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Addgene

pMA2640
(Plasmid #25434)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 25434 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pMA2636rc
  • Backbone manufacturer
    unpublished
  • Backbone size w/o insert (bp) 7209
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Cytomegalovirus promoter-reverse tetracycline-regulated transactivator Advanced
  • Alt name
    CMV- rtTA Advanced
  • Alt name
    Tet-On Advanced
  • Species
    H. sapiens (human); E. coli
  • Insert Size (bp)
    1529

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer n/a
  • 3′ sequencing primer n/a
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Clontech
  • Articles Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMA2640 was a gift from Mikhail Alexeyev (Addgene plasmid # 25434 ; http://n2t.net/addgene:25434 ; RRID:Addgene_25434)
  • For your References section:

    A retro-lentiviral system for doxycycline-inducible gene expression and gene knockdown in cells with limited proliferative capacity. Alexeyev MF, Fayzulin R, Shokolenko IN, Pastukh V. Mol Biol Rep. 2010 Apr . 37(4):1987-91. 10.1007/s11033-009-9647-7 PubMed 19655272