARS-2 RBD, 201aa constructDepositorInsertSpike receptor binding domain (RBD) from SARS-CoV-2 (S )
UseTagsExpressionYeastMutationPromoterAvailable sinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)_SpyCatcher-6aa-sfCherry2(1-10)
Plasmid#117484PurposeExpresses tandem binder (SpyCatcher002-6aa-sfCherry2(1-10)) in mammalian cellsDepositorInsertSpyCatcher002-6aa-sfCherry2(1-10)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV-AAV5cap
Plasmid#198015PurposeFor high-level expression of the AAV5 cap gene, suitable for use with pCMV-Rep78/68 SSM libraryDepositorInsertAAV5 cap
UseAAVTagsExpressionMammalianMutationWTPromoterCMVAvailable sinceMay 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAVS1-P-CAG-GFP
Plasmid#80491Purposedonor vector for AAVS1 targeting (puromycin selection) and constitutive GFP expressionDepositorInsertCAG-GFP
UseDonor vector (human)TagsExpressionMammalianMutationPromoterCAGAvailable sinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCMV-GR11
Plasmid#89105Purposemammalian expression of full-length human glucocorticoid receptor in pCMV5DepositorInserthuman glucocorticoid receptor (NR3C1 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
TFORF1845
Plasmid#143325PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertALX4 (ALX4 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
Gi2-CASE
Plasmid#168121PurposeEncodes a BRET-based activity sensor for the heterotrimeric G protein Gi2. Composed of the subunits G alpha i2 (GNAI2) tagged with NanoLuciferase, G beta 1 (GNB1) and Venus-tagged G gamma 2 (GNG2).DepositorUseLuciferaseTagscpVenus on GNG2ExpressionMammalianMutationNLuc is inserted at C112/E115 within GNAI2PromoterAvailable sinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
MBD2a pcDNA3.1
Plasmid#78141PurposeMammalian expression of MBD2aDepositorInsertMBD2a (MBD2 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
ERmoxGFP
Plasmid#68072Purposemammalian expression of ER localized moxGFPDepositorInsertmoxGFP
UseTagsKDEL ER retention sequence and bovine prolactin s…ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
MSCV-human-c-MYC-ER-mouse IRES-mcherry
Plasmid#73570PurposeMammalian retroviral expression of Myc-ERDepositorInsertcMYC (MYC Human)
UseRetroviralTagsIRES-mCherry and Mouse ERExpressionMammalianMutationPromoterMSCVAvailable sinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
TFORF3507
Plasmid#144983PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertNOCT (NOCT Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSL1119 (pDonor)
Plasmid#160732PurposeMini-transposon donor plasmid for V. cholerae CAST system. pUC19 vector backbone.DepositorInsertVchCAST donor DNA (Mini-Tn)
UseTagsExpressionBacterialMutationPromoterN/AAvailable sinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
Frt-V5-hspB1
Plasmid#63102PurposeExpression of HSPB1 (small HSP) tagged with V5 in mammalian cellsDepositorInserthspb1 (HSPB1 Human)
UseTagsV5ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pKL2351
Plasmid#199721PurposeA pVS1-based T-DNA binary vector and destination vector for Gateway cloning.DepositorInsertNPTII
UseTagsExpressionPlantMutationPromoterAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFX-Tom20-EGFP
Plasmid#174186PurposeExpress EGFP with mitochondria-targeting sequence in mammalian cellsDepositorInsertEGFP with mitchondria-targeting sequence
UseTagsthe mitochondria-targeting sequence of TOM20ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMA2640
Plasmid#25434DepositorInsertCytomegalovirus promoter-reverse tetracycline-regulated transactivator Advanced
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 21, 2010AvailabilityAcademic Institutions and Nonprofits only -
TFORF1346
Plasmid#143099PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertZNF541 (ZNF541 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pWUR703
Plasmid#53082Purposeinducible TtAgoDM (D478A,D546A) expressionDepositorInsertTtAgo
UseTagsStrep-II tagExpressionBacterialMutationD478A D546APromoterT7Available sinceMay 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
9X NFAT-apha-MHC-Luc
Plasmid#51941Purpose9 copies of NFAT sites in minimal alphaMHC-pGL-3DepositorInserts9x NFAT binding site
alpha-MHC
UseLuciferaseTagsluciferaseExpressionMutationPromoterAvailable sinceMay 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBR_attB(bxb)_lox
Plasmid#183762PurposeBxb1-specific donor plasmid for the cloning of payloads <20 kbDepositorTypeEmpty backboneUseCre/Lox and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceSept. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-OTp-GCaMP6s
Plasmid#192945PurposeTo drive GCaMP6s under the control of mouse oxytocin promoterDepositorInsertGCaMP6s
UseAAVTagsExpressionMutationPromoterAvailable sinceDec. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yoEGFP-Kan
Plasmid#44900PurposeYeast tagging vector to create gene fusion with yeast optimized EGFP with Kan selectionDepositorInsertyoEGFP
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 9, 2013AvailabilityAcademic Institutions and Nonprofits only -
RpHLuorin2
Plasmid#180227Purposefor bacterial expression of recombinant ratiometric pHluorin2DepositorInsertRatiometric pHLuorin2
UseTagsHis-tag followed by thrombin cleavage siteExpressionBacterialMutationPromoterT7Available sinceMarch 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pMH0001
Plasmid#85969PurposeUCOE-SFFV-dCas9-BFP-KRABDepositorInsertdCas9
UseLentiviralTagsBFP-KRABExpressionMammalianMutationD10A, H840APromoterSFFVAvailable sinceFeb. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
TFORF3231
Plasmid#144707PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertZNF423 (ZNF423 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLentiCas9-T2A-GFP
Plasmid#78548PurposeCas9 protein with GFPDepositorInsertT2A-GFP
UseLentiviralTagsExpressionMammalianMutationPromoterIn frame with Cas9Available sinceSept. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
TFORF1114
Plasmid#143929PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertRCOR3 (RCOR3 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
DS-SPcas
Plasmid#48645PurposeBacterial S. pyogenes Cas9 (SP) + tracrRNA expression, cloDF13/spectinomycinDepositorInsertsCas9
tracrRNA precursor
UseCRISPRTagsExpressionMutationPromoterproC and tracrRNA promoterAvailable sinceDec. 2, 2013AvailabilityAcademic Institutions and Nonprofits only