-
Plasmid#86852Purposemammalian expression of ATP8 with G2 mitochondrial targeting sequence and V5 tagDepositorInsertATP8 (ATP8 Human)
UseTagsATP5G2 (G2) mitochondrial targeting signal and V5ExpressionMammalianMutationcodon optimizedPromoterCMVAvailable sinceMay 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-p97-E578Q
Plasmid#85672PurposeExpression of human p97 E578Q mutant with N-terminal GFP tagDepositorInsertp97 (Vcp Rat)
UseTagsGFPExpressionMammalianMutationE578QPromoterCMVAvailable sinceMarch 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-LGC-53
Plasmid#86399PurposeMammalian expression of codon-optimized dopamine receptor LGC-53DepositorInsertLGC-53 (lgc-53 Synthetic, Nematode)
UseTagsExpressionMammalianMutationCodon optimized for mammalian expression; Signal …PromoterCMVAvailable sinceJan. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CamKIIα-CRY2PHR-P2A-CIBN-mCherry-VAMP2
Plasmid#178574PurposeLentiviral vector for CamKIIα::Opto-vTrapDepositorInsertCRY2PHR-P2A-CIBN-mCherry-VAMP2
UseLentiviralTagsExpressionMutationPromoterCaMKIIaAvailable sinceApril 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-1_VNp-mNeongreen-DARPinOFF7
Plasmid#182415PurposeBacterial expression of Vesicle Nucleating peptide-mNeongreen-DARPinOFF7 fusionDepositorInsertVNp-mNeongreen-DARPinOFF7
UseTagsVesicle Nucleating peptide (VNp) & mNeongreenExpressionBacterialMutationPromoterT7Available sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
U6GRNA
Plasmid#68370PurposegRNA scaffold with hU6 promoterDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterhU6Available sinceFeb. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
mCherry Codon 59 sgRNA
Plasmid#109431PurposeMLM3636 backbone containing a gRNA that guides Cas9 to codon 59 of mCherry in the ACE reporterDepositorInsertmCherry codon 59 gRNA
UseCRISPRTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pclbw-eGFPomp25
Plasmid#58424PurposeMammalian expression of GFP targeted to the mitochondrial outer membraneDepositorInserteGFP-omp25 (Synj2bp Mouse)
UseRetroviralTagsEGFPExpressionMammalianMutationcontains aa's 109-145 of mouse omp25PromoterCMV/b-actinAvailable sinceDec. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-SynI-CreOn-FlpOff-EGFP
Plasmid#176274PurposeViral vector for expression of EGFP in cells expressing Cre AND NOT Flp driven by the human Synapsin promoter.DepositorInsertEGFP
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterhuman Synapsin IAvailable sinceDec. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSCL.177
Plasmid#184998PurposeExpress -Eco1 EMX1 editing ncRNA and gRNADepositorInsertEco1: EMX1 targeting and editing ncRNA-gRNA (a1/a2 length: 27 v1), expressed constitutively from a pol III (H1) promoter
UseTagsSV40NLSExpressionMammalianMutationEMX1 donor, a1/a2 length extended for 27 bpPromoterH1Available sinceNov. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV sgRNA-Notch1 #1 GFP
Plasmid#106950PurposeLentivirus encoding sgRNA targeting murine Notch1. Includes GFP marker.DepositorInsertgRNA targeting Notch1 (Notch1 Mouse)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceDec. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-DIO-VMAT2-WPRE-pA
Plasmid#39339DepositorInsertSlc18a2 (Slc18a2 Mouse)
UseAAV; RTagsExpressionMutationMyc tag inserted in the first lumenal loop betwee…PromoterAvailable sinceOct. 9, 2012AvailabilityAcademic Institutions and Nonprofits only -
pSCL.180
Plasmid#185001PurposeExpress -Eco1 AAVS1 editing ncRNA and gRNADepositorInsertEco1: AAVS1 targeting and editing ncRNA-gRNA (a1/a2 length: 27 v1), expressed constitutively from a pol III (H1) promoter
UseTagsSV40NLSExpressionMammalianMutationAAVS1 donor, a1/a2 length extended for 27 bpPromoterH1Available sinceNov. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-DIO(ChRger2-TS-YFP)
Plasmid#127239PurposeHigh photocurrent, low-light sensitive channelrhodopsin (ChRger2) for optogenetic activation with systemic delivery or for low-light activation. Driven by the CAG promoter and double floxed.DepositorInsertChRger2-TS-EYFP
UseAAVTagsEYFP and SpyTagExpressionMammalianMutationPromoterCAGAvailable sinceSept. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLV U6-sgRNA UbC-GFP
Plasmid#106948PurposesgRNA cloning cassette using BsmBI/Esp3I enzymes with GFP markerDepositorInsertsgRNA BsmBI/Esp3I cloning site
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceDec. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-CKAR2
Plasmid#123348PurposeImproved FRET-based biosensor for monitoring Protein Kinase C activity.DepositorInsertCKAR2
UseTags6xHIS, Cerulean3, T7 tag (gene 10 leader), Xpress…ExpressionMammalianMutationPromoterCMVAvailable sinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-BG505.SOSIP-8his
Plasmid#111847PurposeMammalian expression plasmid for soluble BG505 SOSIP.664DepositorInsertHIV-1 Env (BG505 SOSIP.664)
UseTags8his purification tag and CD5 leader sequenceExpressionMammalianMutationCodon-optimized synthetic gene; has SOSIP mutatio…PromoterCMVAvailable sinceMarch 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
GST-BHMT-I/G
Plasmid#104442PurposeAutophagy cargo assayDepositorInsertBHMT (BHMT Human)
UseTagsGFP-myc GFP is from Aequorea victoria and GSTExpressionMammalianMutationN43A in GST (in a potential KFERQ-like sequence).…PromoterCMVAvailable sinceDec. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-mPCSK8
Plasmid#122677PurposeExpresses mouse PCSK8 in mammalian cellsDepositorInsertMus musculus membrane-bound transcription factor peptidase, site 1 (Mbtps1), transcript variant 2 (Mbtps1 )
UseTagsExpressionMammalianMutationPromoterT7Available sinceMay 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA-CUL1 K720A
Plasmid#19939DepositorInsertcullin 1 K720A (CUL1 Human)
UseTagsHA2ExpressionMammalianMutationCUL1 with a NEDD8 modification mutantPromoterAvailable sinceApril 17, 2009AvailabilityAcademic Institutions and Nonprofits only