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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 25434 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepMA2636rc
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Backbone manufacturerunpublished
- Backbone size w/o insert (bp) 7209
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Vector typeMammalian Expression, Retroviral
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Selectable markersBlasticidin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameCytomegalovirus promoter-reverse tetracycline-regulated transactivator Advanced
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Alt nameCMV- rtTA Advanced
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Alt nameTet-On Advanced
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SpeciesH. sapiens (human); E. coli
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Insert Size (bp)1529
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer n/a
- 3′ sequencing primer n/a (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byClontech
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMA2640 was a gift from Mikhail Alexeyev (Addgene plasmid # 25434 ; http://n2t.net/addgene:25434 ; RRID:Addgene_25434) -
For your References section:
A retro-lentiviral system for doxycycline-inducible gene expression and gene knockdown in cells with limited proliferative capacity. Alexeyev MF, Fayzulin R, Shokolenko IN, Pastukh V. Mol Biol Rep. 2010 Apr . 37(4):1987-91. 10.1007/s11033-009-9647-7 PubMed 19655272