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Plasmid#153418PurposeRetroviral (MSCV) expression of all four WT CD3 subunits, co-expressed with LSSmOrangeDepositorInsertmurine CD3 delta-gamma-epsilon-zeta subunits (Cd3d Mouse)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceJuly 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSH-EF1a-OsTIR1.F74A-mCherry-IRES-puro
Plasmid#216247PurposeExpress OsTIR1.F74A mutant with mCherry tag for AID2DepositorInsertOsTIR1.F74A
UseCRISPR and TALEN; Human safe harbor locus (a avs1…TagsmCherryExpressionMammalianMutationPromoterEF1aAvailable sinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-45b-HOXD13-IDR-mCherry
Plasmid#145280PurposeBacterial Expression of 6xHis HOXD13 IDR mCherry Fusion ProteinDepositorInsertHOXD13-IDR (HOXD13 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/mCherry_pBirA-HA_FKBP8
Plasmid#154457Purposeexpresses mCherry_pBirA-HA_FKBP8/biochemical assayDepositorInsertmCherry_pBirA-HA_FKBP8 (FKBP8 Human)
UseTagsExpressionMammalianMutationPromoterCMV-FAvailable sinceAug. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pET19-MTdTc302
Plasmid#207458PurposeBacterial expression of Terminal deoxynucleotidyl transferase (TdT) cysteine variant. Only one surface-exposed cysteine, at residue 302DepositorInsertMTdTc302 (Dntt Mouse)
UseTags10xHis-MBPExpressionBacterialMutationCys188Ala, Cys216Ser, Cys378Ala, and Cys438SerPromoterAvailable sinceSept. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-TBK1-C-FLAG-HA
Plasmid#131792PurposeLentiviral vector expressing WT TBK1 containing STOP codonDepositorInsertTBK1 WT (TBK1 Human)
UseLentiviralTagsSTOP codonExpressionMammalianMutationWT full lengthPromoterCMVAvailable sinceOct. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
P-Ef1a-RPL22-3XFlag-P2A-EGFP-T2A-Puro
Plasmid#170318PurposeExpresses FLAG and EGFP tagged RPL22, puromycin resistanceDepositorInsertRPL22 (RPL22 Human)
UseLentiviralTagsExpressionMutationNonePromoterAvailable sinceMay 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
phROSA26-Tet-HA-JSAP1_WT
Plasmid#208772PurposeTetracycline inducible expression vector for HA-JSAP1_WT, used as a donor plasmid for HA-JSAP1_WT knock-in at the human ROSA26 locusDepositorInsertJSAP1 (MAPK8IP3 Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceNov. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-hPPP3CA-FLAG
Plasmid#179134PurposeExpression vector of human protein phosphatase 3, catalytic subunit, alpha isoform (PPP3CA) tagged with FLAG at C-terminus, CAG promoter, rabbit globin poly(A) signal.DepositorInsertprotein phosphatase 3, catalytic subunit, alpha isoform (PPP3CA Human)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceJan. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
Chr1q_Cassette-Integration_gRNA
Plasmid#195126PurposegRNA in a third generation Cas9 vector with GFP, targeting location downstream of Chr1q_centromere-targeting_gRNA for positive-negative selection cassette integrationDepositorInsertChr1q gRNA
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1_Cre
Plasmid#201198PurposeAAV vector for cre expression under EF1 promoterDepositorInsertscre
EF1alpha promoter
UseAAVTagsExpressionBacterial and MammalianMutationnuclear targeting crePromoterN/A and hEF1alpha promoterAvailable sinceJuly 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/tom20-pbira(79-c)-ha
Plasmid#154456Purposeexpresses tom20-pbira(79-c)-ha/biochemical assayDepositorInserttom20-pbira(79-c)-ha (TOMM20 Human)
UseTagsExpressionMammalianMutationPromoterCMV-FAvailable sinceAug. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
Dom neg CstF64
Plasmid#127250PurposeExpresses the Dominant Negative CstF64 in mammalian cells; DN blocks polyadenylationDepositorInsertCstF64 delta 282 (CSTF2 Human)
UseOtherTagsExpressionMammalianMutationinsert @SanD1:GTCCAGGCGCCTACCCATACGACGTCCCAGACTAC…PromoterpEF1aAvailable sinceJuly 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
PLX_307 FDX1
Plasmid#184495PurposeLentivirus expression for FDX1DepositorInsertFDX1 (FDX1 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHR-HOXD13-IDR-mCherry-CRY2
Plasmid#145258PurposeMammalian Expression of Opto HOXD13 IDRDepositorInsertHOXD13wt-IDR (HOXD13 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGem-nHyPer:cAPX
Plasmid#84736PurposeSimultaneous expresion of stromal HyPer2 and cytosolic Ascorbate peroxidase (APX) in plant cellsDepositorInsertsUseTagsNLS-SV40ExpressionPlantMutationPromoterpFMV/p35SAvailable sinceJune 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTEF:ATP
Plasmid#92179PurposeHO-TEF1p-ATeam1.03-KanMX4-HO. Codon optimized for yeast version of the ATeam1.03 ATP FRET sensor (originally from Imamura et al. PNAS, 2009 ), expressed via the TEF1 promoter. HO locus integration.DepositorInsertATeam1.03 codon optimized for yeast (Saccharomyces cerevisiae)
UseIntegration and expression into yeast.TagsYeast codon optimized mseCFP (donor) and cp173-mV…ExpressionYeastMutationPromoterTEF1pAvailable sinceJune 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCIG AKT3 E17K
Plasmid#73050PurposeHuman AKT3 E17KDepositorInsertAKT3 E17K (AKT3 Human)
UseTagsExpressionMammalianMutationE17KPromoterAvailable sinceApril 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAVTRE3_Clover
Plasmid#135179PurposeAAV expression of TRE(TRE3G)-driven, Clover expression for fluorescent lablingDepositorInsertsClover
TRE3G
UseAAVTagsExpressionMammalianMutationa variant of GFPPromoterTRE3GAvailable sinceJan. 10, 2020AvailabilityAcademic Institutions and Nonprofits only